Item type |
学術雑誌論文 / Journal Article(1) |
公開日 |
2016-02-03 |
タイトル |
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タイトル |
Extra tyrosine in the carbohydrate-binding module of Irpex lacteus Xyn10B enhances its cellulose-binding ability |
言語 |
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言語 |
eng |
DOI |
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関連識別子 |
https://doi.org/10.1080/09168451.2014.996203 |
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関連名称 |
10.1080/09168451.2014.996203 |
キーワード |
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主題 |
carbohydrate-binding module family 1, Irpex lacteus, glycoside hydrolase family 10 xylanase |
資源タイプ |
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資源 |
http://purl.org/coar/resource_type/c_6501 |
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タイプ |
journal article |
著者 |
Nishijima, Hiroto
Nozaki, Kouichi
Mizuno, Masahiro
Arai, Tsutomu
Amano, Yoshihiko
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信州大学研究者総覧へのリンク |
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氏名 |
Nozaki, Kouichi |
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URL |
http://soar-rd.shinshu-u.ac.jp/profile/ja.jpyFbpkh.html |
信州大学研究者総覧へのリンク |
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氏名 |
Mizuno, Masahiro |
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URL |
http://soar-rd.shinshu-u.ac.jp/profile/ja.gFDmbpkh.html |
信州大学研究者総覧へのリンク |
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氏名 |
Amano, Yoshihiko |
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URL |
http://soar-rd.shinshu-u.ac.jp/profile/ja.gVTVZVkh.html |
出版者 |
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出版者 |
TAYLOR & FRANCIS LTD |
出版者 |
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出版者 |
Japan Society for Bioscience, Biotechnology, and Agrochemistry |
引用 |
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内容記述 |
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY. 79(5):738-746 (2015) |
書誌情報 |
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
巻 79,
号 5,
p. 738-746,
発行日 2015-05-04
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抄録 |
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内容記述 |
The xylanase (Xyn10B) that strongly adsorbs on microcrystalline cellulose was isolated from Driselase. The Xyn10B contains a Carbohydrate-binding module family 1 (CBM1) (IrpCBM(Xyn10B)) at N-terminus. The canonical essential aromatic residues required for cellulose binding were conserved in IrpCBM(Xyn10B); however, its adsorption ability was markedly higher than that typically observed for the CBM1 of an endoglucanase from Trametes hirsuta (ThCBMEG1). An analysis of the CBM-GFP fusion proteins revealed that the binding capacity to cellulose (7.8 mu mol/g) and distribution coefficient (2.0L/mu mol) of IrpCBM(Xyn10B)-GFP were twofold higher than those of ThCBMEG1-GFP (3.4 mu mol/g and 1.2L/mu mol, respectively), used as a reference structure. Besides the canonical aromatic residues (W24-Y50-Y51) of typical CBM1-containing proteins, IrpCBM(Xyn10B) had an additional aromatic residue (Y52). The mutation of Y52 to Ser (IrpCBM(Y52S)-GFP) reduced these adsorption parameters to 4.4 mu mol/g and 1.5L/mu mol, which were similar to those of ThCBMEG1-GFP. These results indicate that Y52 plays a crucial role in strong cellulose binding. |
資源タイプ(コンテンツの種類) |
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内容記述 |
Article |
ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
0916-8451 |
書誌レコードID |
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収録物識別子タイプ |
NCID |
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収録物識別子 |
AA10824164 |
PubMed |
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関連識別子 |
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?CMD=search&DB=pubmed&term=25560084 |
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関連名称 |
25560084 |
権利 |
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権利情報 |
This is an Accepted Manuscript of an article published by Taylor & Francis in 'Biochemistry & Molecular Biology' on 03 Jan 2015, available online: http://www.tandfonline.com/10.1080/09168451.2014.996203 . |
出版タイプ |
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出版タイプ |
AM |
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出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
WoS |
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URL |
http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=ShinshuUniv&SrcApp=ShinshuUniv&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000354463700008 |