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pH measurement of tubular vacuoles of an arbuscular mycorrhizal fungus, Gigaspora margarita
http://hdl.handle.net/10091/00019220
http://hdl.handle.net/10091/00019220f43d3f95-03e3-4003-b0de-60198f562167
| 名前 / ファイル | ライセンス | アクション |
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pH_measurement_tubular_vacuoles_arbuscular_mycorrhizal_fungus.pdf (323.9 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||||||||||||
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| 公開日 | 2016-09-29 | |||||||||||||||
| タイトル | ||||||||||||||||
| タイトル | pH measurement of tubular vacuoles of an arbuscular mycorrhizal fungus, Gigaspora margarita | |||||||||||||||
| 言語 | ||||||||||||||||
| 言語 | eng | |||||||||||||||
| DOI | ||||||||||||||||
| 関連識別子 | https://doi.org/10.1007/s00572-014-0588-1 | |||||||||||||||
| 関連名称 | 10.1007/s00572-014-0588-1 | |||||||||||||||
| キーワード | ||||||||||||||||
| 主題 | 6-Carboxyfluorescein diacetate, Arbuscular mycorrhizal symbiosis, Laser scanning confocal microscopy, Tubular vacuole, Vacuolar pH | |||||||||||||||
| 資源タイプ | ||||||||||||||||
| 資源 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||||
| タイプ | journal article | |||||||||||||||
| 著者 |
Funamoto, Rintaro
× Funamoto, Rintaro
× Saito, Katsuharu
× Oyaizu, Hiroshi
× Aono, Toshihiro
× Saito, Masanori
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| 信州大学研究者総覧へのリンク | ||||||||||||||||
| 氏名 | Saito, Katsuharu | |||||||||||||||
| URL | http://soar-rd.shinshu-u.ac.jp/profile/ja.HCkpPUkh.html | |||||||||||||||
| 出版者 | ||||||||||||||||
| 出版者 | SPRINGER | |||||||||||||||
| 引用 | ||||||||||||||||
| 内容記述 | MYCORRHIZA. 25(1):55-60 (2015) | |||||||||||||||
| 書誌情報 |
MYCORRHIZA 巻 25, 号 1, p. 55-60, 発行日 2015-01 |
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| 抄録 | ||||||||||||||||
| 内容記述 | Arbuscular mycorrhizal fungi play an important role in phosphate supply to the host plants. The fungal hyphae contain tubular vacuoles where phosphate compounds such as polyphosphate are accumulated. Despite their importance for the phosphate storage, little is known about the physiological properties of the tubular vacuoles in arbuscular mycorrhizal fungi. As an indicator of the physiological state in vacuoles, we measured pH of tubular vacuoles in living hyphae of arbuscular mycorrhizal fungus Gigaspora margarita using ratio image analysis with pH-dependent fluorescent probe, 6-carboxyfluorescein. Fluorescent images of the fine tubular vacuoles were obtained using a laser scanning confocal microscope, which enabled calculation of vacuolar pH with high spatial resolution. The tubular vacuoles showed mean pH of 5.6 and a pH range of 5.1-6.3. These results suggest that the tubular vacuoles of arbuscular mycorrhizal fungi have a mildly acidic pH just like vacuoles of other fungal species including yeast and ectomycorrhizal fungi. | |||||||||||||||
| 資源タイプ（コンテンツの種類） | ||||||||||||||||
| ISSN | ||||||||||||||||
| 収録物識別子タイプ | ISSN | |||||||||||||||
| 収録物識別子 | 0940-6360 | |||||||||||||||
| 書誌レコードID | ||||||||||||||||
| 収録物識別子タイプ | NCID | |||||||||||||||
| 収録物識別子 | AA10825451 | |||||||||||||||
| PubMed | ||||||||||||||||
| 識別子タイプ | PMID | |||||||||||||||
| 関連識別子 | https://pubmed.ncbi.nlm.nih.gov/24838377/ | |||||||||||||||
| 関連名称 | 24838377 | |||||||||||||||
| 権利 | ||||||||||||||||
| 権利情報 | The original publication is available at www.springerlink.com | |||||||||||||||
| 出版タイプ | ||||||||||||||||
| 出版タイプ | AM | |||||||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||||||||||||
| WoS | ||||||||||||||||
| URL | http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=ShinshuUniv&SrcApp=ShinshuUniv&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000347158800005 | |||||||||||||||
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Funamoto, Rintaro, Saito, Katsuharu, Oyaizu, Hiroshi, Aono, Toshihiro, Saito, Masanori, 2015, pH measurement of tubular vacuoles of an arbuscular mycorrhizal fungus, Gigaspora margarita: SPRINGER, 55–60 p.
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