{"_buckets": {"deposit": "ff89e970-89a6-432f-81e2-521813f8b299"}, "_deposit": {"id": "6939", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "6939"}, "status": "published"}, "_oai": {"id": "oai:soar-ir.repo.nii.ac.jp:00006939", "sets": ["815"]}, "item_10_alternative_title_1": {"attribute_name": "その他（別言語等）のタイトル", "attribute_value_mlt": [{"subitem_alternative_title": "Studies on the HPLC Method for Determination of　α-Tocopherol (Vitamine E) in Serum and Liver Tissues"}]}, "item_10_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "1993-02-28", "bibliographicIssueDateType": "Issued"}, "bibliographicPageEnd": "8", "bibliographicPageStart": "1", "bibliographicVolumeNumber": "18", "bibliographic_titles": [{"bibliographic_title": "紀要"}]}]}, "item_10_description_20": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "We established a HPLC method for determination of α-Tocopherol (Vitamin E) in serum and liver tissues. α-Tocopherol was extracted from serum and liver homogenate with a mixture of n-hexane and ethanol (4 : 1 v/v). The extracts were dried under gas flow of nitrogen at 40℃, and the residus were reconstituted with 200㎕ of 96% methanol. Twenty㎕ of the reconstituted specimen was injected into HPLC system equiped with reverse phase ODS column 150×4.6mm i.d., 20㎕ loop injector and fluorospectrophotometric detector (Ex.286nm, Em. 325nm) , and eluted with 96 % methanol. Within run precision, mean±SD and CV (obtained with this method) were 15.1± 0.36㎍/㎖, 2.4% for serum (n=15) and 23.9±1.20㎍/㎖, 5.0 % for liver tissues (n=10).  The values of α-Tocopherol (mean±SD) in serum from healthy adults (n =20) and in liver tissues from mongrel dogs (n=-20) were 14.0±4.42㎍/㎖ and 20.7±7.41㎍/g , respectively. The method has an enough reproducibility and can be applied for determination of α-Tocopherol in biological materials in the field of laboratory medicine.", "subitem_description_type": "Abstract"}]}, "item_10_description_30": {"attribute_name": "資源タイプ（コンテンツの種類）", "attribute_value_mlt": [{"subitem_description": "Article", "subitem_description_type": "Other"}]}, "item_10_description_31": {"attribute_name": "フォーマット：mimeタイプ", "attribute_value_mlt": [{"subitem_description": "application/pdf", "subitem_description_type": "Other"}]}, "item_10_description_5": {"attribute_name": "引用", "attribute_value_mlt": [{"subitem_description": "紀要 18: 1-8(1993)", "subitem_description_type": "Other"}]}, "item_10_publisher_4": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "信州大学医療技術短期大学部"}]}, "item_10_select_64": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_select_item": "publisher"}]}, "item_10_source_id_35": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "0385-1982", "subitem_source_identifier_type": "ISSN"}]}, "item_10_source_id_39": {"attribute_name": "NII ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "0385-1982", "subitem_source_identifier_type": "ISSN"}]}, "item_10_source_id_40": {"attribute_name": "書誌レコードID", "attribute_value_mlt": [{"subitem_source_identifier": "AN10402438", "subitem_source_identifier_type": "NCID"}]}, "item_10_text_66": {"attribute_name": "sortkey", "attribute_value_mlt": [{"subitem_text_value": "01"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "寺澤,  文子"}], "nameIdentifiers": [{"nameIdentifier": "16840", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "内川,  典子"}], "nameIdentifiers": [{"nameIdentifier": "16841", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "野本,  昭三"}], "nameIdentifiers": [{"nameIdentifier": "16842", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2015-09-25"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "Allied_Med18_01.pdf", "filesize": [{"value": "371.6 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 371600.0, "url": {"label": "Allied_Med18_01.pdf", "url": "https://soar-ir.repo.nii.ac.jp/record/6939/files/Allied_Med18_01.pdf"}, "version_id": "51247393-a171-41a7-b0cd-e64f01f6df53"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "Vitamin E", "subitem_subject_scheme": "Other"}, {"subitem_subject": "α-Tocopherol", "subitem_subject_scheme": "Other"}, {"subitem_subject": "HPLC", "subitem_subject_scheme": "Other"}, {"subitem_subject": "fluorospectrophotometry", "subitem_subject_scheme": "Other"}, {"subitem_subject": "allowable error limit", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "jpn"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "高速液体クロマトグラフィーによる血清および肝組織中α-トコフェロール(ビタミンE)定量法の検討", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "高速液体クロマトグラフィーによる血清および肝組織中α-トコフェロール(ビタミンE)定量法の検討"}]}, "item_type_id": "10", "owner": "1", "path": ["815"], "permalink_uri": "http://hdl.handle.net/10091/5172", "pubdate": {"attribute_name": "公開日", "attribute_value": "2010-03-01"}, "publish_date": "2010-03-01", "publish_status": "0", "recid": "6939", "relation": {}, "relation_version_is_last": true, "title": ["高速液体クロマトグラフィーによる血清および肝組織中α-トコフェロール(ビタミンE)定量法の検討"], "weko_shared_id": null}