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Purification and characterization of UDP-glucose: hydroxycoumarin 7-O-glucosyltransferase, with broad substrate specificity from tobacco cultured cells
http://hdl.handle.net/10091/10111
http://hdl.handle.net/10091/10111e35b0c63-0900-41b0-a55f-d66da91e5c43
名前 / ファイル | ライセンス | アクション |
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Plant Sci 2000.pdf (1.3 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2010-05-26 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Purification and characterization of UDP-glucose: hydroxycoumarin 7-O-glucosyltransferase, with broad substrate specificity from tobacco cultured cells | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題 | Nicotiana tabacum L. cv Bright Yellow, glucosyltransferase, scopoletin, esculetin, flavonoid | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | journal article | |||||
著者 |
Taguchi, G
× Taguchi, G× Imura, H× Maeda, Y× Kodaira, R× Hayashida, N× Shimosaka, M× Okazaki, M |
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信州大学研究者総覧へのリンク | ||||||
氏名 | Taguchi, G | |||||
URL | http://soar-rd.shinshu-u.ac.jp/profile/ja.uCDpjekV.html | |||||
信州大学研究者総覧へのリンク | ||||||
氏名 | Hayashida, N | |||||
URL | http://soar-rd.shinshu-u.ac.jp/profile/ja.HVfmjekV.html | |||||
信州大学研究者総覧へのリンク | ||||||
氏名 | Shimosaka, M | |||||
URL | http://soar-rd.shinshu-u.ac.jp/profile/ja.ZekmjekV.html | |||||
出版者 | ||||||
出版者 | Elsevier | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Plant Science. 157(1):105-112 (2000) | |||||
書誌情報 |
Plant Science 巻 157, 号 1, p. 105-112, 発行日 2000 |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | The enzyme UDP-glucose: hydroxycoumarin 7-O-glucosyltransferase (CGTase), which catalyzes the formation of scopolin from scopoletin, was purified approximately 1200-fold from a culture of 2,4-D-treated tobacco cells (Nicotiana tabacum L. cv. Bright Yellow T-13) with a yield of 7%. Purification to apparent homogeneity, as judged by SDS-PAGE, was achieved by sequential anion-exchange chromatography, hydroxyapatite chromatography, gel filtration, a second round of anion-exchange chromatography, and affinity chromatography on UDP-glucuronic acid agarose. The purified enzyme had a pH optimum of 7.5, an isoelectric point (pI) of 5.0, and a molecular mass of 49 kDa. The enzyme did not require metal cofactors for activity. Its activity was inhibited by Zn2+, Co2+ and Cu2+ ions, as well as by SH-blocking reagents. The K-m values for UDP-glucose, scopoletin and esculetin were 43, 150 and 25 mu M. respectively. A study of the initial rate of the reaction suggested that the reaction proceeded via a sequential mechanism. The purified enzyme preferred hydroxycoumarins as substrates but also exhibited significant activity with flavonoids. A database search using the amino terminus amino acid sequence of CGTase revealed strong homology to the amino acid sequences of other glucosyltransferases in plants. | |||||
資源タイプ(コンテンツの種類) | ||||||
内容記述タイプ | Other | |||||
内容記述 | Article | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0168-9452 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA10531279 | |||||
他の資源との関係:URI | ||||||
識別子タイプ | URI | |||||
関連識別子 | http://www.elsevier.com/locate/plantsci | |||||
関連名称 | http://www.elsevier.com/locate/plantsci | |||||
PubMed | ||||||
識別子タイプ | PMID | |||||
関連識別子 | http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?CMD=search&DB=pubmed&term=10940474 | |||||
関連名称 | 10940474 | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | https://doi.org/10.1016/S0168-9452(00)00270-3 | |||||
関連名称 | 10.1016/S0168-9452(00)00270-3 | |||||
出版タイプ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
WoS | ||||||
表示名 | Web of Science | |||||
URL | http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=ShinshuUniv&SrcApp=ShinshuUniv&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000088553500011 |