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  1. 080 繊維学部
  2. 0801 学術論文

High revivability of vitrified-warmed bovine mature oocytes after recovery culture with alpha-tocopherol

http://hdl.handle.net/10091/00018693
http://hdl.handle.net/10091/00018693
2bdfd5dd-4a81-4e5d-91e1-78948aafcea1
名前 / ファイル ライセンス アクション
High_revivability_of_vitrified-warmed.pdf High_revivability_of_vitrified-warmed.pdf (336.9 kB)
Item type 学術雑誌論文 / Journal Article(1)
公開日 2016-03-23
タイトル
言語 en
タイトル High revivability of vitrified-warmed bovine mature oocytes after recovery culture with alpha-tocopherol
言語
言語 eng
キーワード
主題Scheme Other
主題 IN-VITRO FERTILIZATION
キーワード
主題Scheme Other
主題 HIGH DEVELOPMENTAL RATES
キーワード
主題Scheme Other
主題 CELL NUCLEAR TRANSFER
キーワード
主題Scheme Other
主題 L-ASCORBIC-ACID
キーワード
主題Scheme Other
主題 MOUSE OOCYTES
キーワード
主題Scheme Other
主題 MAMMALIAN FERTILIZATION
キーワード
主題Scheme Other
主題 PORCINE EMBRYOS
キーワード
主題Scheme Other
主題 CRYO-TOLERANCE
キーワード
主題Scheme Other
主題 L-CARNITINE
キーワード
主題Scheme Other
主題 VITRIFICATION
資源タイプ
資源 http://purl.org/coar/resource_type/c_6501
タイプ journal article
著者 Yashiro, Ikuko

× Yashiro, Ikuko

WEKO 50071

Yashiro, Ikuko

Search repository
Tagiri, Miho

× Tagiri, Miho

WEKO 50072

Tagiri, Miho

Search repository
Ogawa, Hayato

× Ogawa, Hayato

WEKO 50073

Ogawa, Hayato

Search repository
Tashima, Kazuya

× Tashima, Kazuya

WEKO 50074

Tashima, Kazuya

Search repository
Takashima, Seiji

× Takashima, Seiji

WEKO 50075

Takashima, Seiji

Search repository
Hara, Hiromasa

× Hara, Hiromasa

WEKO 50076

Hara, Hiromasa

Search repository
Hirabayashi, Masumi

× Hirabayashi, Masumi

WEKO 50077

Hirabayashi, Masumi

Search repository
Hochi, Shinichi

× Hochi, Shinichi

WEKO 50078

Hochi, Shinichi

Search repository
信州大学研究者総覧へのリンク
氏名 Takashima, Seiji
URL http://soar-rd.shinshu-u.ac.jp/profile/ja.jUyUyayV.html
信州大学研究者総覧へのリンク
氏名 Hochi, Shinichi
URL http://soar-rd.shinshu-u.ac.jp/profile/ja.HhfNjekV.html
出版者
出版者 BIOSCIENTIFICA LTD
引用
内容記述タイプ Other
内容記述 REPRODUCTION. 149(4):347-355 (2015)
書誌情報 REPRODUCTION

巻 149, 号 4, p. 347-355, 発行日 2015-04
内容記述
内容記述タイプ Other
内容記述 online publication: 27 January 2015
抄録
内容記述タイプ Abstract
内容記述 The objective of this study was to investigate whether developmental competence of vitrified–warmed bovine oocytes can be improved by antioxidant treatment during recovery culture. In experiment 1, one of the two antioxidants (either l-ascorbic acid or α-tocopherol) was added as a supplement to the recovery culture medium to which postwarming oocytes were exposed for 2 h before IVF. The exposure to α-tocopherol had a positive effect on rescuing the oocytes as assessed by the blastocyst yield 8 days after the IVF (35.1–36.3% vs 19.2–25.8% in untreated postwarming oocytes). Quality of expanding blastocysts harvested on Day 8 was comparable between α-tocopherol-treated vitrification group and fresh control group in terms of total cell number and chromosomal ploidy. In experiment 2, level of reactive oxygen species, mitochondrial activity, and distribution of cortical granules in α-tocopherol-treated postwarming oocytes were assessed. No obvious differences from the control data were found in these parameters. However, the treatment with α-tocopherol increased the percentage of zygotes exhibiting normal single aster formation (90.3% vs 48.0% in untreated postwarming oocytes; 10 h post-IVF). It was concluded that α-tocopherol treatment of vitrified–warmed bovine mature oocytes during recovery culture can improve their revivability, as shown by the high blastocyst yield and the higher mean total cell number in the blastocysts.
資源タイプ(コンテンツの種類)
内容記述タイプ Other
内容記述 Article
ISSN
収録物識別子タイプ ISSN
収録物識別子 1470-1626
書誌レコードID
収録物識別子タイプ NCID
収録物識別子 AA11501251
PubMed
識別子タイプ PMID
関連識別子 http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?CMD=search&DB=pubmed&term=25628440
関連名称 25628440
DOI
識別子タイプ DOI
関連識別子 https://doi.org/10.1530/REP-14-0594
関連名称 10.1530/REP-14-0594
権利
権利情報 This is not the definitive version of record of this article.This manuscript has been accepted for publication in REPRODUCTION, but the version presented here has not yet been copy-edited, formatted or proofed. Consequently, Bioscientifica accepts no responsibility for any errors or omissions it may contain. The definitive version is now freely available at https://doi.org/10.1530/REP-14-0594 2015.
出版タイプ
出版タイプ AM
出版タイプResource http://purl.org/coar/version/c_ab4af688f83e57aa
WoS
表示名 Web of Science
URL http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=ShinshuUniv&SrcApp=ShinshuUniv&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000351919100009
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