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Expression of the immunoreactive buckwheat major allergenic storage protein in Lactococcus lactis
http://hdl.handle.net/10091/16955
http://hdl.handle.net/10091/169558e8881bd-1f63-420c-8abf-6355668c5f61
| 名前 / ファイル | ライセンス | アクション |
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Expression_1_immunoreactive_buckwheat_major.pdf (1.2 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2013-04-23 | |||||
| タイトル | ||||||
| タイトル | Expression of the immunoreactive buckwheat major allergenic storage protein in Lactococcus lactis | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| DOI | ||||||
| 関連名称 | 10.1007/s00253-012-4608-9 | |||||
| キーワード | ||||||
| 主題 | Buckwheat, Fagopyrum esculentum, Allergen, Fagag1, Lactococcus lactis, Th2 | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| 著者 |
Shigemori, Suguru
× Shigemori, Suguru× Yonekura, Shinichi× Sato, Takashi× Otani, Hajime× Shimosato, Takeshi |
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| 信州大学研究者総覧へのリンク | ||||||
| 表示名 | Yonekura, Shinichi | |||||
| URL | http://soar-rd.shinshu-u.ac.jp/profile/ja.gakFPUkh.html | |||||
| 信州大学研究者総覧へのリンク | ||||||
| 表示名 | Shimosato, Takeshi | |||||
| URL | http://soar-rd.shinshu-u.ac.jp/profile/ja.ONLCjFkV.html | |||||
| 出版者 | ||||||
| 出版者 | SPRINGER | |||||
| 引用 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | APPLIED MICROBIOLOGY AND BIOTECHNOLOGY. 97(8):3603-3611 (2013) | |||||
| 書誌情報 |
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 巻 97, 号 8, p. 3603-3611, 発行日 2013-04 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Proteins from buckwheat (Fagopyrum esculentum) are strong allergens that can cause serious symptoms, including anaphylaxis, in patients with hypersensitivity. In this study, we successfully developed a modified lactic acid bacterial vector (pNSH) and a recombinant strain of Lactococcus lactis NZ9000 (NZ9000) that produced a major allergenic storage protein of buckwheat, Fagag1 (61.2 kDa, GenBank accession number AF152003), with or without a green fluorescent protein (GFP) tag. GFP fluorescence allows for rapid, simple, and accurate measurement of target protein expression by microscopy or fluorimetry. We describe a convenient method for production of rGFP-Fagag1 fusion and rFagag1 proteins with a good yield in an advantageous probiotic host. We found that in vitro treatment of splenocytes isolated from buckwheat crude protein-immunized mice with rFagag1 increased the expression of allergic inflammation cytokines such as IL-4, IL-13, and IL-17 F. Because it was less antigenic, rGFP-Fagag1 protein from NZ9000 might be of limited use; however, rFagag1 from NZ9000 evoked a robust response as measured by induction of IL-4 and IL-17 F expression levels. The observed allergic activity is indicative of a Th2 cell-mediated immune response and is similar to the effects induced by exposure to buckwheat crude protein. Our results suggest that expression of rFagag1 in NZ9000 may facilitate in vivo applications of this system aimed at improving the specificity of immunological responses to buckwheat allergens. | |||||
| 資源タイプ（コンテンツの種類） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Article | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0175-7598 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA10503386 | |||||
| PubMed | ||||||
| 識別子タイプ | PMID | |||||
| 関連識別子 | https://pubmed.ncbi.nlm.nih.gov/23212674/ | |||||
| 関連名称 | 23212674 | |||||
| 権利 | ||||||
| 権利情報 | The original publication is available at www.springerlink.com | |||||
| 出版タイプ | ||||||
| 出版タイプ | AM | |||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
| WoS | ||||||
| 表示名 | Web of Science | |||||
| URL | http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=ShinshuUniv&SrcApp=ShinshuUniv&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000317136700033 | |||||
