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The primary objective was to determine if a ROCK inhibitor (Y-27632) in post-thaw culture medium unproved revivability of vitrified IVP bovine blastocysts. Expanding or expanded blastocysts (7 d after IVF) were vitrified (minimum volume cooling procedure, using a Cryotop) in 15% ethylene glycol. 15% DMSO and 0 5 M sucrose When post-warm blastocysts were cultured in inSOF medium, survival rate (re-expansion of blastocoel at 24 h of culture) was improved (P \u003c 0 05) by the addition of 10 mu M Y-27632 (94 9 +/- 2 4%, mean +/- SEM) compared to a control (78 0 +/- 6 0%) Conversely. after 48 h of culture, there were no significant differences in hatching late (62.8 +/- 11 1 vs 59 6 +/- 9.4%) and mean total cell number (135 2 +/- 13 1 vs. 146 7 +/- 13 3) In non-vitrified IVP bovine blastocysts, the hatching rate on Day 9 was improved by Y-27632 (91 7 +/- 3 8 vs 54 7 +/- 8 9%. 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  1. 080 繊維学部
  2. 0801 学術論文

Stimulatory effect of Rho-associated coiled-coil kinase (ROCK) inhibitor on revivability of in vitro-produced bovine blastocysts after vitrification

http://hdl.handle.net/10091/16149
ed3d5093-1f67-4b03-b1e2-494ffbed0de8
名前 / ファイル ライセンス アクション
THE73_8_1139.pdf THE73_8_1139.pdf (129.2 kB)
Item type 学術雑誌論文 / Journal Article(1)
公開日 2012-12-19
タイトル
言語 en
タイトル Stimulatory effect of Rho-associated coiled-coil kinase (ROCK) inhibitor on revivability of in vitro-produced bovine blastocysts after vitrification
言語
言語 eng
キーワード
主題Scheme Other
主題 Bovine blastocysts
キーワード
主題Scheme Other
主題 IVF
キーワード
主題Scheme Other
主題 Rho-associated coiled-coil kinase
キーワード
主題Scheme Other
主題 Vitrification
キーワード
主題Scheme Other
主題 Y-27632
資源タイプ
資源 http://purl.org/coar/resource_type/c_6501
タイプ journal article
著者 Hochi, S

× Hochi, S

WEKO 40794

Hochi, S

Search repository
Abdalla, H

× Abdalla, H

WEKO 40795

Abdalla, H

Search repository
Hara, H

× Hara, H

WEKO 40796

Hara, H

Search repository
Shimoda, M

× Shimoda, M

WEKO 40797

Shimoda, M

Search repository
Morita, H

× Morita, H

WEKO 40798

Morita, H

Search repository
Kuwayama, M

× Kuwayama, M

WEKO 40799

Kuwayama, M

Search repository
Hirabayashi, M

× Hirabayashi, M

WEKO 40800

Hirabayashi, M

Search repository
信州大学研究者総覧へのリンク
氏名 Hochi, S
URL http://soar-rd.shinshu-u.ac.jp/profile/ja.HhfNjekV.html
出版者
出版者 ELSEVIER SCIENCE INC
引用
内容記述タイプ Other
内容記述 THERIOGENOLOGY. 73(8):1139-1145 (2010)
書誌情報 THERIOGENOLOGY

巻 73, 号 8, p. 1139-1145, 発行日 2010-05
抄録
内容記述タイプ Abstract
内容記述 Inhibition of Rho-associated kinase (ROCK) activity promoted recovery and growth of frozen-thawed human embryonic stem cells. The primary objective was to determine if a ROCK inhibitor (Y-27632) in post-thaw culture medium unproved revivability of vitrified IVP bovine blastocysts. Expanding or expanded blastocysts (7 d after IVF) were vitrified (minimum volume cooling procedure, using a Cryotop) in 15% ethylene glycol. 15% DMSO and 0 5 M sucrose When post-warm blastocysts were cultured in inSOF medium, survival rate (re-expansion of blastocoel at 24 h of culture) was improved (P < 0 05) by the addition of 10 mu M Y-27632 (94 9 +/- 2 4%, mean +/- SEM) compared to a control (78 0 +/- 6 0%) Conversely. after 48 h of culture, there were no significant differences in hatching late (62.8 +/- 11 1 vs 59 6 +/- 9.4%) and mean total cell number (135 2 +/- 13 1 vs. 146 7 +/- 13 3) In non-vitrified IVP bovine blastocysts, the hatching rate on Day 9 was improved by Y-27632 (91 7 +/- 3 8 vs 54 7 +/- 8 9%. P < 0 05). with no difference in mean total cell number of blastocysts (230 0 +/- 23 0 vs 191 2 +/- 22 2, P = 0 23). In an additional experiment, Y-27632 was added to culture medium on either Day 0, Day 2. or Day 4 (and remained present until Day 8). resulting in no improvement in blastocyst yield compared to a control group (7.5 +/- 2 1, 31 4 +/- 2 3, 36 2 +/- 3.2. and 28.6 +/- 6.9%. respectively) In conclusion, adding a ROCK inhibitor to post-thaw culture medium improved revivability of IVP bovine blastocysts after vitrification and warming
資源タイプ(コンテンツの種類)
内容記述タイプ Other
内容記述 Article
ISSN
収録物識別子タイプ ISSN
収録物識別子 0093-691X
書誌レコードID
収録物識別子タイプ NCID
収録物識別子 AA00862972
PubMed
関連識別子
識別子タイプ PMID
関連識別子 http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?CMD=search&DB=pubmed&term=20171722
関連名称
関連名称 20171722
DOI
関連識別子
識別子タイプ DOI
関連識別子 https://doi.org/10.1016/j.theriogenology.2010.01.015
関連名称
関連名称 10.1016/j.theriogenology.2010.01.015
出版タイプ
出版タイプ AM
出版タイプResource http://purl.org/coar/version/c_ab4af688f83e57aa
WoS
表示名 Web of Science
URL http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=ShinshuUniv&SrcApp=ShinshuUniv&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000276801400016
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