{"_buckets": {"deposit": "029e3ae9-6bad-45af-99e9-974588bf993e"}, "_deposit": {"id": "18952", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "18952"}, "status": "published"}, "_oai": {"id": "oai:soar-ir.repo.nii.ac.jp:00018952", "sets": ["1310"]}, "author_link": ["103680", "103681", "103682", "103683", "103684", "103685"], "item_1628147817048": {"attribute_name": "出版タイプ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_970fb48d4fbd8a85", "subitem_version_type": "VoR"}]}, "item_6_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2014-11", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "3", "bibliographicPageEnd": "448", "bibliographicPageStart": "437", "bibliographicVolumeNumber": "80", "bibliographic_titles": [{"bibliographic_title": "PLANT JOURNAL"}]}]}, "item_6_description_20": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "C-Glycosides are characterized by their C-C bonds in which the anomeric carbon of the sugar moieties is directly bound to the carbon atom of aglycon. C-Glycosides are remarkably stable, as their C-C bonds are resistant to glycosidase or acid hydrolysis. A variety of plant species are known to accumulate C-glycosylflavonoids", "subitem_description_type": "Abstract"}, {"subitem_description": "however, the genes encoding for enzymes that catalyze C-glycosylation of flavonoids have been identified only from Oryza sativa (rice) and Zea mays (maize), and have not been identified from dicot plants. In this study, we identified the C-glucosyltransferase gene from the dicot plant Fagopyrum esculentumM. (buckwheat). We purified two isozymes from buckwheat seedlings that catalyze C-glucosylation of 2-hydroxyflavanones, which are expressed specifically in the cotyledon during seed germination. Following purification we isolated the cDNA corresponding to each isozyme [FeCGTa (UGT708C1) and FeCGTb (UGT708C2)]. When expressed in Escherichia coli, both proteins demonstrated C-glucosylation activity towards 2-hydroxyflavanones, dihydrochalcone, trihydroxyacetophenones and other related compounds with chemical structures similar to 2,4,6-trihydroxyacetophenone. Molecular phylogenetic analysis of plant glycosyltransferases shows that flavonoid C-glycosyltransferases form a different clade with other functionally analyzed plant glycosyltransferases.", "subitem_description_type": "Abstract"}]}, "item_6_description_30": {"attribute_name": "資源タイプ（コンテンツの種類）", "attribute_value_mlt": [{"subitem_description": "Article", "subitem_description_type": "Other"}]}, "item_6_description_5": {"attribute_name": "引用", "attribute_value_mlt": [{"subitem_description": "PLANT JOURNAL. 80(3):437-448 (2014)", "subitem_description_type": "Other"}]}, "item_6_link_3": {"attribute_name": "信州大学研究者総覧へのリンク", "attribute_value_mlt": [{"subitem_link_text": "Shimosaka, Makoto", "subitem_link_url": "http://soar-rd.shinshu-u.ac.jp/profile/ja.ZekmjekV.html"}, {"subitem_link_text": "Taguchi, Goro", "subitem_link_url": "http://soar-rd.shinshu-u.ac.jp/profile/ja.uCDpjekV.html"}]}, "item_6_link_67": {"attribute_name": "WoS", "attribute_value_mlt": [{"subitem_link_text": "Web of Science", "subitem_link_url": "http://gateway.isiknowledge.com/gateway/Gateway.cgi?\u0026GWVersion=2\u0026SrcAuth=ShinshuUniv\u0026SrcApp=ShinshuUniv\u0026DestLinkType=FullRecord\u0026DestApp=WOS\u0026KeyUT=000343877900005"}]}, "item_6_publisher_4": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "WILEY-BLACKWELL"}]}, "item_6_relation_47": {"attribute_name": "PubMed", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "25142187"}], "subitem_relation_type_id": {"subitem_relation_type_id_text": "http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?CMD=search\u0026DB=pubmed\u0026term=25142187", "subitem_relation_type_select": "PMID"}}]}, "item_6_relation_48": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "10.1111/tpj.12645"}], "subitem_relation_type_id": {"subitem_relation_type_id_text": "https://doi.org/10.1111/tpj.12645", "subitem_relation_type_select": "DOI"}}]}, "item_6_rights_62": {"attribute_name": "権利", "attribute_value_mlt": [{"subitem_rights": "This is the peer reviewed version of the following article: Nagatomo, Y., Usui, S., Ito, T., Kato, A., Shimosaka, M. and Taguchi, G. (2014), Purification, molecular cloning and functional characterization of flavonoid C-glucosyltransferases from Fagopyrum esculentum M. (buckwheat) cotyledon. Plant J, 80: 437–448., which has been published in final form at https://doi.org/10.1111/tpj.12645 . This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving."