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Extra tyrosine in the carbohydrate-binding module of Irpex lacteus Xyn10B enhances its cellulose-binding ability
http://hdl.handle.net/10091/00018612
http://hdl.handle.net/10091/00018612a03c305a-7eb2-4444-bb32-92e2dfccc0d2
名前 / ファイル | ライセンス | アクション |
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Extra_tyrosine_carbohydrate-binding_module.pdf (1.0 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2016-02-03 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Extra tyrosine in the carbohydrate-binding module of Irpex lacteus Xyn10B enhances its cellulose-binding ability | |||||
言語 | ||||||
言語 | eng | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | https://doi.org/10.1080/09168451.2014.996203 | |||||
関連名称 | 10.1080/09168451.2014.996203 | |||||
キーワード | ||||||
主題 | carbohydrate-binding module family 1, Irpex lacteus, glycoside hydrolase family 10 xylanase | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | journal article | |||||
著者 |
Nishijima, Hiroto
× Nishijima, Hiroto× Nozaki, Kouichi× Mizuno, Masahiro× Arai, Tsutomu× Amano, Yoshihiko |
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信州大学研究者総覧へのリンク | ||||||
氏名 | Nozaki, Kouichi | |||||
URL | http://soar-rd.shinshu-u.ac.jp/profile/ja.jpyFbpkh.html | |||||
信州大学研究者総覧へのリンク | ||||||
氏名 | Mizuno, Masahiro | |||||
URL | http://soar-rd.shinshu-u.ac.jp/profile/ja.gFDmbpkh.html | |||||
信州大学研究者総覧へのリンク | ||||||
氏名 | Amano, Yoshihiko | |||||
URL | http://soar-rd.shinshu-u.ac.jp/profile/ja.gVTVZVkh.html | |||||
出版者 | ||||||
出版者 | TAYLOR & FRANCIS LTD | |||||
出版者 | ||||||
出版者 | Japan Society for Bioscience, Biotechnology, and Agrochemistry | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY. 79(5):738-746 (2015) | |||||
書誌情報 |
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY 巻 79, 号 5, p. 738-746, 発行日 2015-05-04 |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | The xylanase (Xyn10B) that strongly adsorbs on microcrystalline cellulose was isolated from Driselase. The Xyn10B contains a Carbohydrate-binding module family 1 (CBM1) (IrpCBM(Xyn10B)) at N-terminus. The canonical essential aromatic residues required for cellulose binding were conserved in IrpCBM(Xyn10B); however, its adsorption ability was markedly higher than that typically observed for the CBM1 of an endoglucanase from Trametes hirsuta (ThCBMEG1). An analysis of the CBM-GFP fusion proteins revealed that the binding capacity to cellulose (7.8 mu mol/g) and distribution coefficient (2.0L/mu mol) of IrpCBM(Xyn10B)-GFP were twofold higher than those of ThCBMEG1-GFP (3.4 mu mol/g and 1.2L/mu mol, respectively), used as a reference structure. Besides the canonical aromatic residues (W24-Y50-Y51) of typical CBM1-containing proteins, IrpCBM(Xyn10B) had an additional aromatic residue (Y52). The mutation of Y52 to Ser (IrpCBM(Y52S)-GFP) reduced these adsorption parameters to 4.4 mu mol/g and 1.5L/mu mol, which were similar to those of ThCBMEG1-GFP. These results indicate that Y52 plays a crucial role in strong cellulose binding. | |||||
資源タイプ(コンテンツの種類) | ||||||
内容記述タイプ | Other | |||||
内容記述 | Article | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0916-8451 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA10824164 | |||||
PubMed | ||||||
識別子タイプ | PMID | |||||
関連識別子 | http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?CMD=search&DB=pubmed&term=25560084 | |||||
関連名称 | 25560084 | |||||
権利 | ||||||
権利情報 | This is an Accepted Manuscript of an article published by Taylor & Francis in 'Biochemistry & Molecular Biology' on 03 Jan 2015, available online: http://www.tandfonline.com/10.1080/09168451.2014.996203 . | |||||
出版タイプ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
WoS | ||||||
表示名 | Web of Science | |||||
URL | http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=ShinshuUniv&SrcApp=ShinshuUniv&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000354463700008 |