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Establishment and characterization of mouse bone marrow-derived mast cell hybridomas
http://hdl.handle.net/10091/17107
http://hdl.handle.net/10091/171073e8ad747-d10e-47ee-a837-3c169aeb1f4c
| 名前 / ファイル | ライセンス | アクション |
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Establishment_characterization_mouse_bone_marrow-derived_mast_cell_hybridomas.pdf (3.3 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||||
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| 公開日 | 2013-08-27 | |||||||
| タイトル | ||||||||
| タイトル | Establishment and characterization of mouse bone marrow-derived mast cell hybridomas | |||||||
| 言語 | ||||||||
| 言語 | eng | |||||||
| DOI | ||||||||
| 関連識別子 | https://doi.org/10.1016/j.yexcr.2012.07.010 | |||||||
| 関連名称 | 10.1016/j.yexcr.2012.07.010 | |||||||
| キーワード | ||||||||
| 主題 | Mast cell, Cell fusion, P815, Hybridoma, Degranulation, Toll-like receptor | |||||||
| 資源タイプ | ||||||||
| 資源 | http://purl.org/coar/resource_type/c_6501 | |||||||
| タイプ | journal article | |||||||
| 著者 |
Kawahara, Takeshi
× Kawahara, Takeshi
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| 信州大学研究者総覧へのリンク | ||||||||
| 氏名 | Kawahara, Takeshi | |||||||
| URL | http://soar-rd.shinshu-u.ac.jp/profile/ja.OVymPUkh.html | |||||||
| 出版者 | ||||||||
| 出版者 | ELSEVIER INC | |||||||
| 引用 | ||||||||
| 内容記述 | EXPERIMENTAL CELL RESEARCH. 318(18):2385-2396 (2012) | |||||||
| 書誌情報 |
EXPERIMENTAL CELL RESEARCH 巻 318, 号 18, p. 2385-2396, 発行日 2012-11 |
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| 抄録 | ||||||||
| 内容記述 | Interleukin (IL)-3-dependent mouse bone marrow-derived mast cells (BMMCs) are an important model for studying the function of mucosal-type mast cells. In the present study, BMMCs were successfully immortalized by cell fusion using a hypoxanthine-aminopterin-thymidine medium-sensitive variant of P815 mouse mastocytoma (P815-6TgR) as a partner cell line. The established mouse mast cell hybridomas (MMCHs) expressed alpha, beta, and gamma subunits of high-affinity immunoglobulin E (IgE) receptor (FcERI) and possessed cytoplasmic granules devoid of or partially filled with electron-dense material. Four independent MMCH clones continuously proliferated without supplemental exogenous IL-3 and showed a degranulation response on stimulation with IgE+antigen. Furthermore, histamine synthesis and release by degranulation were confirmed in MMCH-D5, a MMCH clone that showed the strongest degranulation response. MMCH-D5 exhibited elevated levels of 1L-3, IL-4, IL-13, granulocyte-macrophage colony-stimulating factor, tumor necrosis factor (TNF)-alpha, and cyclooxygenase 2, and production of prostaglandin D2 and leukotriene C4 in response to IgE-induced stimulation. MMCH clones also expressed Toll-like receptors (TLRs) 1, 2, 4, and 6 and showed elevated levels of TNF-alpha expression in response to stimulation with TLR2 and TLR4 ligands. The MMCHs established using this method should be suitable for studies on FcERI- and TLR-mediated effector functions of mast cells. | |||||||
| 資源タイプ（コンテンツの種類） | ||||||||
| ISSN | ||||||||
| 収録物識別子タイプ | ISSN | |||||||
| 収録物識別子 | 0014-4827 | |||||||
| 書誌レコードID | ||||||||
| 収録物識別子タイプ | NCID | |||||||
| 収録物識別子 | AA00641011 | |||||||
| PubMed | ||||||||
| 識別子タイプ | PMID | |||||||
| 関連識別子 | https://pubmed.ncbi.nlm.nih.gov/22824512/ | |||||||
| 関連名称 | 22824512 | |||||||
| 権利 | ||||||||
| 権利情報 | Copyright© 2012 Elsevier Inc. All rights reserved. | |||||||
| 出版タイプ | ||||||||
| 出版タイプ | AM | |||||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||||
| WoS | ||||||||
| URL | http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=ShinshuUniv&SrcApp=ShinshuUniv&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000308259900011 | |||||||
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Cite as
Kawahara, Takeshi, 2012, Establishment and characterization of mouse bone marrow-derived mast cell hybridomas: ELSEVIER INC, 2385–2396 p.
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