{"_buckets": {"deposit": "c65f033a-b4ef-4cf3-b574-2ec1a3623102"}, "_deposit": {"id": "18586", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "18586"}, "status": "published"}, "_oai": {"id": "oai:soar-ir.repo.nii.ac.jp:00018586"}, "item_6_biblio_info_6": {"attribute_name": "\u66f8\u8a8c\u60c5\u5831", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2017-01", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "1", "bibliographicPageEnd": "349", "bibliographicPageStart": "341", "bibliographicVolumeNumber": "101", "bibliographic_titles": [{"bibliographic_title": "APPLIED MICROBIOLOGY AND BIOTECHNOLOGY"}]}]}, "item_6_description_19": {"attribute_name": "\u5185\u5bb9\u8a18\u8ff0", "attribute_value_mlt": [{"subitem_description": "Epub 2016 Oct 8.", "subitem_description_type": "Other"}]}, "item_6_description_20": {"attribute_name": "\u6284\u9332", "attribute_value_mlt": [{"subitem_description": "Interleukin 6 (IL-6) is an important pathogenic factor in development of various inflammatory and autoimmune diseases and cancer. Blocking antibodies against molecules associated with IL-6/IL-6 receptor signaling are an attractive candidate for the prevention or therapy of these diseases. In this study, we developed a genetically modified strain of Lactococcus lactis secreting a single-chain variable fragment antibody against mouse IL-6 (IL6scFv). An IL6scFv-secretion vector was constructed by cloning an IL6scFv gene fragment into a lactococcal secretion plasmid and was electroporated into L. lactis NZ9000 (NZ-IL6scFv). Secretion of recombinant IL6scFv (rIL6scFv) by nisin-induced NZ-IL6scFv was confirmed by western blotting and was optimized by tuning culture conditions. We found that rIL6scFv could bind to commercial recombinant mouse IL-6. This result clearly demonstrated the immunoreactivity of rIL6scFv. This is the first study to engineer a genetically modified strain of lactic acid bacteria (gmLAB) that produces a functional anti-cytokine scFv. Numerous previous studies suggested that mucosal delivery of biomedical proteins using gmLAB is an effective and low-cost way to treat various disorders. Therefore, NZ-IL6scFv may be an attractive tool for the research and development of new IL-6 targeting agents for various inflammatory and autoimmune diseases as well as for cancer.", "subitem_description_type": "Abstract"}]}, "item_6_description_30": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7\uff08\u30b3\u30f3\u30c6\u30f3\u30c4\u306e\u7a2e\u985e\uff09", "attribute_value_mlt": [{"subitem_description": "Article", "subitem_description_type": "Other"}]}, "item_6_description_5": {"attribute_name": "\u5f15\u7528", "attribute_value_mlt": [{"subitem_description": "APPLIED MICROBIOLOGY AND BIOTECHNOLOGY. 101(1):341-349 (2017)", "subitem_description_type": "Other"}]}, "item_6_link_3": {"attribute_name": "\u4fe1\u5dde\u5927\u5b66\u7814\u7a76\u8005\u7dcf\u89a7\u3078\u306e\u30ea\u30f3\u30af", "attribute_value_mlt": [{"subitem_link_text": "Ihara, Masaki", "subitem_link_url": "http://soar-rd.shinshu-u.ac.jp/profile/ja.jpkaWUyV.html"}, {"subitem_link_text": "Ogita, Tasuku", "subitem_link_url": "http://soar-rd.shinshu-u.ac.jp/profile/ja.yhcePhkh.html"}, {"subitem_link_text": "Shimosato, Takeshi", "subitem_link_url": "http://soar-rd.shinshu-u.ac.jp/profile/ja.ONLCjFkV.html"}]}, "item_6_publisher_4": {"attribute_name": "\u51fa\u7248\u8005", "attribute_value_mlt": [{"subitem_publisher": "SPRINGER"}]}, "item_6_relation_47": {"attribute_name": "PubMed", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "27722916"}], "subitem_relation_type_id": {"subitem_relation_type_id_text": "http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?CMD=search\u0026DB=pubmed\u0026term=27722916", "subitem_relation_type_select": "PMID"}}]}, "item_6_relation_48": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "10.1007/s00253-016-7907-8"}], "subitem_relation_type_id": {"subitem_relation_type_id_text": "http://dx.doi.org/10.1007/s00253-016-7907-8", "subitem_relation_type_select": "DOI"}}]}, 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"attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2017-10-08"}], "displaytype": "detail", "download_preview_message": "", "filename": "Secretion_immunoreactive_single-chain_variable_fragment.pdf", "filesize": [{"value": "4.8 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensefree": "The original publication is available at www.springerlink.com", "licensetype": "license_free", "mimetype": "application/pdf", "size": 48000000, "url": {"label": "Secretion_immunoreactive_single-chain_variable_fragment.pdf", "url": "https://soar-ir.repo.nii.ac.jp/record/18586/files/Secretion_immunoreactive_single-chain_variable_fragment.pdf"}, "version_id": "bf94d0b3-17e1-458d-b76a-4462eefd5bda"}]}, "item_keyword": {"attribute_name": "\u30ad\u30fc\u30ef\u30fc\u30c9", "attribute_value_mlt": [{"subitem_subject": "Immunoreactivity", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Interleukin 6", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Lactococcus lactis", "subitem_subject_scheme": "Other"}, {"subitem_subject": "gmLAB", "subitem_subject_scheme": "Other"}, {"subitem_subject": "scFv antibody", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "\u8a00\u8a9e", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Secretion of an immunoreactive single-chain variable fragment antibody against mouse interleukin 6 by Lactococcus lactis", "item_titles": {"attribute_name": "\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_title": "Secretion of an immunoreactive single-chain variable fragment antibody against mouse interleukin 6 by Lactococcus lactis"}]}, "item_type_id": "6", "owner": "1", "path": ["1016/1017"], "permalink_uri": "http://hdl.handle.net/10091/00019348", "pubdate": {"attribute_name": "\u516c\u958b\u65e5", "attribute_name_i18n": "\u516c\u958b\u65e5", "attribute_value": "2017-02-01"}, "publish_date": "2017-02-01", "publish_status": "0", "recid": "18586", "relation": {}, "relation_version_is_last": true, "title": ["Secretion of an immunoreactive single-chain variable fragment antibody against mouse interleukin 6 by Lactococcus lactis"], "weko_shared_id": null}