| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2008-01-15 |
| タイトル |
|
|
タイトル |
Quantitative Analysis of Rutin in Buckwheat (Fagopyrum sp.) by High Performance Liquid Chromatography |
| 言語 |
|
|
言語 |
eng |
| キーワード |
|
|
主題 |
Fagopyrum esculentum, Fagopyrum tataricum, buckwheat, rutin content, HPLC analysis |
| 資源タイプ |
|
|
資源 |
http://purl.org/coar/resource_type/c_6501 |
|
タイプ |
journal article |
| その他（別言語等）のタイトル |
|
|
その他のタイトル |
高速液体クロマトグラフィーによるソバ（Fagopyrum sp.）中のルチンの定量分析 |
|
言語 |
ja |
| 著者 |
Minami, M
Kitabayashi, H
Ujihara, A
|
| 出版者 |
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|
出版者 |
信州大学農学部 |
| 引用 |
|
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内容記述 |
信州大学農学部紀要. 34(2): 91-95 (1998) |
| 書誌情報 |
信州大学農学部紀要
巻 34,
号 2,
p. 91-95,
発行日 1998-03-23
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| 抄録 |
|
|
内容記述 |
For the quantitative analysis of rutin in common buckwheat (Fagopyrum esculentum) and tartary buckwheat (F. tataricum), the operating condition of high performance liquid chromatography (HPLC) and methods of sample preparation and extraction were investigated. Reliable analysis method with less than 5 g of sample was established as follows; ① drying samples for 24 hours at 70℃ by using forced air flow oven,② grinding 5 g of seed and 2 g of leaf samples into powder for 30 seconds,③ extracting rutin from 1.0 g of common buckwheat powder with 10 ml of methyl alcohol and from 0.5 mg of tartary buckwheat seed powder and common buckwheat leaf powder with 20 ml of methyl alcohol at 7O℃ for 30 min. by using Soxhelt extraction apparatus and water bath, and ④ injecting 20 μl of supernatant fluid obtained by centrifugation into chromatograph. The method has high reliability with about 5% of coefficient of variation in rutin content. Establishment of quantitative analysis method of rutin with small amount of seed sample less than 5 g by using HPLC will contribute to the progress of breeding for high rutin content buckwheat varieties. |
| 資源タイプ（コンテンツの種類） |
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| ISSN |
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収録物識別子タイプ |
ISSN |
|
収録物識別子 |
0583-0621 |
| 書誌レコードID |
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|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AN00121352 |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
v34n02_05.pdf (261.6 kB)
