WEKO3
-
RootNode
アイテム
Significance of the Cgl1427 gene encoding cytidylate kinase in microaerobic growth of Corynebacterium glutamicum
http://hdl.handle.net/10091/17809
http://hdl.handle.net/10091/17809f4825db9-c291-487d-a354-82fca9c54479
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
Significance_Cgl1427_gene_encoding_cytidylate.pdf (765.7 kB)
|
|
| Item type | 学術雑誌論文 / Journal Article(1) | |||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 公開日 | 2014-08-06 | |||||||||||||||
| タイトル | ||||||||||||||||
| タイトル | Significance of the Cgl1427 gene encoding cytidylate kinase in microaerobic growth of Corynebacterium glutamicum | |||||||||||||||
| 言語 | ||||||||||||||||
| 言語 | eng | |||||||||||||||
| DOI | ||||||||||||||||
| 関連識別子 | https://doi.org/10.1007/s00253-012-4275-x | |||||||||||||||
| 関連名称 | 10.1007/s00253-012-4275-x | |||||||||||||||
| キーワード | ||||||||||||||||
| 主題 | Cytidylate kinase, Nucleotide synthesis, Oxygen, Microaerobic growth, Corynebacterium glutamicum | |||||||||||||||
| 資源タイプ | ||||||||||||||||
| 資源 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||||
| タイプ | journal article | |||||||||||||||
| 著者 |
Takeno, Seiki
× Takeno, Seiki
× Shirakura, Daisuke
× Tsukamoto, Nobuyoshi
× Mitsuhashi, Satoshi
× Ikeda, Masato
|
|||||||||||||||
| 信州大学研究者総覧へのリンク | ||||||||||||||||
| 氏名 | Takeno, Seiki | |||||||||||||||
| URL | http://soar-rd.shinshu-u.ac.jp/profile/ja.HULePUkh.html | |||||||||||||||
| 信州大学研究者総覧へのリンク | ||||||||||||||||
| 氏名 | Ikeda, Masato | |||||||||||||||
| URL | http://soar-rd.shinshu-u.ac.jp/profile/ja.uhLNPUkh.html | |||||||||||||||
| 出版者 | ||||||||||||||||
| 出版者 | SPRINGER | |||||||||||||||
| 引用 | ||||||||||||||||
| 内容記述 | APPLIED MICROBIOLOGY AND BIOTECHNOLOGY. 97(3):1259-1267 (2013) | |||||||||||||||
| 書誌情報 |
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 巻 97, 号 3, p. 1259-1267, 発行日 2013-02 |
|||||||||||||||
| 抄録 | ||||||||||||||||
| 内容記述 | The Cgl1427 gene was previously found to be relevant to the microaerobic growth of Corynebacterium glutamicum (Ikeda et al. Biosci Biotechnol Biochem 73:2806-2808, 2009). In the present work, Cgl1427 was identified as a cytidylate kinase gene (cmk) by homology analysis of its deduced amino acid sequence with that of other bacterial cytidylate kinases (CMP kinases) and on the basis of findings that deletion of Cgl1427 results in loss of CMP kinase activity. Deletion of the cmk gene significantly impaired the growth of C. glutamicum in oxygen-limiting static culture, and the impaired growth was restored by introducing a plasmid containing the cmk gene, suggesting that this gene plays an important role in the microaerobic growth of C. glutamicum. On the other hand, in the main culture with aerobic shaking, a prolonged lag phase was observed in the cmk disruptant, despite an unchanged growth rate, compared to the behavior of the wild-type strain. The prolongation was observed when using seed culture grown to later growth stages in which oxygen limitation occurred, but it was not observed when using seed culture grown to an earlier growth stage in which oxygen remained relatively plentiful. Since nucleotide biosynthesis in C. glutamicum requires oxygen, we hypothesized that the ability of the cmk disruptant to synthesize nucleotides was influenced by oxygen limitation in the later growth stages of the seed culture, which caused the prolongation of the lag phase in the following shaken culture. To verify this hypothesis, a plasmid containing genes encoding all components of a homologous ribonucleotide reductase, a key enzyme for nucleotide synthesis that requires oxygen for its reaction, was introduced into the cmk disruptant, which significantly ameliorated the lag phase prolongation. Furthermore, this experimental setup almost completely restored the growth of the cmk disruptant in the oxygen-limiting static culture. These results indicate that CMP kinase plays an important role in normal nucleotide biosynthesis under an oxygen-limiting environment. | |||||||||||||||
| 資源タイプ（コンテンツの種類） | ||||||||||||||||
| ISSN | ||||||||||||||||
| 収録物識別子タイプ | ISSN | |||||||||||||||
| 収録物識別子 | 0175-7598 | |||||||||||||||
| 書誌レコードID | ||||||||||||||||
| 収録物識別子タイプ | NCID | |||||||||||||||
| 収録物識別子 | AA10503386 | |||||||||||||||
| PubMed | ||||||||||||||||
| 識別子タイプ | PMID | |||||||||||||||
| 関連識別子 | https://pubmed.ncbi.nlm.nih.gov/22810301/ | |||||||||||||||
| 関連名称 | 22810301 | |||||||||||||||
| 権利 | ||||||||||||||||
| 権利情報 | The original publication is available at www.springerlink.com | |||||||||||||||
| 出版タイプ | ||||||||||||||||
| 出版タイプ | AM | |||||||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||||||||||||
| WoS | ||||||||||||||||
| URL | http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=ShinshuUniv&SrcApp=ShinshuUniv&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000314043300032 | |||||||||||||||
