Item type |
紀要論文 / Departmental Bulletin Paper(1) |
公開日 |
2010-03-01 |
タイトル |
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タイトル |
高速液体クロマトグラフィーによる血清および肝組織中α-トコフェロール(ビタミンE)定量法の検討 |
タイトル |
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タイトル |
Studies on the HPLC Method for Determination of α-Tocopherol (Vitamine E) in Serum and Liver Tissues |
言語 |
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言語 |
jpn |
キーワード |
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主題 |
Vitamin E, α-Tocopherol, HPLC, fluorospectrophotometry, allowable error limit |
資源タイプ |
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資源 |
http://purl.org/coar/resource_type/c_6501 |
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タイプ |
departmental bulletin paper |
著者 |
寺澤, 文子
内川, 典子
野本, 昭三
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出版者 |
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出版者 |
信州大学医療技術短期大学部 |
引用 |
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内容記述 |
紀要 18: 1-8(1993) |
書誌情報 |
紀要
巻 18,
p. 1-8,
発行日 1993-02-28
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抄録 |
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内容記述 |
We established a HPLC method for determination of α-Tocopherol (Vitamin E) in serum and liver tissues. α-Tocopherol was extracted from serum and liver homogenate with a mixture of n-hexane and ethanol (4 : 1 v/v). The extracts were dried under gas flow of nitrogen at 40℃, and the residus were reconstituted with 200㎕ of 96% methanol. Twenty㎕ of the reconstituted specimen was injected into HPLC system equiped with reverse phase ODS column 150×4.6mm i.d., 20㎕ loop injector and fluorospectrophotometric detector (Ex.286nm, Em. 325nm) , and eluted with 96 % methanol. Within run precision, mean±SD and CV (obtained with this method) were 15.1± 0.36㎍/㎖, 2.4% for serum (n=15) and 23.9±1.20㎍/㎖, 5.0 % for liver tissues (n=10). The values of α-Tocopherol (mean±SD) in serum from healthy adults (n =20) and in liver tissues from mongrel dogs (n=-20) were 14.0±4.42㎍/㎖ and 20.7±7.41㎍/g , respectively. The method has an enough reproducibility and can be applied for determination of α-Tocopherol in biological materials in the field of laboratory medicine. |
資源タイプ(コンテンツの種類) |
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内容記述 |
Article |
ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
0385-1982 |
書誌レコードID |
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収録物識別子タイプ |
NCID |
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収録物識別子 |
AN10402438 |