Item type |
学術雑誌論文 / Journal Article(1) |
公開日 |
2016-10-28 |
タイトル |
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タイトル |
Heterologous Expression and Functional Characterization of a Novel Chitinase from the Chitinolytic Bacterium Chitiniphilus shinanonensis |
言語 |
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言語 |
eng |
DOI |
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関連識別子 |
https://doi.org/10.1271/bbb.110822 |
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関連名称 |
10.1271/bbb.110822 |
キーワード |
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主題 |
Chitiniphilus shinanonensis, chitinase, chitin-binding domain, N-acetyl-D-glucosamine |
資源タイプ |
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資源 |
http://purl.org/coar/resource_type/c_6501 |
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タイプ |
journal article |
著者 |
Huang, Lanxiang
Shizume, Arisa
Nogawa, Masahiro
Taguchi, Goro
Shimosaka, Makoto
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信州大学研究者総覧へのリンク |
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氏名 |
Nogawa, Masahiro |
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URL |
http://soar-rd.shinshu-u.ac.jp/profile/ja.gNcajNnU.html |
信州大学研究者総覧へのリンク |
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氏名 |
Taguchi, Goro |
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URL |
http://soar-rd.shinshu-u.ac.jp/profile/ja.uCDpjekV.html |
信州大学研究者総覧へのリンク |
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氏名 |
Shimosaka, Makoto |
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URL |
http://soar-rd.shinshu-u.ac.jp/profile/ja.ZekmjekV.html |
出版者 |
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出版者 |
TAYLOR & FRANCIS LTD |
引用 |
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内容記述 |
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY. 76(3):517-522 (2012) |
書誌情報 |
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
巻 76,
号 3,
p. 517-522,
発行日 2012-03
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抄録 |
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内容記述 |
Chitiniphilus shinanonensis strain SAY3(T) is a chitinolytic bacterium isolated from moat water of Ueda Castle in Nagano Prefecture, Japan. Fifteen genes encoding putative chitinolytic enzymes (chiA-chiO) have been isolated from this bacterium. Five of these constitute a single operon (chiCDEFG). The open reading frames of chiC, chiD, chiE, and chiG show sequence similarity to family 18 chitinases, while chiF encodes a polypeptide with two chitin-binding domains but no catalytic domain. Each of the five genes was successfully expressed in Escherichia coli, and the resulting recombinant proteins were characterized. Four of the recombinant proteins (ChiC, ChiD, ChiE, and ChiG) exhibited endo-type chitinase activity toward chitinous substrates, while ChiF showed no chitinolytic activity. In contrast to most endo-type chitinases, which mainly produce a dimer of N-acetyl-D-glucosamine (GlcNAc) as final product, ChiG completely split the GlcNAc dimer into GlcNAc monomers, indicating that it is a novel chitinase. |
資源タイプ(コンテンツの種類) |
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内容記述 |
Article |
ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
0916-8451 |
書誌レコードID |
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収録物識別子タイプ |
NCID |
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収録物識別子 |
AA10824164 |
PubMed |
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関連識別子 |
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?CMD=search&DB=pubmed&term=22451394 |
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関連名称 |
22451394 |
権利 |
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権利情報 |
This is an Accepted Manuscript of an article published by Taylor & Francis in Bioscience, Biotechnology, and Biochemistry on 2014, available online: http://www.tandfonline.com/ , https://doi.org/10.1271/bbb.110822 . |
出版タイプ |
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出版タイプ |
AM |
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出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
WoS |
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URL |
http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=ShinshuUniv&SrcApp=ShinshuUniv&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000302667600014 |