WEKO3
-
RootNode
アイテム
Isolation of genes coding for chitin-degrading enzymes in the novel chitinolytic bacterium, Chitiniphilus shinanonensis, and characterization of a gene coding for a family 19 chitinase
http://hdl.handle.net/10091/16192
http://hdl.handle.net/10091/16192aad1b2ca-1af6-4626-bc9c-53a743d3ce83
名前 / ファイル | ライセンス | アクション |
---|---|---|
![]() |
|
Item type | 学術雑誌論文 / Journal Article(1) | |||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
公開日 | 2013-01-18 | |||||||||||||||||||
タイトル | ||||||||||||||||||||
タイトル | Isolation of genes coding for chitin-degrading enzymes in the novel chitinolytic bacterium, Chitiniphilus shinanonensis, and characterization of a gene coding for a family 19 chitinase | |||||||||||||||||||
言語 | ||||||||||||||||||||
言語 | eng | |||||||||||||||||||
DOI | ||||||||||||||||||||
関連識別子 | https://doi.org/10.1016/j.jbiosc.2011.10.018 | |||||||||||||||||||
関連名称 | 10.1016/j.jbiosc.2011.10.018 | |||||||||||||||||||
キーワード | ||||||||||||||||||||
主題 | Chitiniphilus shinanonensis, Family 19 chitinase, Chitin-binding domain, Fungal growth inhibition | |||||||||||||||||||
資源タイプ | ||||||||||||||||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||||||||
タイプ | journal article | |||||||||||||||||||
著者 |
Huang, Lanxiang
× Huang, Lanxiang
× Garbulewska, Ewelina
× Sato, Kazuaki
× Kato, Yuichi
× Nogawa, Masahiro
× Taguchi, Goro
× Shimosaka, Makoto
|
|||||||||||||||||||
信州大学研究者総覧へのリンク | ||||||||||||||||||||
氏名 | Nogawa, Masahiro | |||||||||||||||||||
URL | http://soar-rd.shinshu-u.ac.jp/profile/ja.gNcajNnU.html | |||||||||||||||||||
信州大学研究者総覧へのリンク | ||||||||||||||||||||
氏名 | Taguchi, Goro | |||||||||||||||||||
URL | http://soar-rd.shinshu-u.ac.jp/profile/ja.uCDpjekV.html | |||||||||||||||||||
信州大学研究者総覧へのリンク | ||||||||||||||||||||
氏名 | Shimosaka, Makoto | |||||||||||||||||||
URL | http://soar-rd.shinshu-u.ac.jp/profile/ja.ZekmjekV.html | |||||||||||||||||||
出版者 | ||||||||||||||||||||
出版者 | SOC BIOSCIENCE BIOENGINEERING JAPAN | |||||||||||||||||||
引用 | ||||||||||||||||||||
内容記述 | JOURNAL OF BIOSCIENCE AND BIOENGINEERING. 113(3):293-299 (2012) | |||||||||||||||||||
書誌情報 |
JOURNAL OF BIOSCIENCE AND BIOENGINEERING 巻 113, 号 3, p. 293-299, 発行日 2012-03 |
|||||||||||||||||||
抄録 | ||||||||||||||||||||
内容記述 | Chitiniphilus shinanonensis type strain SAY3(T) is a strongly chitinolytic bacterium, originally isolated from the moat water in Ueda, Japan. To elucidate the chitinolytic activity of this strain, 15 genes (chiA-chiO) coding for putative chitin-degrading enzymes were isolated from a genomic library. Sequence analysis revealed the genes comprised 12 family 18 chitinases, a family 19 chitinase, a family 20 beta-N-acetylglucosaminidase, and a polypeptide with a chitin-binding domain but devoid of a catalytic domain. Two operons were detected among the sequences: chiCDEFG and chiLM. The gene coding for the polypeptide (chiN) showed sequence similarity to family 19 chitinases and was successfully expressed in Escherichia colt. ChiN demonstrated a multi-domain structure, composed of the N-terminal, two chitin-binding domains connected by a Pro- and Thr-rich linker, and a family 19 catalytic domain located at the C-terminus. The recombinant protein rChiN catalyzed an endo-type cleavage of N-acetyl-D-glucosamine oligomers, and also degraded insoluble chitin and soluble chitosan (degree of deacetylation of 80%). rChiN exhibited an inhibitory effect on hyphal growth of the fungus Trichoderma reesei. The chitin-binding domains of ChiN likely play an important role in the degradation of insoluble chitin, and are responsible for a growth inhibitory effect on fungi. (C) 2011, The Society for Biotechnology, Japan. All rights reserved. | |||||||||||||||||||
資源タイプ(コンテンツの種類) | ||||||||||||||||||||
ISSN | ||||||||||||||||||||
収録物識別子タイプ | ISSN | |||||||||||||||||||
収録物識別子 | 1389-1723 | |||||||||||||||||||
書誌レコードID | ||||||||||||||||||||
収録物識別子タイプ | NCID | |||||||||||||||||||
収録物識別子 | AA11307678 | |||||||||||||||||||
PubMed | ||||||||||||||||||||
識別子タイプ | PMID | |||||||||||||||||||
関連識別子 | http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?CMD=search&DB=pubmed&term=22178339 | |||||||||||||||||||
関連名称 | 22178339 | |||||||||||||||||||
権利 | ||||||||||||||||||||
権利情報 | Copyright© 2011, The Society for Biotechnology, Japan. | |||||||||||||||||||
出版タイプ | ||||||||||||||||||||
出版タイプ | AM | |||||||||||||||||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||||||||||||||||
WoS | ||||||||||||||||||||
URL | http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=ShinshuUniv&SrcApp=ShinshuUniv&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000302505700004 |