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  1. 070 農学部, 大学院農学研究科
  2. 0701 学術論文

Secretion of an immunoreactive single-chain variable fragment antibody against mouse interleukin 6 by Lactococcus lactis

http://hdl.handle.net/10091/00019348
272432c7-24ed-434e-9571-b698edbb3fd5
名前 / ファイル ライセンス アクション
Secretion_immunoreactive_single-chain_variable_fragment.pdf Secretion_immunoreactive_single-chain_variable_fragment.pdf (4.8 MB)
Item type 学術雑誌論文 / Journal Article(1)
公開日 2017-02-01
タイトル
言語 en
タイトル Secretion of an immunoreactive single-chain variable fragment antibody against mouse interleukin 6 by Lactococcus lactis
言語
言語 eng
キーワード
主題Scheme Other
主題 Immunoreactivity
キーワード
主題Scheme Other
主題 Interleukin 6
キーワード
主題Scheme Other
主題 Lactococcus lactis
キーワード
主題Scheme Other
主題 gmLAB
キーワード
主題Scheme Other
主題 scFv antibody
資源タイプ
資源 http://purl.org/coar/resource_type/c_6501
タイプ journal article
著者 Shigemori, Suguru

× Shigemori, Suguru

WEKO 102847

Shigemori, Suguru

Search repository
Ihara, Masaki

× Ihara, Masaki

WEKO 102848

Ihara, Masaki

Search repository
Sato, Takashi

× Sato, Takashi

WEKO 102849

Sato, Takashi

Search repository
Yamamoto, Yoshinari

× Yamamoto, Yoshinari

WEKO 102850

Yamamoto, Yoshinari

Search repository
Nigar, Shireen

× Nigar, Shireen

WEKO 102851

Nigar, Shireen

Search repository
Ogita, Tasuku

× Ogita, Tasuku

WEKO 102852

Ogita, Tasuku

Search repository
Shimosato, Takeshi

× Shimosato, Takeshi

WEKO 102853

Shimosato, Takeshi

Search repository
信州大学研究者総覧へのリンク
氏名 Ihara, Masaki
URL http://soar-rd.shinshu-u.ac.jp/profile/ja.jpkaWUyV.html
信州大学研究者総覧へのリンク
氏名 Ogita, Tasuku
URL http://soar-rd.shinshu-u.ac.jp/profile/ja.yhcePhkh.html
信州大学研究者総覧へのリンク
氏名 Shimosato, Takeshi
URL http://soar-rd.shinshu-u.ac.jp/profile/ja.ONLCjFkV.html
出版者
出版者 SPRINGER
引用
内容記述タイプ Other
内容記述 APPLIED MICROBIOLOGY AND BIOTECHNOLOGY. 101(1):341-349 (2017)
書誌情報 APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

巻 101, 号 1, p. 341-349, 発行日 2017-01
内容記述
内容記述タイプ Other
内容記述 Epub 2016 Oct 8.
抄録
内容記述タイプ Abstract
内容記述 Interleukin 6 (IL-6) is an important pathogenic factor in development of various inflammatory and autoimmune diseases and cancer. Blocking antibodies against molecules associated with IL-6/IL-6 receptor signaling are an attractive candidate for the prevention or therapy of these diseases. In this study, we developed a genetically modified strain of Lactococcus lactis secreting a single-chain variable fragment antibody against mouse IL-6 (IL6scFv). An IL6scFv-secretion vector was constructed by cloning an IL6scFv gene fragment into a lactococcal secretion plasmid and was electroporated into L. lactis NZ9000 (NZ-IL6scFv). Secretion of recombinant IL6scFv (rIL6scFv) by nisin-induced NZ-IL6scFv was confirmed by western blotting and was optimized by tuning culture conditions. We found that rIL6scFv could bind to commercial recombinant mouse IL-6. This result clearly demonstrated the immunoreactivity of rIL6scFv. This is the first study to engineer a genetically modified strain of lactic acid bacteria (gmLAB) that produces a functional anti-cytokine scFv. Numerous previous studies suggested that mucosal delivery of biomedical proteins using gmLAB is an effective and low-cost way to treat various disorders. Therefore, NZ-IL6scFv may be an attractive tool for the research and development of new IL-6 targeting agents for various inflammatory and autoimmune diseases as well as for cancer.
資源タイプ(コンテンツの種類)
内容記述タイプ Other
内容記述 Article
ISSN
収録物識別子タイプ ISSN
収録物識別子 0175-7598
書誌レコードID
収録物識別子タイプ NCID
収録物識別子 AA10503386
PubMed
関連識別子
識別子タイプ PMID
関連識別子 http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?CMD=search&DB=pubmed&term=27722916
関連名称
関連名称 27722916
DOI
関連識別子
識別子タイプ DOI
関連識別子 https://doi.org/10.1007/s00253-016-7907-8
関連名称
関連名称 10.1007/s00253-016-7907-8
権利
権利情報 The original publication is available at www.springerlink.com
出版タイプ
出版タイプ AM
出版タイプResource http://purl.org/coar/version/c_ab4af688f83e57aa
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