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High revivability of vitrified-warmed bovine mature oocytes after recovery culture with alpha-tocopherol
http://hdl.handle.net/10091/00018693
http://hdl.handle.net/10091/000186932bdfd5dd-4a81-4e5d-91e1-78948aafcea1
| 名前 / ファイル | ライセンス | アクション |
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High_revivability_of_vitrified-warmed.pdf (336.9 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||||||||||||||||||
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| 公開日 | 2016-03-23 | |||||||||||||||||||||
| タイトル | ||||||||||||||||||||||
| タイトル | High revivability of vitrified-warmed bovine mature oocytes after recovery culture with alpha-tocopherol | |||||||||||||||||||||
| 言語 | ||||||||||||||||||||||
| 言語 | eng | |||||||||||||||||||||
| DOI | ||||||||||||||||||||||
| 関連識別子 | https://doi.org/10.1530/REP-14-0594 | |||||||||||||||||||||
| 関連名称 | 10.1530/REP-14-0594 | |||||||||||||||||||||
| キーワード | ||||||||||||||||||||||
| 主題 | IN-VITRO FERTILIZATION, HIGH DEVELOPMENTAL RATES, CELL NUCLEAR TRANSFER, L-ASCORBIC-ACID, MOUSE OOCYTES, MAMMALIAN FERTILIZATION, PORCINE EMBRYOS, CRYO-TOLERANCE, L-CARNITINE, VITRIFICATION | |||||||||||||||||||||
| 資源タイプ | ||||||||||||||||||||||
| 資源 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||||||||||
| タイプ | journal article | |||||||||||||||||||||
| 著者 |
Yashiro, Ikuko
× Yashiro, Ikuko
× Tagiri, Miho
× Ogawa, Hayato
× Tashima, Kazuya
× Takashima, Seiji
× Hara, Hiromasa
× Hirabayashi, Masumi
× Hochi, Shinichi
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| 信州大学研究者総覧へのリンク | ||||||||||||||||||||||
| 氏名 | Takashima, Seiji | |||||||||||||||||||||
| URL | http://soar-rd.shinshu-u.ac.jp/profile/ja.jUyUyayV.html | |||||||||||||||||||||
| 信州大学研究者総覧へのリンク | ||||||||||||||||||||||
| 氏名 | Hochi, Shinichi | |||||||||||||||||||||
| URL | http://soar-rd.shinshu-u.ac.jp/profile/ja.HhfNjekV.html | |||||||||||||||||||||
| 出版者 | ||||||||||||||||||||||
| 出版者 | BIOSCIENTIFICA LTD | |||||||||||||||||||||
| 引用 | ||||||||||||||||||||||
| 内容記述 | REPRODUCTION. 149(4):347-355 (2015) | |||||||||||||||||||||
| 書誌情報 |
REPRODUCTION 巻 149, 号 4, p. 347-355, 発行日 2015-04 |
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| 内容記述 | ||||||||||||||||||||||
| 内容記述タイプ | Other | |||||||||||||||||||||
| 内容記述 | online publication: 27 January 2015 | |||||||||||||||||||||
| 抄録 | ||||||||||||||||||||||
| 内容記述 | The objective of this study was to investigate whether developmental competence of vitrified–warmed bovine oocytes can be improved by antioxidant treatment during recovery culture. In experiment 1, one of the two antioxidants (either l-ascorbic acid or α-tocopherol) was added as a supplement to the recovery culture medium to which postwarming oocytes were exposed for 2 h before IVF. The exposure to α-tocopherol had a positive effect on rescuing the oocytes as assessed by the blastocyst yield 8 days after the IVF (35.1–36.3% vs 19.2–25.8% in untreated postwarming oocytes). Quality of expanding blastocysts harvested on Day 8 was comparable between α-tocopherol-treated vitrification group and fresh control group in terms of total cell number and chromosomal ploidy. In experiment 2, level of reactive oxygen species, mitochondrial activity, and distribution of cortical granules in α-tocopherol-treated postwarming oocytes were assessed. No obvious differences from the control data were found in these parameters. However, the treatment with α-tocopherol increased the percentage of zygotes exhibiting normal single aster formation (90.3% vs 48.0% in untreated postwarming oocytes; 10 h post-IVF). It was concluded that α-tocopherol treatment of vitrified–warmed bovine mature oocytes during recovery culture can improve their revivability, as shown by the high blastocyst yield and the higher mean total cell number in the blastocysts. | |||||||||||||||||||||
| 資源タイプ（コンテンツの種類） | ||||||||||||||||||||||
| ISSN | ||||||||||||||||||||||
| 収録物識別子タイプ | ISSN | |||||||||||||||||||||
| 収録物識別子 | 1470-1626 | |||||||||||||||||||||
| 書誌レコードID | ||||||||||||||||||||||
| 収録物識別子タイプ | NCID | |||||||||||||||||||||
| 収録物識別子 | AA11501251 | |||||||||||||||||||||
| PubMed | ||||||||||||||||||||||
| 識別子タイプ | PMID | |||||||||||||||||||||
| 関連識別子 | http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?CMD=search&DB=pubmed&term=25628440 | |||||||||||||||||||||
| 関連名称 | 25628440 | |||||||||||||||||||||
| 権利 | ||||||||||||||||||||||
| 権利情報 | This is not the definitive version of record of this article.This manuscript has been accepted for publication in REPRODUCTION, but the version presented here has not yet been copy-edited, formatted or proofed. Consequently, Bioscientifica accepts no responsibility for any errors or omissions it may contain. The definitive version is now freely available at https://doi.org/10.1530/REP-14-0594 2015. | |||||||||||||||||||||
| 出版タイプ | ||||||||||||||||||||||
| 出版タイプ | AM | |||||||||||||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||||||||||||||||||
| WoS | ||||||||||||||||||||||
| URL | http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=ShinshuUniv&SrcApp=ShinshuUniv&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000351919100009 | |||||||||||||||||||||
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Yashiro, Ikuko, Tagiri, Miho, Ogawa, Hayato, Tashima, Kazuya, Takashima, Seiji, Hara, Hiromasa, Hirabayashi, Masumi, Hochi, Shinichi, 2015, High revivability of vitrified-warmed bovine mature oocytes after recovery culture with alpha-tocopherol: BIOSCIENTIFICA LTD, 347–355 p.
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