ログイン
言語:

WEKO3

  • トップ
  • ランキング
To
lat lon distance
To

Field does not validate



インデックスリンク

インデックスツリー

メールアドレスを入力してください。

WEKO

One fine body…

WEKO

One fine body…

アイテム

{"_buckets": {"deposit": "7cfdad34-8354-44e7-94f0-fd26afcdf0cf"}, "_deposit": {"id": "10259", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "10259"}, "status": "published"}, "_oai": {"id": "oai:soar-ir.repo.nii.ac.jp:00010259", "sets": ["1017"]}, "author_link": ["32418", "32419", "32420", "32421", "32422", "32423", "32424", "32425", "32426"], "item_1628147817048": {"attribute_name": "出版タイプ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_ab4af688f83e57aa", "subitem_version_type": "AM"}]}, "item_6_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2013-08", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "15", "bibliographicPageEnd": "4594", "bibliographicPageStart": "4586", "bibliographicVolumeNumber": "79", "bibliographic_titles": [{"bibliographic_title": "APPLIED AND ENVIRONMENTAL MICROBIOLOGY"}]}]}, "item_6_description_20": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "To develop the infrastructure for biotin production through naturally biotin-auxotrophic Corynebacterium glutamicum, we attempted to engineer the organism into a biotin prototroph and a biotin hyperauxotroph. To confer biotin prototrophy on the organism, the cotranscribed bioBF genes of Escherichia coli were introduced into the C. glutamicum genome, which originally lacked the bioF gene. The resulting strain still required biotin for growth, but it could be replaced by exogenous pimelic acid, a source of the biotin precursor pimelate thioester linked to either coenzyme A (CoA) or acyl carrier protein (ACP). To bridge the gap between the pimelate thioester and its dedicated precursor acyl-CoA (or -ACP), the bioI gene of Bacillus subtilis, which encoded a P450 protein that cleaves a carbon-carbon bond of an acyl-ACP to generate pimeloyl-ACP, was further expressed in the engineered strain by using a plasmid system. This resulted in a biotin prototroph that is capable of the de novo synthesis of biotin. On the other hand, the bioY gene responsible for biotin uptake was disrupted in wild-type C. glutamicum. Whereas the wildtype strain required approximately 1 mu g of biotin per liter for normal growth, the bioY disruptant (Delta bioY) required approximately 1 mg of biotin per liter, almost 3 orders of magnitude higher than the wild-type level. The Delta bioY strain showed a similar high requirement for the precursor dethiobiotin, a substrate for bioB-encoded biotin synthase. To eliminate the dependency on dethiobiotin, the bioB gene was further disrupted in both the wild-type strain and the Delta bioY strain. By selectively using the resulting two strains (Delta bioB and Delta bioBY) as indicator strains, we developed a practical biotin bioassay system that can quantify biotin in the seven-digit range, from approximately 0.1 mu g to 1 g per liter. This bioassay proved that the engineered biotin prototroph of C. glutamicum produced biotin directly from glucose, albeit at a marginally detectable level (approximately 0.3 mu g per liter).", "subitem_description_type": "Abstract"}]}, "item_6_description_30": {"attribute_name": "資源タイプ(コンテンツの種類)", "attribute_value_mlt": [{"subitem_description": "Article", "subitem_description_type": "Other"}]}, "item_6_description_5": {"attribute_name": "引用", "attribute_value_mlt": [{"subitem_description": "APPLIED AND ENVIRONMENTAL MICROBIOLOGY. 