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Enrichments of gene replacement events by Agrobacterium-mediated recombinase-mediated cassette exchange
http://hdl.handle.net/10091/00018680
http://hdl.handle.net/10091/00018680fbbde469-34e3-4b23-a3f9-e434f21a1992
| 名前 / ファイル | ライセンス | アクション |
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Enrichments_of_gene_replacement_events.pdf (431.1 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||||||||
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| 公開日 | 2016-03-23 | |||||||||||
| タイトル | ||||||||||||
| タイトル | Enrichments of gene replacement events by Agrobacterium-mediated recombinase-mediated cassette exchange | |||||||||||
| 言語 | ||||||||||||
| 言語 | eng | |||||||||||
| DOI | ||||||||||||
| 関連識別子 | https://doi.org/10.1007/s11032-015-0215-7 | |||||||||||
| 関連名称 | 10.1007/s11032-015-0215-7 | |||||||||||
| キーワード | ||||||||||||
| 主題 | RMCE, Gene replacement, Negative selection | |||||||||||
| 資源タイプ | ||||||||||||
| 資源 | http://purl.org/coar/resource_type/c_6501 | |||||||||||
| タイプ | journal article | |||||||||||
| 著者 |
Ebinuma, Hiroyasu
× Ebinuma, Hiroyasu
× Nakahama, Katsuhiko
× Nanto, Kazuya
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| 信州大学研究者総覧へのリンク | ||||||||||||
| 氏名 | Ebinuma, Hiroyasu | |||||||||||
| URL | http://soar-rd.shinshu-u.ac.jp/profile/ja.gechjFkV.html | |||||||||||
| 出版者 | ||||||||||||
| 出版者 | SPRINGER | |||||||||||
| 引用 | ||||||||||||
| 内容記述 | MOLECULAR BREEDING. 35(2):82 (2015) | |||||||||||
| 書誌情報 |
MOLECULAR BREEDING 巻 35, 号 2, p. 82, 発行日 2015-02 |
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| 抄録 | ||||||||||||
| 内容記述 | We report recombinase-mediated cassette exchange (RMCE), which can permit integration of transgenes into pre-defined chromosomal loci with no co-expressed marker gene by using Agrobacterium-mediated transformation. Transgenic tobacco plants which have a single copy of negative marker genes (codA) at target loci in heterozygous and homozygous conditions were used for gene exchange by the RMCE method. By negative selection, we were able to obtain five heterozygous and four homozygous transgenic plants in which the genes were exchanged from 64 leaf segments of heterozygous and homozygous target plants, respectively. Except for one transgenic plant with an extra copy, the other eight plants had only a single copy of marker-free transgenes, and no footprint of random integrated copies was detected in half of the eight plants. The RMCE re-transformation frequencies were calculated as 6.25 % per explant and were approximately the same as the average percentage of intact single-copy transformation events for standard tobacco Agrobacterium-mediated transformation. | |||||||||||
| 資源タイプ（コンテンツの種類） | ||||||||||||
| ISSN | ||||||||||||
| 収録物識別子タイプ | ISSN | |||||||||||
| 収録物識別子 | 1380-3743 | |||||||||||
| 書誌レコードID | ||||||||||||
| 収録物識別子タイプ | NCID | |||||||||||
| 収録物識別子 | AA1104032X | |||||||||||
| 権利 | ||||||||||||
| 権利情報 | © The Author(s) 2015 Open Access This article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited. | |||||||||||
| 出版タイプ | ||||||||||||
| 出版タイプ | VoR | |||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||
| WoS | ||||||||||||
| URL | http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=ShinshuUniv&SrcApp=ShinshuUniv&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000349765300026 | |||||||||||
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Cite as
Ebinuma, Hiroyasu, Nakahama, Katsuhiko, Nanto, Kazuya, 2015, Enrichments of gene replacement events by Agrobacterium-mediated recombinase-mediated cassette exchange: SPRINGER, 82– p.
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