{"_buckets": {"deposit": "e3dd35a0-01fc-473b-a719-813e65ec75e9"}, "_deposit": {"id": "17925", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "17925"}, "status": "published"}, "_oai": {"id": "oai:soar-ir.repo.nii.ac.jp:00017925"}, "item_6_biblio_info_6": {"attribute_name": "\u66f8\u8a8c\u60c5\u5831", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2015-04", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "4", "bibliographicPageEnd": "355", "bibliographicPageStart": "347", "bibliographicVolumeNumber": "149", "bibliographic_titles": [{"bibliographic_title": "REPRODUCTION"}]}]}, "item_6_description_19": {"attribute_name": "\u5185\u5bb9\u8a18\u8ff0", "attribute_value_mlt": [{"subitem_description": "online publication: 27 January 2015", "subitem_description_type": "Other"}]}, "item_6_description_20": {"attribute_name": "\u6284\u9332", "attribute_value_mlt": [{"subitem_description": "The objective of this study was to investigate whether developmental competence of vitrified\u2013warmed bovine oocytes can be improved by antioxidant treatment during recovery culture. In experiment 1, one of the two antioxidants (either l-ascorbic acid or \u03b1-tocopherol) was added as a supplement to the recovery culture medium to which postwarming oocytes were exposed for 2\u200ah before IVF. The exposure to \u03b1-tocopherol had a positive effect on rescuing the oocytes as assessed by the blastocyst yield 8 days after the IVF (35.1\u201336.3% vs 19.2\u201325.8% in untreated postwarming oocytes). Quality of expanding blastocysts harvested on Day 8 was comparable between \u03b1-tocopherol-treated vitrification group and fresh control group in terms of total cell number and chromosomal ploidy. In experiment 2, level of reactive oxygen species, mitochondrial activity, and distribution of cortical granules in \u03b1-tocopherol-treated postwarming oocytes were assessed. No obvious differences from the control data were found in these parameters. However, the treatment with \u03b1-tocopherol increased the percentage of zygotes exhibiting normal single aster formation (90.3% vs 48.0% in untreated postwarming oocytes; 10\u200ah post-IVF). It was concluded that \u03b1-tocopherol treatment of vitrified\u2013warmed bovine mature oocytes during recovery culture can improve their revivability, as shown by the high blastocyst yield and the higher mean total cell number in the blastocysts.", "subitem_description_type": "Abstract"}]}, "item_6_description_30": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7\uff08\u30b3\u30f3\u30c6\u30f3\u30c4\u306e\u7a2e\u985e\uff09", "attribute_value_mlt": [{"subitem_description": "Article", "subitem_description_type": "Other"}]}, "item_6_description_5": {"attribute_name": "\u5f15\u7528", "attribute_value_mlt": [{"subitem_description": "REPRODUCTION. 149(4):347-355 (2015)", "subitem_description_type": "Other"}]}, "item_6_link_3": {"attribute_name": "\u4fe1\u5dde\u5927\u5b66\u7814\u7a76\u8005\u7dcf\u89a7\u3078\u306e\u30ea\u30f3\u30af", "attribute_value_mlt": [{"subitem_link_text": "Takashima, Seiji", "subitem_link_url": "http://soar-rd.shinshu-u.ac.jp/profile/ja.jUyUyayV.html"}, {"subitem_link_text": "Hochi, Shinichi", "subitem_link_url": "http://soar-rd.shinshu-u.ac.jp/profile/ja.HhfNjekV.html"}]}, "item_6_link_67": {"attribute_name": "WoS", "attribute_value_mlt": [{"subitem_link_text": "Web of Science", "subitem_link_url": "http://gateway.isiknowledge.com/gateway/Gateway.cgi?\u0026GWVersion=2\u0026SrcAuth=ShinshuUniv\u0026SrcApp=ShinshuUniv\u0026DestLinkType=FullRecord\u0026DestApp=WOS\u0026KeyUT=000351919100009"}]}, "item_6_publisher_4": {"attribute_name": "\u51fa\u7248\u8005", "attribute_value_mlt": [{"subitem_publisher": "BIOSCIENTIFICA LTD"}]}, "item_6_relation_47": {"attribute_name": "PubMed", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "25628440"}], "subitem_relation_type_id": {"subitem_relation_type_id_text": "http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?CMD=search\u0026DB=pubmed\u0026term=25628440", "subitem_relation_type_select": "PMID"}}]}, "item_6_relation_48": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "10.1530/REP-14-0594"}], "subitem_relation_type_id": {"subitem_relation_type_id_text": "http://dx.doi.org/10.1530/REP-14-0594", "subitem_relation_type_select": "DOI"}}]}, "item_6_rights_62": {"attribute_name": "\u6a29\u5229", "attribute_value_mlt": [{"subitem_rights": "This is not the definitive version of record of this article.This manuscript has been accepted for publication in REPRODUCTION, but the version presented here has not yet been copy-edited, formatted or proofed. Consequently, Bioscientifica accepts no responsibility for any errors or omissions it may contain. 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In experiment 1, one of the two antioxidants (either L-ascorbic acid or alpha-tocopherol) was added as a supplement to the recovery culture medium to which postwarming oocytes were exposed for 2 h before IVF. The exposure to a-tocopherol had a positive effect on rescuing the oocytes as assessed by the blastocyst yield 8 days after the IVF (35.1-36.3% vs 19.2-25.8% in untreated postwarming oocytes). Quality of expanding blastocysts harvested on Day 8 was comparable between alpha-tocopherol-treated vitrification group and fresh control group in terms of total cell number and chromosomal ploidy. In experiment 2, level of reactive oxygen species, mitochondrial activity, and distribution of cortical granules in a-tocopherol-treated postwarming oocytes were assessed. No obvious differences from the control data were found in these parameters. However, the treatment with alpha-tocopherol increased the percentage of zygotes exhibiting normal single aster formation (90.3% vs 48.0% in untreated postwarming oocytes; 10 h post-IVF). It was concluded that alpha-tocopherol treatment of vitrified-warmed bovine mature oocytes during recovery culture can improve their revivability, as shown by the high blastocyst yield and the higher mean total cell number in the blastocysts."}]}, "item_creator": {"attribute_name": "\u8457\u8005", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Yashiro,  Ikuko"}], "nameIdentifiers": [{"nameIdentifier": "50071", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Tagiri,  Miho"}], "nameIdentifiers": [{"nameIdentifier": "50072", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Ogawa,  Hayato"}], "nameIdentifiers": [{"nameIdentifier": "50073", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Tashima,  Kazuya"}], "nameIdentifiers": [{"nameIdentifier": "50074", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Takashima,  Seiji"}], "nameIdentifiers": [{"nameIdentifier": "50075", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Hara,  Hiromasa"}], "nameIdentifiers": [{"nameIdentifier": "50076", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Hirabayashi,  Masumi"}], "nameIdentifiers": [{"nameIdentifier": "50077", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Hochi,  Shinichi"}], "nameIdentifiers": [{"nameIdentifier": "50078", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "\u30d5\u30a1\u30a4\u30eb\u60c5\u5831", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2016-03-24"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "High_revivability_of_vitrified-warmed.pdf", "filesize": [{"value": "336.9 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensefree": "This is not the definitive version of record of this article.This manuscript has been accepted for publication in REPRODUCTION, but the version presented here has not yet been copy-edited, formatted or proofed. 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