{"_buckets": {"deposit": "5331d777-abfd-4eeb-8410-a372564cf747"}, "_deposit": {"id": "18458", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "18458"}, "status": "published"}, "_oai": {"id": "oai:soar-ir.repo.nii.ac.jp:00018458", "sets": ["1016:1017"]}, "author_link": ["51381", "51382", "51383", "51384", "51385"], "item_1628147817048": {"attribute_name": "\u51fa\u7248\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_ab4af688f83e57aa", "subitem_version_type": "AM"}]}, "item_6_biblio_info_6": {"attribute_name": "\u66f8\u8a8c\u60c5\u5831", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2015-01", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "1", "bibliographicPageEnd": "60", "bibliographicPageStart": "55", "bibliographicVolumeNumber": "25", "bibliographic_titles": [{"bibliographic_title": "MYCORRHIZA"}]}]}, "item_6_description_20": {"attribute_name": "\u6284\u9332", "attribute_value_mlt": [{"subitem_description": "Arbuscular mycorrhizal fungi play an important role in phosphate supply to the host plants. The fungal hyphae contain tubular vacuoles where phosphate compounds such as polyphosphate are accumulated. Despite their importance for the phosphate storage, little is known about the physiological properties of the tubular vacuoles in arbuscular mycorrhizal fungi. As an indicator of the physiological state in vacuoles, we measured pH of tubular vacuoles in living hyphae of arbuscular mycorrhizal fungus Gigaspora margarita using ratio image analysis with pH-dependent fluorescent probe, 6-carboxyfluorescein. Fluorescent images of the fine tubular vacuoles were obtained using a laser scanning confocal microscope, which enabled calculation of vacuolar pH with high spatial resolution. The tubular vacuoles showed mean pH of 5.6 and a pH range of 5.1-6.3. These results suggest that the tubular vacuoles of arbuscular mycorrhizal fungi have a mildly acidic pH just like vacuoles of other fungal species including yeast and ectomycorrhizal fungi.", "subitem_description_type": "Abstract"}]}, "item_6_description_30": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7\uff08\u30b3\u30f3\u30c6\u30f3\u30c4\u306e\u7a2e\u985e\uff09", "attribute_value_mlt": [{"subitem_description": "Article", "subitem_description_type": "Other"}]}, "item_6_description_5": {"attribute_name": "\u5f15\u7528", "attribute_value_mlt": [{"subitem_description": "MYCORRHIZA. 25(1):55-60 (2015)", "subitem_description_type": "Other"}]}, "item_6_link_3": {"attribute_name": "\u4fe1\u5dde\u5927\u5b66\u7814\u7a76\u8005\u7dcf\u89a7\u3078\u306e\u30ea\u30f3\u30af", "attribute_value_mlt": [{"subitem_link_text": "Saito, Katsuharu", "subitem_link_url": "http://soar-rd.shinshu-u.ac.jp/profile/ja.HCkpPUkh.html"}]}, "item_6_link_67": {"attribute_name": "WoS", "attribute_value_mlt": [{"subitem_link_text": "Web of Science", "subitem_link_url": "http://gateway.isiknowledge.com/gateway/Gateway.cgi?\u0026GWVersion=2\u0026SrcAuth=ShinshuUniv\u0026SrcApp=ShinshuUniv\u0026DestLinkType=FullRecord\u0026DestApp=WOS\u0026KeyUT=000347158800005"}]}, "item_6_publisher_4": {"attribute_name": "\u51fa\u7248\u8005", "attribute_value_mlt": [{"subitem_publisher": "SPRINGER"}]}, "item_6_relation_47": {"attribute_name": "PubMed", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "24838377"}], "subitem_relation_type_id": {"subitem_relation_type_id_text": "https://pubmed.ncbi.nlm.nih.gov/24838377/", "subitem_relation_type_select": "PMID"}}]}, "item_6_relation_48": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "10.1007/s00572-014-0588-1"}], "subitem_relation_type_id": {"subitem_relation_type_id_text": "https://doi.org/10.1007/s00572-014-0588-1", "subitem_relation_type_select": "DOI"}}]}, "item_6_rights_62": {"attribute_name": "\u6a29\u5229", "attribute_value_mlt": [{"subitem_rights": "The original publication is available at www.springerlink.com"}]}, "item_6_select_64": {"attribute_name": "\u8457\u8005\u7248\u30d5\u30e9\u30b0", "attribute_value_mlt": [{"subitem_select_item": "author"}]}, "item_6_source_id_35": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "0940-6360", "subitem_source_identifier_type": "ISSN"}]}, "item_6_source_id_39": {"attribute_name": "NII ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "0940-6360", "subitem_source_identifier_type": "ISSN"}]}, "item_6_source_id_40": {"attribute_name": "\u66f8\u8a8c\u30ec\u30b3\u30fc\u30c9ID", "attribute_value_mlt": [{"subitem_source_identifier": "AA10825451", "subitem_source_identifier_type": "NCID"}]}, "item_6_text_69": {"attribute_name": "wosonly