{"_buckets": {"deposit": "c65f033a-b4ef-4cf3-b574-2ec1a3623102"}, "_deposit": {"id": "18586", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "18586"}, "status": "published"}, "_oai": {"id": "oai:soar-ir.repo.nii.ac.jp:00018586", "sets": ["1016:1017"]}, "author_link": ["102847", "102848", "102849", "102850", "102851", "102852", "102853"], "item_1628147817048": {"attribute_name": "\u51fa\u7248\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_ab4af688f83e57aa", "subitem_version_type": "AM"}]}, "item_6_biblio_info_6": {"attribute_name": "\u66f8\u8a8c\u60c5\u5831", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2017-01", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "1", "bibliographicPageEnd": "349", "bibliographicPageStart": "341", "bibliographicVolumeNumber": "101", "bibliographic_titles": [{"bibliographic_title": "APPLIED MICROBIOLOGY AND BIOTECHNOLOGY"}]}]}, "item_6_description_19": {"attribute_name": "\u5185\u5bb9\u8a18\u8ff0", "attribute_value_mlt": [{"subitem_description": "Epub 2016 Oct 8.", "subitem_description_type": "Other"}]}, "item_6_description_20": {"attribute_name": "\u6284\u9332", "attribute_value_mlt": [{"subitem_description": "Interleukin 6 (IL-6) is an important pathogenic factor in development of various inflammatory and autoimmune diseases and cancer. Blocking antibodies against molecules associated with IL-6/IL-6 receptor signaling are an attractive candidate for the prevention or therapy of these diseases. In this study, we developed a genetically modified strain of Lactococcus lactis secreting a single-chain variable fragment antibody against mouse IL-6 (IL6scFv). An IL6scFv-secretion vector was constructed by cloning an IL6scFv gene fragment into a lactococcal secretion plasmid and was electroporated into L. lactis NZ9000 (NZ-IL6scFv). Secretion of recombinant IL6scFv (rIL6scFv) by nisin-induced NZ-IL6scFv was confirmed by western blotting and was optimized by tuning culture conditions. We found that rIL6scFv could bind to commercial recombinant mouse IL-6. This result clearly demonstrated the immunoreactivity of rIL6scFv. This is the first study to engineer a genetically modified strain of lactic acid bacteria (gmLAB) that produces a functional anti-cytokine scFv. Numerous previous studies suggested that mucosal delivery of biomedical proteins using gmLAB is an effective and low-cost way to treat various disorders. Therefore, NZ-IL6scFv may be an attractive tool for the research and development of new IL-6 targeting agents for various inflammatory and autoimmune diseases as well as for cancer.", "subitem_description_type": "Abstract"}]}, "item_6_description_30": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7\uff08\u30b3\u30f3\u30c6\u30f3\u30c4\u306e\u7a2e\u985e\uff09", "attribute_value_mlt": [{"subitem_description": "Article", "subitem_description_type": "Other"}]}, "item_6_description_5": {"attribute_name": "\u5f15\u7528", "attribute_value_mlt": [{"subitem_description": "APPLIED MICROBIOLOGY AND BIOTECHNOLOGY. 101(1):341-349 (2017)", "subitem_description_type": "Other"}]}, "item_6_link_3": {"attribute_name": "\u4fe1\u5dde\u5927\u5b66\u7814\u7a76\u8005\u7dcf\u89a7\u3078\u306e\u30ea\u30f3\u30af", "attribute_value_mlt": [{"subitem_link_text": "Ihara, Masaki", "subitem_link_url": "http://soar-rd.shinshu-u.ac.jp/profile/ja.jpkaWUyV.html"}, {"subitem_link_text": "Ogita, Tasuku", "subitem_link_url": "http://soar-rd.shinshu-u.ac.jp/profile/ja.yhcePhkh.html"}, {"subitem_link_text": "Shimosato, Takeshi", "subitem_link_url": "http://soar-rd.shinshu-u.ac.jp/profile/ja.ONLCjFkV.html"}]}, "item_6_publisher_4": {"attribute_name": "\u51fa\u7248\u8005", "attribute_value_mlt": [{"subitem_publisher": "SPRINGER"}]}, "item_6_relation_47": {"attribute_name": "PubMed", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "27722916"}], "subitem_relation_type_id": {"subitem_relation_type_id_text": "https://pubmed.ncbi.nlm.nih.gov/27722916/", "subitem_relation_type_select": "PMID"}}]}, "item_6_relation_48": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "10.1007/s00253-016-7907-8"}], "subitem_relation_type_id": {"subitem_relation_type_id_text": "https://doi.org/10.1007/s00253-016-7907-8", "subitem_relation_type_select": "DOI"}}]}, "item_6_rights_62": {"attribute_name": "\u6a29\u5229", "attribute_value_mlt": [{"subitem_rights": "The