{"_buckets": {"deposit": "ff89e970-89a6-432f-81e2-521813f8b299"}, "_deposit": {"id": "6939", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "6939"}, "status": "published"}, "_oai": {"id": "oai:soar-ir.repo.nii.ac.jp:00006939"}, "item_10_alternative_title_1": {"attribute_name": "\u305d\u306e\u4ed6\uff08\u5225\u8a00\u8a9e\u7b49\uff09\u306e\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_alternative_title": "Studies on the HPLC Method for Determination of\u3000\u03b1-Tocopherol (Vitamine E) in Serum and Liver Tissues"}]}, "item_10_biblio_info_6": {"attribute_name": "\u66f8\u8a8c\u60c5\u5831", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "1993-02-28", "bibliographicIssueDateType": "Issued"}, "bibliographicPageEnd": "8", "bibliographicPageStart": "1", "bibliographicVolumeNumber": "18", "bibliographic_titles": [{"bibliographic_title": "\u7d00\u8981"}]}]}, "item_10_description_20": {"attribute_name": "\u6284\u9332", "attribute_value_mlt": [{"subitem_description": "We established a HPLC method for determination of \u03b1-Tocopherol (Vitamin E) in serum and liver tissues. \u03b1-Tocopherol was extracted from serum and liver homogenate with a mixture of n-hexane and ethanol (4 : 1 v/v). The extracts were dried under gas flow of nitrogen at 40\u2103, and the residus were reconstituted with 200\u3395 of 96% methanol. Twenty\u3395 of the reconstituted specimen was injected into HPLC system equiped with reverse phase ODS column 150\u00d74.6mm i.d., 20\u3395 loop injector and fluorospectrophotometric detector (Ex.286nm, Em. 325nm) , and eluted with 96 % methanol. Within run precision, mean\u00b1SD and CV (obtained with this method) were 15.1\u00b1 0.36\u338d/\u3396, 2.4% for serum (n=15) and 23.9\u00b11.20\u338d/\u3396, 5.0 % for liver tissues (n=10).  The values of \u03b1-Tocopherol (mean\u00b1SD) in serum from healthy adults (n =20) and in liver tissues from mongrel dogs (n=-20) were 14.0\u00b14.42\u338d/\u3396 and 20.7\u00b17.41\u338d/g , respectively. The method has an enough reproducibility and can be applied for determination of \u03b1-Tocopherol in biological materials in the field of laboratory medicine.", "subitem_description_type": "Abstract"}]}, "item_10_description_30": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7\uff08\u30b3\u30f3\u30c6\u30f3\u30c4\u306e\u7a2e\u985e\uff09", "attribute_value_mlt": [{"subitem_description": "Article", "subitem_description_type": "Other"}]}, "item_10_description_31": {"attribute_name": "\u30d5\u30a9\u30fc\u30de\u30c3\u30c8\uff1amime\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"subitem_description": "application/pdf", "subitem_description_type": "Other"}]}, "item_10_description_5": {"attribute_name": "\u5f15\u7528", "attribute_value_mlt": [{"subitem_description": "\u7d00\u8981 18: 1-8(1993)", "subitem_description_type": "Other"}]}, "item_10_publisher_4": {"attribute_name": "\u51fa\u7248\u8005", "attribute_value_mlt": [{"subitem_publisher": 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