Item type |
学術雑誌論文 / Journal Article(1) |
公開日 |
2017-02-01 |
タイトル |
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タイトル |
Secretion of an immunoreactive single-chain variable fragment antibody against mouse interleukin 6 by Lactococcus lactis |
言語 |
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言語 |
eng |
DOI |
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関連識別子 |
https://doi.org/10.1007/s00253-016-7907-8 |
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関連名称 |
10.1007/s00253-016-7907-8 |
キーワード |
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主題 |
Immunoreactivity, Interleukin 6, Lactococcus lactis, gmLAB, scFv antibody |
資源タイプ |
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資源 |
http://purl.org/coar/resource_type/c_6501 |
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タイプ |
journal article |
著者 |
Shigemori, Suguru
Ihara, Masaki
Sato, Takashi
Yamamoto, Yoshinari
Nigar, Shireen
Ogita, Tasuku
Shimosato, Takeshi
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信州大学研究者総覧へのリンク |
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氏名 |
Ihara, Masaki |
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URL |
http://soar-rd.shinshu-u.ac.jp/profile/ja.jpkaWUyV.html |
信州大学研究者総覧へのリンク |
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氏名 |
Ogita, Tasuku |
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URL |
http://soar-rd.shinshu-u.ac.jp/profile/ja.yhcePhkh.html |
信州大学研究者総覧へのリンク |
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氏名 |
Shimosato, Takeshi |
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URL |
http://soar-rd.shinshu-u.ac.jp/profile/ja.ONLCjFkV.html |
出版者 |
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出版者 |
SPRINGER |
引用 |
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内容記述 |
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY. 101(1):341-349 (2017) |
書誌情報 |
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
巻 101,
号 1,
p. 341-349,
発行日 2017-01
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内容記述 |
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内容記述 |
Epub 2016 Oct 8. |
抄録 |
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内容記述 |
Interleukin 6 (IL-6) is an important pathogenic factor in development of various inflammatory and autoimmune diseases and cancer. Blocking antibodies against molecules associated with IL-6/IL-6 receptor signaling are an attractive candidate for the prevention or therapy of these diseases. In this study, we developed a genetically modified strain of Lactococcus lactis secreting a single-chain variable fragment antibody against mouse IL-6 (IL6scFv). An IL6scFv-secretion vector was constructed by cloning an IL6scFv gene fragment into a lactococcal secretion plasmid and was electroporated into L. lactis NZ9000 (NZ-IL6scFv). Secretion of recombinant IL6scFv (rIL6scFv) by nisin-induced NZ-IL6scFv was confirmed by western blotting and was optimized by tuning culture conditions. We found that rIL6scFv could bind to commercial recombinant mouse IL-6. This result clearly demonstrated the immunoreactivity of rIL6scFv. This is the first study to engineer a genetically modified strain of lactic acid bacteria (gmLAB) that produces a functional anti-cytokine scFv. Numerous previous studies suggested that mucosal delivery of biomedical proteins using gmLAB is an effective and low-cost way to treat various disorders. Therefore, NZ-IL6scFv may be an attractive tool for the research and development of new IL-6 targeting agents for various inflammatory and autoimmune diseases as well as for cancer. |
資源タイプ(コンテンツの種類) |
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内容記述 |
Article |
ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
0175-7598 |
書誌レコードID |
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収録物識別子タイプ |
NCID |
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収録物識別子 |
AA10503386 |
PubMed |
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関連識別子 |
https://pubmed.ncbi.nlm.nih.gov/27722916/ |
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関連名称 |
27722916 |
権利 |
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権利情報 |
The original publication is available at www.springerlink.com |
出版タイプ |
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出版タイプ |
AM |
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出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |