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Secretion of an immunoreactive single-chain variable fragment antibody against mouse interleukin 6 by Lactococcus lactis
http://hdl.handle.net/10091/00019348
http://hdl.handle.net/10091/00019348272432c7-24ed-434e-9571-b698edbb3fd5
| 名前 / ファイル | ライセンス | アクション |
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Secretion_immunoreactive_single-chain_variable_fragment.pdf (4.8 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||||||||||||||||
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| 公開日 | 2017-02-01 | |||||||||||||||||||
| タイトル | ||||||||||||||||||||
| タイトル | Secretion of an immunoreactive single-chain variable fragment antibody against mouse interleukin 6 by Lactococcus lactis | |||||||||||||||||||
| 言語 | ||||||||||||||||||||
| 言語 | eng | |||||||||||||||||||
| DOI | ||||||||||||||||||||
| 関連識別子 | https://doi.org/10.1007/s00253-016-7907-8 | |||||||||||||||||||
| 関連名称 | 10.1007/s00253-016-7907-8 | |||||||||||||||||||
| キーワード | ||||||||||||||||||||
| 主題 | Immunoreactivity, Interleukin 6, Lactococcus lactis, gmLAB, scFv antibody | |||||||||||||||||||
| 資源タイプ | ||||||||||||||||||||
| 資源 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||||||||
| タイプ | journal article | |||||||||||||||||||
| 著者 |
Shigemori, Suguru
× Shigemori, Suguru
× Ihara, Masaki
× Sato, Takashi
× Yamamoto, Yoshinari
× Nigar, Shireen
× Ogita, Tasuku
× Shimosato, Takeshi
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| 信州大学研究者総覧へのリンク | ||||||||||||||||||||
| 氏名 | Ihara, Masaki | |||||||||||||||||||
| URL | http://soar-rd.shinshu-u.ac.jp/profile/ja.jpkaWUyV.html | |||||||||||||||||||
| 信州大学研究者総覧へのリンク | ||||||||||||||||||||
| 氏名 | Ogita, Tasuku | |||||||||||||||||||
| URL | http://soar-rd.shinshu-u.ac.jp/profile/ja.yhcePhkh.html | |||||||||||||||||||
| 信州大学研究者総覧へのリンク | ||||||||||||||||||||
| 氏名 | Shimosato, Takeshi | |||||||||||||||||||
| URL | http://soar-rd.shinshu-u.ac.jp/profile/ja.ONLCjFkV.html | |||||||||||||||||||
| 出版者 | ||||||||||||||||||||
| 出版者 | SPRINGER | |||||||||||||||||||
| 引用 | ||||||||||||||||||||
| 内容記述 | APPLIED MICROBIOLOGY AND BIOTECHNOLOGY. 101(1):341-349 (2017) | |||||||||||||||||||
| 書誌情報 |
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 巻 101, 号 1, p. 341-349, 発行日 2017-01 |
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| 内容記述 | ||||||||||||||||||||
| 内容記述タイプ | Other | |||||||||||||||||||
| 内容記述 | Epub 2016 Oct 8. | |||||||||||||||||||
| 抄録 | ||||||||||||||||||||
| 内容記述 | Interleukin 6 (IL-6) is an important pathogenic factor in development of various inflammatory and autoimmune diseases and cancer. Blocking antibodies against molecules associated with IL-6/IL-6 receptor signaling are an attractive candidate for the prevention or therapy of these diseases. In this study, we developed a genetically modified strain of Lactococcus lactis secreting a single-chain variable fragment antibody against mouse IL-6 (IL6scFv). An IL6scFv-secretion vector was constructed by cloning an IL6scFv gene fragment into a lactococcal secretion plasmid and was electroporated into L. lactis NZ9000 (NZ-IL6scFv). Secretion of recombinant IL6scFv (rIL6scFv) by nisin-induced NZ-IL6scFv was confirmed by western blotting and was optimized by tuning culture conditions. We found that rIL6scFv could bind to commercial recombinant mouse IL-6. This result clearly demonstrated the immunoreactivity of rIL6scFv. This is the first study to engineer a genetically modified strain of lactic acid bacteria (gmLAB) that produces a functional anti-cytokine scFv. Numerous previous studies suggested that mucosal delivery of biomedical proteins using gmLAB is an effective and low-cost way to treat various disorders. Therefore, NZ-IL6scFv may be an attractive tool for the research and development of new IL-6 targeting agents for various inflammatory and autoimmune diseases as well as for cancer. | |||||||||||||||||||
| 資源タイプ（コンテンツの種類） | ||||||||||||||||||||
| ISSN | ||||||||||||||||||||
| 収録物識別子タイプ | ISSN | |||||||||||||||||||
| 収録物識別子 | 0175-7598 | |||||||||||||||||||
| 書誌レコードID | ||||||||||||||||||||
| 収録物識別子タイプ | NCID | |||||||||||||||||||
| 収録物識別子 | AA10503386 | |||||||||||||||||||
| PubMed | ||||||||||||||||||||
| 識別子タイプ | PMID | |||||||||||||||||||
| 関連識別子 | https://pubmed.ncbi.nlm.nih.gov/27722916/ | |||||||||||||||||||
| 関連名称 | 27722916 | |||||||||||||||||||
| 権利 | ||||||||||||||||||||
| 権利情報 | The original publication is available at www.springerlink.com | |||||||||||||||||||
| 出版タイプ | ||||||||||||||||||||
| 出版タイプ | AM | |||||||||||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||||||||||||||||
