{"_buckets": {"deposit": "bf4390a3-5ca3-419a-95d6-81035dcbc6d6"}, "_deposit": {"id": "13302", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "13302"}, "status": "published"}, "_oai": {"id": "oai:soar-ir.repo.nii.ac.jp:00013302", "sets": ["1310"]}, "author_link": ["40285", "40286", "40287", "40288", "40289", "40290", "40291", "40292"], "item_1628147817048": {"attribute_name": "出版タイプ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_ab4af688f83e57aa", "subitem_version_type": "AM"}]}, "item_6_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2013-04", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "4", "bibliographicPageEnd": "365", "bibliographicPageStart": "360", "bibliographicVolumeNumber": "115", "bibliographic_titles": [{"bibliographic_title": "JOURNAL OF BIOSCIENCE AND BIOENGINEERING"}]}]}, "item_6_description_20": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "A full-length cDNA coding for a putative adenosine deaminase (Fv-ada) was isolated from the basidiomycete Flammulina velutipes. Fv-ada encodes a polypeptide consisting of 537 amino acid residues, which has a consensus sequence conserved among adenosine deaminase-related growth factors (ADGF) found in several metazoa, including chordates and insects. Fv-ada transcript was detected at all stages of growth in dikaryotic F. velutipes cells, with a peak at the primordial stage. Heterologous expression of Fv-ada in the yeast Pichia pastoris produced recombinant Fv-ADA that catalyzed the conversion of adenosine to inosine. Dikaryotic mycelia from F. velutipes were transformed with the binary plasmid pFungiway-Fv-ada, which was designed to suppress the expression of Fv-ada through RNA interference. The growth rates of the resulting transformants were retarded in response to the degree of suppression, indicating that Fv-ada plays an important role in the mycelial growth of F. velutipes. 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Fv-ada encodes a polypeptide consisting of 537 amino acid residues, which has a consensus sequence conserved among adenosine deaminase-related growth factors (ADGF) found in several metazoa, including chordates and insects. Fv-ada transcript was detected at all stages of growth in dikaryotic F. velutipes cells, with a peak at the primordial stage. Heterologous expression of Fv-ada in the yeast Pichia pastoris produced recombinant Fv-ADA that catalyzed the conversion of adenosine to inosine. Dikaryotic mycelia from F. velutipes were transformed with the binary plasmid pFungiway-Fv-ada, which was designed to suppress the expression of Fv-ada through RNA interference. The growth rates of the resulting transformants were retarded in response to the degree of suppression, indicating that Fv-ada plays an important role in the mycelial growth of F. velutipes. These results suggested that ADGF could function as growth factors in fungi, as is seen in other eukaryotes. © 2012, The Society for Biotechnology, Japan. All rights reserved."}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Sekiya,  Shuichi"}], "nameIdentifiers": [{"nameIdentifier": "40285", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Yamada,  Masato"}], "nameIdentifiers": [{"nameIdentifier": "40286", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Shibata,  Kou"}], "nameIdentifiers": [{"nameIdentifier": "40287", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Okuhara,  Toru"}], "nameIdentifiers": [{"nameIdentifier": "40288", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Yoshida,  Masumi"}], "nameIdentifiers": [{"nameIdentifier": "40289", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Inatomi,  Satoshi"}], "nameIdentifiers": [{"nameIdentifier": "40290", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Taguchi,  Goro"}], "nameIdentifiers": [{"nameIdentifier": "40291", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Shimosaka,  Makoto"}], "nameIdentifiers": [{"nameIdentifier": "40292", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2015-09-28"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "Characterization_gene_coding_putative_adenosine.pdf", "filesize": [{"value": "1.3 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_note", "mimetype": "application/pdf", "size": 1300000.0, "url": {"label": "Characterization_gene_coding_putative_adenosine.pdf", "url": "https://soar-ir.repo.nii.ac.jp/record/13302/files/Characterization_gene_coding_putative_adenosine.pdf"}, "version_id": "601b5a40-a65d-4f9b-9551-6a65db9d75c1"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "Adenosine deaminase", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Basidiomycete", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Flammulina velutipes", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Growth factor", "subitem_subject_scheme": "Other"}, {"subitem_subject": "RNA interference", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Characterization of a gene coding for a putative adenosine deaminase-related growth factor by RNA interference in the Basidiomycete Flammulina velutipes", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Characterization of a gene coding for a putative adenosine deaminase-related growth factor by RNA interference in the Basidiomycete Flammulina velutipes", "subitem_title_language": "en"}]}, "item_type_id": "6", "owner": "1", "path": ["1310"], "permalink_uri": "http://hdl.handle.net/10091/17843", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2014-09-05"}, "publish_date": "2014-09-05", "publish_status": "0", "recid": "13302", "relation": {}, "relation_version_is_last": true, "title": ["Characterization of a gene coding for a putative adenosine deaminase-related growth factor by RNA interference in the Basidiomycete Flammulina velutipes"], "weko_shared_id": -1}