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Stimulatory effect of Rho-associated coiled-coil kinase (ROCK) inhibitor on revivability of in vitro-produced bovine blastocysts after vitrification
http://hdl.handle.net/10091/16149
http://hdl.handle.net/10091/16149ed3d5093-1f67-4b03-b1e2-494ffbed0de8
| 名前 / ファイル | ライセンス | アクション |
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THE73_8_1139.pdf (129.2 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||||||||||||||||
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| 公開日 | 2012-12-19 | |||||||||||||||||||
| タイトル | ||||||||||||||||||||
| タイトル | Stimulatory effect of Rho-associated coiled-coil kinase (ROCK) inhibitor on revivability of in vitro-produced bovine blastocysts after vitrification | |||||||||||||||||||
| 言語 | ||||||||||||||||||||
| 言語 | eng | |||||||||||||||||||
| DOI | ||||||||||||||||||||
| 関連識別子 | https://doi.org/10.1016/j.theriogenology.2010.01.015 | |||||||||||||||||||
| 関連名称 | 10.1016/j.theriogenology.2010.01.015 | |||||||||||||||||||
| キーワード | ||||||||||||||||||||
| 主題 | Bovine blastocysts, IVF, Rho-associated coiled-coil kinase, Vitrification, Y-27632 | |||||||||||||||||||
| 資源タイプ | ||||||||||||||||||||
| 資源 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||||||||
| タイプ | journal article | |||||||||||||||||||
| 著者 |
Hochi, S
× Hochi, S
× Abdalla, H
× Hara, H
× Shimoda, M
× Morita, H
× Kuwayama, M
× Hirabayashi, M
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| 信州大学研究者総覧へのリンク | ||||||||||||||||||||
| 氏名 | Hochi, S | |||||||||||||||||||
| URL | http://soar-rd.shinshu-u.ac.jp/profile/ja.HhfNjekV.html | |||||||||||||||||||
| 出版者 | ||||||||||||||||||||
| 出版者 | ELSEVIER SCIENCE INC | |||||||||||||||||||
| 引用 | ||||||||||||||||||||
| 内容記述 | THERIOGENOLOGY. 73(8):1139-1145 (2010) | |||||||||||||||||||
| 書誌情報 |
THERIOGENOLOGY 巻 73, 号 8, p. 1139-1145, 発行日 2010-05 |
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| 抄録 | ||||||||||||||||||||
| 内容記述 | Inhibition of Rho-associated kinase (ROCK) activity promoted recovery and growth of frozen-thawed human embryonic stem cells. The primary objective was to determine if a ROCK inhibitor (Y-27632) in post-thaw culture medium unproved revivability of vitrified IVP bovine blastocysts. Expanding or expanded blastocysts (7 d after IVF) were vitrified (minimum volume cooling procedure, using a Cryotop) in 15% ethylene glycol. 15% DMSO and 0 5 M sucrose When post-warm blastocysts were cultured in inSOF medium, survival rate (re-expansion of blastocoel at 24 h of culture) was improved (P < 0 05) by the addition of 10 mu M Y-27632 (94 9 +/- 2 4%, mean +/- SEM) compared to a control (78 0 +/- 6 0%) Conversely. after 48 h of culture, there were no significant differences in hatching late (62.8 +/- 11 1 vs 59 6 +/- 9.4%) and mean total cell number (135 2 +/- 13 1 vs. 146 7 +/- 13 3) In non-vitrified IVP bovine blastocysts, the hatching rate on Day 9 was improved by Y-27632 (91 7 +/- 3 8 vs 54 7 +/- 8 9%. P < 0 05). with no difference in mean total cell number of blastocysts (230 0 +/- 23 0 vs 191 2 +/- 22 2, P = 0 23). In an additional experiment, Y-27632 was added to culture medium on either Day 0, Day 2. or Day 4 (and remained present until Day 8). resulting in no improvement in blastocyst yield compared to a control group (7.5 +/- 2 1, 31 4 +/- 2 3, 36 2 +/- 3.2. and 28.6 +/- 6.9%. respectively) In conclusion, adding a ROCK inhibitor to post-thaw culture medium improved revivability of IVP bovine blastocysts after vitrification and warming | |||||||||||||||||||
| 資源タイプ（コンテンツの種類） | ||||||||||||||||||||
| ISSN | ||||||||||||||||||||
| 収録物識別子タイプ | ISSN | |||||||||||||||||||
| 収録物識別子 | 0093-691X | |||||||||||||||||||
| 書誌レコードID | ||||||||||||||||||||
| 収録物識別子タイプ | NCID | |||||||||||||||||||
| 収録物識別子 | AA00862972 | |||||||||||||||||||
| PubMed | ||||||||||||||||||||
| 識別子タイプ | PMID | |||||||||||||||||||
| 関連識別子 | http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?CMD=search&DB=pubmed&term=20171722 | |||||||||||||||||||
| 関連名称 | 20171722 | |||||||||||||||||||
| 出版タイプ | ||||||||||||||||||||
| 出版タイプ | AM | |||||||||||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||||||||||||||||
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| URL | http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=ShinshuUniv&SrcApp=ShinshuUniv&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000276801400016 | |||||||||||||||||||
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Hochi, S, Abdalla, H, Hara, H, Shimoda, M, Morita, H, Kuwayama, M, Hirabayashi, M, 2010, Stimulatory effect of Rho-associated coiled-coil kinase (ROCK) inhibitor on revivability of in vitro-produced bovine blastocysts after vitrification: ELSEVIER SCIENCE INC, 1139–1145 p.
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