original publication is available at www.springerlink.com"}]}, "item_6_select_64": {"attribute_name": "\u8457\u8005\u7248\u30d5\u30e9\u30b0", "attribute_value_mlt": [{"subitem_select_item": "author"}]}, "item_6_source_id_35": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "0175-7598", "subitem_source_identifier_type": "ISSN"}]}, "item_6_source_id_39": {"attribute_name": "NII ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "0175-7598", "subitem_source_identifier_type": "ISSN"}]}, "item_6_source_id_40": {"attribute_name": "\u66f8\u8a8c\u30ec\u30b3\u30fc\u30c9ID", "attribute_value_mlt": [{"subitem_source_identifier": "AA10503386", "subitem_source_identifier_type": "NCID"}]}, "item_creator": {"attribute_name": "\u8457\u8005", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Shigemori,  Suguru"}], "nameIdentifiers": [{"nameIdentifier": "102847", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Ihara,  Masaki"}], "nameIdentifiers": [{"nameIdentifier": "102848", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Sato,  Takashi"}], "nameIdentifiers": [{"nameIdentifier": "102849", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Yamamoto,  Yoshinari"}], "nameIdentifiers": [{"nameIdentifier": "102850", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Nigar,  Shireen"}], "nameIdentifiers": [{"nameIdentifier": "102851", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Ogita,  Tasuku"}], "nameIdentifiers": [{"nameIdentifier": "102852", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Shimosato,  Takeshi"}], "nameIdentifiers": [{"nameIdentifier": "102853", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "\u30d5\u30a1\u30a4\u30eb\u60c5\u5831", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2017-10-08"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "Secretion_immunoreactive_single-chain_variable_fragment.pdf", "filesize": [{"value": "4.8 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensefree": "The original publication is available at www.springerlink.com", "licensetype": "license_note", "mimetype": "application/pdf", "size": 4800000.0, "url": {"label": "Secretion_immunoreactive_single-chain_variable_fragment.pdf", "url": "https://soar-ir.repo.nii.ac.jp/record/18586/files/Secretion_immunoreactive_single-chain_variable_fragment.pdf"}, "version_id": "bf94d0b3-17e1-458d-b76a-4462eefd5bda"}]}, "item_keyword": {"attribute_name": "\u30ad\u30fc\u30ef\u30fc\u30c9", "attribute_value_mlt": [{"subitem_subject": "Immunoreactivity", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Interleukin 6", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Lactococcus lactis", "subitem_subject_scheme": "Other"}, {"subitem_subject": "gmLAB", "subitem_subject_scheme": "Other"}, {"subitem_subject": "scFv antibody", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "\u8a00\u8a9e", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Secretion of an immunoreactive single-chain variable fragment antibody against mouse interleukin 6 by Lactococcus lactis", "item_titles": {"attribute_name": "\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_title": "Secretion of an immunoreactive single-chain variable fragment antibody against mouse interleukin 6 by Lactococcus lactis", "subitem_title_language": "en"}]}, "item_type_id": "6", "owner": "1", "path": ["1016/1017"], "permalink_uri": "http://hdl.handle.net/10091/00019348", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2017-02-01"}, "publish_date": "2017-02-01", "publish_status": "0", "recid": "18586", "relation": {}, "relation_version_is_last": true, "title": ["Secretion of an immunoreactive single-chain variable fragment antibody against mouse interleukin 6 by Lactococcus lactis"], "weko_shared_id": -1}