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  1. 050 医学部, 大学院医学系研究科
  2. 0502 紀要・刊行物
  3. 05022 医療技術短期大学部紀要
  4. Vol. 18

高速液体クロマトグラフィーによる血清および肝組織中α-トコフェロール(ビタミンE)定量法の検討

http://hdl.handle.net/10091/5172
http://hdl.handle.net/10091/5172
47305556-01e7-4be5-9e79-2a5c94ba4d93
名前 / ファイル ライセンス アクション
Allied_Med18_01.pdf Allied_Med18_01.pdf (371.6 kB)
Item type 紀要論文 / Departmental Bulletin Paper(1)
公開日 2010-03-01
タイトル
タイトル 高速液体クロマトグラフィーによる血清および肝組織中α-トコフェロール(ビタミンE)定量法の検討
言語
言語 jpn
キーワード
主題Scheme Other
主題 Vitamin E
キーワード
主題Scheme Other
主題 α-Tocopherol
キーワード
主題Scheme Other
主題 HPLC
キーワード
主題Scheme Other
主題 fluorospectrophotometry
キーワード
主題Scheme Other
主題 allowable error limit
資源タイプ
資源 http://purl.org/coar/resource_type/c_6501
タイプ departmental bulletin paper
その他(別言語等)のタイトル
その他のタイトル Studies on the HPLC Method for Determination of α-Tocopherol (Vitamine E) in Serum and Liver Tissues
著者 寺澤, 文子

× 寺澤, 文子

WEKO 16840

寺澤, 文子

Search repository
内川, 典子

× 内川, 典子

WEKO 16841

内川, 典子

Search repository
野本, 昭三

× 野本, 昭三

WEKO 16842

野本, 昭三

Search repository
出版者
出版者 信州大学医療技術短期大学部
引用
内容記述タイプ Other
内容記述 紀要 18: 1-8(1993)
書誌情報 紀要

巻 18, p. 1-8, 発行日 1993-02-28
抄録
内容記述タイプ Abstract
内容記述 We established a HPLC method for determination of α-Tocopherol (Vitamin E) in serum and liver tissues. α-Tocopherol was extracted from serum and liver homogenate with a mixture of n-hexane and ethanol (4 : 1 v/v). The extracts were dried under gas flow of nitrogen at 40℃, and the residus were reconstituted with 200㎕ of 96% methanol. Twenty㎕ of the reconstituted specimen was injected into HPLC system equiped with reverse phase ODS column 150×4.6mm i.d., 20㎕ loop injector and fluorospectrophotometric detector (Ex.286nm, Em. 325nm) , and eluted with 96 % methanol. Within run precision, mean±SD and CV (obtained with this method) were 15.1± 0.36㎍/㎖, 2.4% for serum (n=15) and 23.9±1.20㎍/㎖, 5.0 % for liver tissues (n=10). The values of α-Tocopherol (mean±SD) in serum from healthy adults (n =20) and in liver tissues from mongrel dogs (n=-20) were 14.0±4.42㎍/㎖ and 20.7±7.41㎍/g , respectively. The method has an enough reproducibility and can be applied for determination of α-Tocopherol in biological materials in the field of laboratory medicine.
資源タイプ(コンテンツの種類)
内容記述タイプ Other
内容記述 Article
ISSN
収録物識別子タイプ ISSN
収録物識別子 0385-1982
書誌レコードID
収録物識別子タイプ NCID
収録物識別子 AN10402438
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