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Purification, molecular cloning and functional characterization of flavonoid C-glucosyltransferases from Fagopyrum esculentum M. (buckwheat) cotyledon
http://hdl.handle.net/10091/00019714
http://hdl.handle.net/10091/000197149c4bcb7c-8955-4e7a-9e47-fa5416393517
| 名前 / ファイル | ライセンス | アクション |
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Purification_molecular_cloning_functional_characterization.pdf (9.7 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2017-05-23 | |||||
| タイトル | ||||||
| タイトル | Purification, molecular cloning and functional characterization of flavonoid C-glucosyltransferases from Fagopyrum esculentum M. (buckwheat) cotyledon | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | https://doi.org/10.1111/tpj.12645 | |||||
| 関連名称 | 10.1111/tpj.12645 | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | flavonoid C-glucosyltransferase | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | buckwheat | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Fagopyrum esculentum | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | cotyledon | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | 2-hydroxyflavanone | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | germination | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | vitexin | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| 著者 |
Nagatomo, Yoshihisa
× Nagatomo, Yoshihisa× Usui, Shiori× Ito, Takamitsu× Kato, Akira× Shimosaka, Makoto× Taguchi, Goro |
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| 信州大学研究者総覧へのリンク | ||||||
| 表示名 | Shimosaka, Makoto | |||||
| URL | http://soar-rd.shinshu-u.ac.jp/profile/ja.ZekmjekV.html | |||||
| 信州大学研究者総覧へのリンク | ||||||
| 表示名 | Taguchi, Goro | |||||
| URL | http://soar-rd.shinshu-u.ac.jp/profile/ja.uCDpjekV.html | |||||
| 出版者 | ||||||
| 出版者 | WILEY-BLACKWELL | |||||
| 引用 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | PLANT JOURNAL. 80(3):437-448 (2014) | |||||
| 書誌情報 |
PLANT JOURNAL 巻 80, 号 3, p. 437-448, 発行日 2014-11 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | C-Glycosides are characterized by their C-C bonds in which the anomeric carbon of the sugar moieties is directly bound to the carbon atom of aglycon. C-Glycosides are remarkably stable, as their C-C bonds are resistant to glycosidase or acid hydrolysis. A variety of plant species are known to accumulate C-glycosylflavonoids | |||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | however, the genes encoding for enzymes that catalyze C-glycosylation of flavonoids have been identified only from Oryza sativa (rice) and Zea mays (maize), and have not been identified from dicot plants. In this study, we identified the C-glucosyltransferase gene from the dicot plant Fagopyrum esculentumM. (buckwheat). We purified two isozymes from buckwheat seedlings that catalyze C-glucosylation of 2-hydroxyflavanones, which are expressed specifically in the cotyledon during seed germination. Following purification we isolated the cDNA corresponding to each isozyme [FeCGTa (UGT708C1) and FeCGTb (UGT708C2)]. When expressed in Escherichia coli, both proteins demonstrated C-glucosylation activity towards 2-hydroxyflavanones, dihydrochalcone, trihydroxyacetophenones and other related compounds with chemical structures similar to 2,4,6-trihydroxyacetophenone. Molecular phylogenetic analysis of plant glycosyltransferases shows that flavonoid C-glycosyltransferases form a different clade with other functionally analyzed plant glycosyltransferases. | |||||
| 資源タイプ（コンテンツの種類） | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Article | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0960-7412 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA10846815 | |||||
| PubMed | ||||||
| 識別子タイプ | PMID | |||||
| 関連識別子 | http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?CMD=search&DB=pubmed&term=25142187 | |||||
| 関連名称 | 25142187 | |||||
| 権利 | ||||||
| 権利情報 | This is the peer reviewed version of the following article: Nagatomo, Y., Usui, S., Ito, T., Kato, A., Shimosaka, M. and Taguchi, G. (2014), Purification, molecular cloning and functional characterization of flavonoid C-glucosyltransferases from Fagopyrum esculentum M. (buckwheat) cotyledon. Plant J, 80: 437–448., which has been published in final form at https://doi.org/10.1111/tpj.12645 . This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving. | |||||
| 出版タイプ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| WoS | ||||||
| 表示名 | Web of Science | |||||
| URL | http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=ShinshuUniv&SrcApp=ShinshuUniv&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000343877900005 | |||||