79(15):4586-4594 (2013)", "subitem_description_type": "Other"}]}, "item_6_link_3": {"attribute_name": "信州大学研究者総覧へのリンク", "attribute_value_mlt": [{"subitem_link_text": "Ikeda, Masato", "subitem_link_url": "http://soar-rd.shinshu-u.ac.jp/profile/ja.uhLNPUkh.html"}, {"subitem_link_text": "Takeno, Seiki", "subitem_link_url": "http://soar-rd.shinshu-u.ac.jp/profile/ja.HULePUkh.html"}]}, "item_6_link_67": {"attribute_name": "WoS", "attribute_value_mlt": [{"subitem_link_text": "Web of Science", "subitem_link_url": "http://gateway.isiknowledge.com/gateway/Gateway.cgi?\u0026GWVersion=2\u0026SrcAuth=ShinshuUniv\u0026SrcApp=ShinshuUniv\u0026DestLinkType=FullRecord\u0026DestApp=WOS\u0026KeyUT=000321255600009"}]}, "item_6_publisher_4": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "AMER SOC MICROBIOLOGY"}]}, "item_6_relation_47": {"attribute_name": "PubMed", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "23709504"}], "subitem_relation_type_id": {"subitem_relation_type_id_text": "https://pubmed.ncbi.nlm.nih.gov/23709504/", "subitem_relation_type_select": "PMID"}}]}, "item_6_relation_48": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "10.1128/AEM.00828-13"}], "subitem_relation_type_id": {"subitem_relation_type_id_text": "https://doi.org/10.1128/AEM.00828-13", "subitem_relation_type_select": "DOI"}}]}, "item_6_rights_62": {"attribute_name": "権利", "attribute_value_mlt": [{"subitem_rights": "Copyright© 2013 American Society for Microbiology."}]}, "item_6_select_64": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_select_item": "author"}]}, "item_6_source_id_35": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "0099-2240", "subitem_source_identifier_type": "ISSN"}]}, "item_6_source_id_39": {"attribute_name": "NII ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "0099-2240", "subitem_source_identifier_type": "ISSN"}]}, "item_6_source_id_40": {"attribute_name": "書誌レコードID", "attribute_value_mlt": [{"subitem_source_identifier": "AA00543249", "subitem_source_identifier_type": "NCID"}]}, "item_6_text_70": {"attribute_name": "wosonly keywords", "attribute_value_mlt": [{"subitem_text_value": "BACILLUS-SUBTILIS; BREVIBACTERIUM-FLAVUM; CORYNEFORM BACTERIA; BIOSYNTHESIS PATHWAY; CYTOCHROME-P450 BIOI; GENES; CLONING; OPERON; GROWTH; EXPRESSION"}]}, "item_6_textarea_68": {"attribute_name": "wosonly abstract", "attribute_value_mlt": [{"subitem_textarea_value": "To develop the infrastructure for biotin production through naturally biotin-auxotrophic Corynebacterium glutamicum, we attempted to engineer the organism into a biotin prototroph and a biotin hyperauxotroph. To confer biotin prototrophy on the organism, the cotranscribed bioBF genes of Escherichia coli were introduced into the C. glutamicum genome, which originally lacked the bioF gene. The resulting strain still required biotin for growth, but it could be replaced by exogenous pimelic acid, a source of the biotin precursor pimelate thioester linked to either coenzyme A (CoA) or acyl carrier protein (ACP). To bridge the gap between the pimelate thioester and its dedicated precursor acyl-CoA (or -ACP), the bioI gene of Bacillus subtilis, which encoded a P450 protein that cleaves a carbon-carbon bond of an acyl-ACP to generate pimeloyl-ACP, was further expressed in the engineered strain by using a plasmid system. This resulted in a biotin prototroph that is capable of the de novo synthesis of biotin. On the other hand, the bioY gene responsible