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高速液体クロマトグラフィーによる血清および肝組織中α-トコフェロール(ビタミンE)定量法の検討
http://hdl.handle.net/10091/5172
http://hdl.handle.net/10091/517247305556-01e7-4be5-9e79-2a5c94ba4d93
名前 / ファイル | ライセンス | アクション |
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Allied_Med18_01.pdf (371.6 kB)
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Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2010-03-01 | |||||
タイトル | ||||||
言語 | ja | |||||
タイトル | 高速液体クロマトグラフィーによる血清および肝組織中α-トコフェロール(ビタミンE)定量法の検討 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Studies on the HPLC Method for Determination of α-Tocopherol (Vitamine E) in Serum and Liver Tissues | |||||
言語 | ||||||
言語 | jpn | |||||
キーワード | ||||||
主題 | Vitamin E, α-Tocopherol, HPLC, fluorospectrophotometry, allowable error limit | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | departmental bulletin paper | |||||
著者 |
寺澤, 文子
× 寺澤, 文子× 内川, 典子× 野本, 昭三 |
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出版者 | ||||||
出版者 | 信州大学医療技術短期大学部 | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 紀要 18: 1-8(1993) | |||||
書誌情報 |
紀要 巻 18, p. 1-8, 発行日 1993-02-28 |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | We established a HPLC method for determination of α-Tocopherol (Vitamin E) in serum and liver tissues. α-Tocopherol was extracted from serum and liver homogenate with a mixture of n-hexane and ethanol (4 : 1 v/v). The extracts were dried under gas flow of nitrogen at 40℃, and the residus were reconstituted with 200㎕ of 96% methanol. Twenty㎕ of the reconstituted specimen was injected into HPLC system equiped with reverse phase ODS column 150×4.6mm i.d., 20㎕ loop injector and fluorospectrophotometric detector (Ex.286nm, Em. 325nm) , and eluted with 96 % methanol. Within run precision, mean±SD and CV (obtained with this method) were 15.1± 0.36㎍/㎖, 2.4% for serum (n=15) and 23.9±1.20㎍/㎖, 5.0 % for liver tissues (n=10). The values of α-Tocopherol (mean±SD) in serum from healthy adults (n =20) and in liver tissues from mongrel dogs (n=-20) were 14.0±4.42㎍/㎖ and 20.7±7.41㎍/g , respectively. The method has an enough reproducibility and can be applied for determination of α-Tocopherol in biological materials in the field of laboratory medicine. | |||||
資源タイプ(コンテンツの種類) | ||||||
内容記述タイプ | Other | |||||
内容記述 | Article | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0385-1982 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AN10402438 |