authkey", "attribute_value_mlt": [{"subitem_text_value": "6-Carboxyfluorescein diacetate; Arbuscular mycorrhizal symbiosis; Laser scanning confocal microscopy; Tubular vacuole; Vacuolar pH"}]}, "item_6_text_70": {"attribute_name": "wosonly keywords", "attribute_value_mlt": [{"subitem_text_value": "SACCHAROMYCES-CEREVISIAE; PISOLITHUS-TINCTORIUS; INTRACELLULAR PH; GROWING HYPHAE; P-31 NMR; POLYPHOSPHATE; TRANSPORT; SYSTEM; TRANSLOCATION; METABOLISM"}]}, "item_6_textarea_68": {"attribute_name": "wosonly abstract", "attribute_value_mlt": [{"subitem_textarea_value": "Arbuscular mycorrhizal fungi play an important role in phosphate supply to the host plants. The fungal hyphae contain tubular vacuoles where phosphate compounds such as polyphosphate are accumulated. Despite their importance for the phosphate storage, little is known about the physiological properties of the tubular vacuoles in arbuscular mycorrhizal fungi. As an indicator of the physiological state in vacuoles, we measured pH of tubular vacuoles in living hyphae of arbuscular mycorrhizal fungus Gigaspora margarita using ratio image analysis with pH-dependent fluorescent probe, 6-carboxyfluorescein. Fluorescent images of the fine tubular vacuoles were obtained using a laser scanning confocal microscope, which enabled calculation of vacuolar pH with high spatial resolution. The tubular vacuoles showed mean pH of 5.6 and a pH range of 5.1-6.3. These results suggest that the tubular vacuoles of arbuscular mycorrhizal fungi have a mildly acidic pH just like vacuoles of other fungal species including yeast and ectomycorrhizal fungi."}]}, "item_creator": {"attribute_name": "\u8457\u8005", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Funamoto,  Rintaro"}], "nameIdentifiers": [{"nameIdentifier": "51381", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Saito,  Katsuharu"}], "nameIdentifiers": [{"nameIdentifier": "51382", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Oyaizu,  Hiroshi"}], "nameIdentifiers": [{"nameIdentifier": "51383", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Aono,  Toshihiro"}], "nameIdentifiers": [{"nameIdentifier": "51384", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Saito,  Masanori"}], "nameIdentifiers": [{"nameIdentifier": "51385", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "\u30d5\u30a1\u30a4\u30eb\u60c5\u5831", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2016-09-29"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "pH_measurement_tubular_vacuoles_arbuscular_mycorrhizal_fungus.pdf", "filesize": [{"value": "323.9 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensefree": "The original publication is available at www.springerlink.com", "licensetype": "license_note", "mimetype": "application/pdf", "size": 323900.0, "url": {"label": "pH_measurement_tubular_vacuoles_arbuscular_mycorrhizal_fungus.pdf", "url": "https://soar-ir.repo.nii.ac.jp/record/18458/files/pH_measurement_tubular_vacuoles_arbuscular_mycorrhizal_fungus.pdf"}, "version_id": "be40f597-cd6e-4f15-89bc-1e78ec56e8c4"}]}, "item_keyword": {"attribute_name": "\u30ad\u30fc\u30ef\u30fc\u30c9", "attribute_value_mlt": [{"subitem_subject": "6-Carboxyfluorescein diacetate", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Arbuscular mycorrhizal symbiosis", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Laser scanning confocal microscopy", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Tubular vacuole", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Vacuolar pH", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "\u8a00\u8a9e", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "pH measurement of tubular vacuoles of an arbuscular mycorrhizal fungus, Gigaspora margarita", "item_titles": {"attribute_name": "\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_title": "pH measurement of tubular vacuoles of an arbuscular mycorrhizal fungus, Gigaspora margarita", "subitem_title_language": "en"}]}, "item_type_id": "6", "owner": "1", "path": ["1016/1017"], "permalink_uri": "http://hdl.handle.net/10091/00019220", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2016-09-29"}, "publish_date": "2016-09-29", "publish_status": "0", "recid": "18458", "relation": {}, "relation_version_is_last": true, "title": ["pH measurement of tubular vacuoles of an arbuscular mycorrhizal fungus, Gigaspora margarita"], "weko_shared_id": -1}