for biotin uptake was disrupted in wild-type C. glutamicum. Whereas the wildtype strain required approximately 1 mu g of biotin per liter for normal growth, the bioY disruptant (Delta bioY) required approximately 1 mg of biotin per liter, almost 3 orders of magnitude higher than the wild-type level. The Delta bioY strain showed a similar high requirement for the precursor dethiobiotin, a substrate for bioB-encoded biotin synthase. To eliminate the dependency on dethiobiotin, the bioB gene was further disrupted in both the wild-type strain and the Delta bioY strain. By selectively using the resulting two strains (Delta bioB and Delta bioBY) as indicator strains, we developed a practical biotin bioassay system that can quantify biotin in the seven-digit range, from approximately 0.1 mu g to 1 g per liter. This bioassay proved that the engineered biotin prototroph of C. glutamicum produced biotin directly from glucose, albeit at a marginally detectable level (approximately 0.3 mu g per liter)."}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Ikeda,  Masato"}], "nameIdentifiers": [{"nameIdentifier": "32418", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Miyamoto,  Aya"}], "nameIdentifiers": [{"nameIdentifier": "32419", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Mutoh,  Sumire"}], "nameIdentifiers": [{"nameIdentifier": "32420", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kitano,  Yuko"}], "nameIdentifiers": [{"nameIdentifier": "32421", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Tajima,  Mei"}], "nameIdentifiers": [{"nameIdentifier": "32422", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Shirakura,  Daisuke"}], "nameIdentifiers": [{"nameIdentifier": "32423", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Takasaki,  Manami"}], "nameIdentifiers": [{"nameIdentifier": "32424", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Mitsuhashi,  Satoshi"}], "nameIdentifiers": [{"nameIdentifier": "32425", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Takeno,  Seiki"}], "nameIdentifiers": [{"nameIdentifier": "32426", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2015-09-25"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "Development_Biotin-Prototrophic_Hyperauxotrophic_Corynebacterium.pdf", "filesize": [{"value": "924.5 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_note", "mimetype": "application/pdf", "size": 924500.0, "url": {"label": "Development_Biotin-Prototrophic_Hyperauxotrophic_Corynebacterium.pdf", "url": "https://soar-ir.repo.nii.ac.jp/record/10259/files/Development_Biotin-Prototrophic_Hyperauxotrophic_Corynebacterium.pdf"}, "version_id": "43959058-c519-404e-921e-c6a1de68b6ac"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Development of Biotin-Prototrophic and -Hyperauxotrophic Corynebacterium glutamicum Strains", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Development of Biotin-Prototrophic and -Hyperauxotrophic Corynebacterium glutamicum Strains", "subitem_title_language": "en"}]}, "item_type_id": "6", "owner": "1", "path": ["1017"], "permalink_uri": "http://hdl.handle.net/10091/17808", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2014-08-06"}, "publish_date": "2014-08-06", "publish_status": "0", "recid": "10259", "relation": {}, "relation_version_is_last": true, "title": ["Development of Biotin-Prototrophic and -Hyperauxotrophic Corynebacterium glutamicum Strains"], "weko_shared_id": -1}
  1. 070 農学部, 大学院農学研究科
  2. 0701 学術論文

Development of Biotin-Prototrophic and -Hyperauxotrophic Corynebacterium glutamicum Strains

http://hdl.handle.net/10091/17808
http://hdl.handle.net/10091/17808
d37c702f-558a-48c9-b038-b9f4e4bc6cf6
名前 / ファイル ライセンス アクション
Development_Biotin-Prototrophic_Hyperauxotrophic_Corynebacterium.pdf Development_Biotin-Prototrophic_Hyperauxotrophic_Corynebacterium.pdf (924.5 kB)
Item type 学術雑誌論文 / Journal Article(1)
公開日 2014-08-06
タイトル
言語 en
タイトル Development of Biotin-Prototrophic and -Hyperauxotrophic Corynebacterium glutamicum Strains
言語
言語 eng
資源タイプ
資源 http://purl.org/coar/resource_type/c_6501
タイプ journal article
著者 Ikeda, Masato

× Ikeda, Masato

WEKO 32418

Ikeda, Masato

Search repository
Miyamoto, Aya

× Miyamoto, Aya

WEKO 32419

Miyamoto, Aya

Search repository
Mutoh, Sumire

× Mutoh, Sumire

WEKO 32420

Mutoh, Sumire

Search repository
Kitano, Yuko

× Kitano, Yuko

WEKO 32421

Kitano, Yuko

Search repository
Tajima, Mei

× Tajima, Mei

WEKO 32422

Tajima, Mei

Search repository
Shirakura, Daisuke

× Shirakura, Daisuke

WEKO 32423

Shirakura, Daisuke

Search repository
Takasaki, Manami

× Takasaki, Manami

WEKO 32424

Takasaki, Manami

Search repository
Mitsuhashi, Satoshi

× Mitsuhashi, Satoshi

WEKO 32425

Mitsuhashi, Satoshi

Search repository
Takeno, Seiki

× Takeno, Seiki

WEKO 32426

Takeno, Seiki

Search repository
信州大学研究者総覧へのリンク
氏名 Ikeda, Masato
URL http://soar-rd.shinshu-u.ac.jp/profile/ja.uhLNPUkh.html
信州大学研究者総覧へのリンク
氏名 Takeno, Seiki
URL http://soar-rd.shinshu-u.ac.jp/profile/ja.HULePUkh.html
出版者
出版者 AMER SOC MICROBIOLOGY
引用
内容記述タイプ Other
内容記述 APPLIED AND ENVIRONMENTAL MICROBIOLOGY. 79(15):4586-4594 (2013)
書誌情報 APPLIED AND ENVIRONMENTAL MICROBIOLOGY

巻 79, 号 15, p. 4586-4594, 発行日 2013-08
抄録
内容記述タイプ Abstract
内容記述 To develop the infrastructure for biotin production through naturally biotin-auxotrophic Corynebacterium glutamicum, we attempted to engineer the organism into a biotin prototroph and a biotin hyperauxotroph. To confer biotin prototrophy on the organism, the cotranscribed bioBF genes of Escherichia coli were introduced into the C. glutamicum genome, which originally lacked the bioF gene. The resulting strain still required biotin for growth, but it could be replaced by exogenous pimelic acid, a source of the biotin precursor pimelate thioester linked to either coenzyme A (CoA) or acyl carrier protein (ACP). To bridge the gap between the pimelate thioester and its dedicated precursor acyl-CoA (or -ACP), the bioI gene of Bacillus subtilis, which encoded a P450 protein that cleaves a carbon-carbon bond of an acyl-ACP to generate pimeloyl-ACP, was further expressed in the engineered strain by using a plasmid system. This resulted in a biotin prototroph that is capable of the de novo synthesis of biotin. On the other hand, the bioY gene responsible for biotin uptake was disrupted in wild-type C. glutamicum. Whereas the wildtype strain required approximately 1 mu g of biotin per liter for normal growth, the bioY disruptant (Delta bioY) required approximately 1 mg of biotin per liter, almost 3 orders of magnitude higher than the wild-type level. The Delta bioY strain showed a similar high requirement for the precursor dethiobiotin, a substrate for bioB-encoded biotin synthase. To eliminate the dependency on dethiobiotin, the bioB gene was further disrupted in both the wild-type strain and the Delta bioY strain. By selectively using the resulting two strains (Delta bioB and Delta bioBY) as indicator strains, we developed a practical biotin bioassay system that can quantify biotin in the seven-digit range, from approximately 0.1 mu g to 1 g per liter. This bioassay proved that the engineered biotin prototroph of C. glutamicum produced biotin directly from glucose, albeit at a marginally detectable level (approximately 0.3 mu g per liter).
資源タイプ(コンテンツの種類)
内容記述タイプ Other
内容記述 Article
ISSN
収録物識別子タイプ ISSN
収録物識別子 0099-2240
書誌レコードID
収録物識別子タイプ NCID
収録物識別子 AA00543249
PubMed
識別子タイプ PMID
関連識別子 https://pubmed.ncbi.nlm.nih.gov/23709504/
関連名称 23709504
DOI
識別子タイプ DOI
関連識別子 https://doi.org/10.1128/AEM.00828-13
関連名称 10.1128/AEM.00828-13
権利
権利情報 Copyright© 2013 American Society for Microbiology.
出版タイプ
出版タイプ AM
出版タイプResource http://purl.org/coar/version/c_ab4af688f83e57aa
WoS
表示名 Web of Science
URL http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=ShinshuUniv&SrcApp=ShinshuUniv&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000321255600009
戻る
0
views
See details
Views

Versions

Ver.1 2021-03-01 12:16:47.803769
Show All versions

Share

Mendeley Twitter Facebook Print Addthis

Cite as

エクスポート

OAI-PMH
  • OAI-PMH JPCOAR
  • OAI-PMH DublinCore
  • OAI-PMH DDI
Other Formats
  • JSON
  • BIBTEX

Confirm


Powered by WEKO3


Powered by WEKO3