}]}, "item_6_select_64": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_select_item": "publisher"}]}, "item_6_source_id_35": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "0960-7412", "subitem_source_identifier_type": "ISSN"}]}, "item_6_source_id_39": {"attribute_name": "NII ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "0960-7412", "subitem_source_identifier_type": "ISSN"}]}, "item_6_source_id_40": {"attribute_name": "書誌レコードID", "attribute_value_mlt": [{"subitem_source_identifier": "AA10846815", "subitem_source_identifier_type": "NCID"}]}, "item_6_text_69": {"attribute_name": "wosonly authkey", "attribute_value_mlt": [{"subitem_text_value": "flavonoid C-glucosyltransferase@@@buckwheat@@@Fagopyrum esculentum@@@cotyledon@@@2-hydroxyflavanone@@@germination@@@vitexin"}]}, "item_6_text_70": {"attribute_name": "wosonly keywords", "attribute_value_mlt": [{"subitem_text_value": "ANTIBIOTIC URDAMYCIN@@@SECONDARY METABOLISM@@@TOBACCO CELLS@@@GLYCOSYLTRANSFERASE@@@BIOSYNTHESIS@@@VITEXIN@@@GLUCOSIDES@@@URDGT2@@@ANTIOXIDANT@@@GLYCOSIDES"}]}, "item_6_textarea_68": {"attribute_name": "wosonly abstract", "attribute_value_mlt": [{"subitem_textarea_value": "C-Glycosides are characterized by their C-C bonds in which the anomeric carbon of the sugar moieties is directly bound to the carbon atom of aglycon. C-Glycosides are remarkably stable, as their C-C bonds are resistant to glycosidase or acid hydrolysis. A variety of plant species are known to accumulate C-glycosylflavonoids@@@however, the genes encoding for enzymes that catalyze C-glycosylation of flavonoids have been identified only from Oryza sativa (rice) and Zea mays (maize), and have not been identified from dicot plants. In this study, we identified the C-glucosyltransferase gene from the dicot plant Fagopyrum esculentumM. (buckwheat). We purified two isozymes from buckwheat seedlings that catalyze C-glucosylation of 2-hydroxyflavanones, which are expressed specifically in the cotyledon during seed germination. Following purification we isolated the cDNA corresponding to each isozyme [FeCGTa (UGT708C1) and FeCGTb (UGT708C2)]. When expressed in Escherichia coli, both proteins demonstrated C-glucosylation activity towards 2-hydroxyflavanones, dihydrochalcone, trihydroxyacetophenones and other related compounds with chemical structures similar to 2,4,6-trihydroxyacetophenone. Molecular phylogenetic analysis of plant glycosyltransferases shows that flavonoid C-glycosyltransferases form a different clade with other functionally analyzed plant glycosyltransferases."}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Nagatomo, Yoshihisa"}], "nameIdentifiers": [{"nameIdentifier": "103680", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Usui, Shiori"}], "nameIdentifiers": [{"nameIdentifier": "103681", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Ito, Takamitsu"}], "nameIdentifiers": [{"nameIdentifier": "103682", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kato, Akira"}], "nameIdentifiers": [{"nameIdentifier": "103683", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Shimosaka, Makoto"}], "nameIdentifiers": [{"nameIdentifier": "103684", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Taguchi, Goro"}], "nameIdentifiers": [{"nameIdentifier": "103685", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2017-05-23"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "Purification_molecular_cloning_functional_characterization.pdf", "filesize": [{"value": "9.7 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensefree": "This is the peer reviewed version of the following article: Nagatomo, Y., Usui, S., Ito, T., Kato, A., Shimosaka, M. and Taguchi, G. (2014), Purification, molecular cloning and functional characterization of flavonoid C-glucosyltransferases from Fagopyrum esculentum M. (buckwheat) cotyledon. Plant J, 80: 437–448., which has been published in final form at https://doi.org/10.1111/tpj.12645 . This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.", "licensetype": "license_note", "mimetype": "application/pdf", "size": 9700000.0, "url": {"label": "Purification_molecular_cloning_functional_characterization.pdf", "url": "https://soar-ir.repo.nii.ac.jp/record/18952/files/Purification_molecular_cloning_functional_characterization.pdf"}, "version_id": "559d451e-ea03-45b5-ae04-23a00cc8bee2"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "flavonoid C-glucosyltransferase", "subitem_subject_scheme": "Other"}, {"subitem_subject": "buckwheat", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Fagopyrum esculentum", "subitem_subject_scheme": "Other"}, {"subitem_subject": "cotyledon", "subitem_subject_scheme": "Other"}, {"subitem_subject": "2-hydroxyflavanone", "subitem_subject_scheme": "Other"}, {"subitem_subject": "germination", "subitem_subject_scheme": "Other"}, {"subitem_subject": "vitexin", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Purification, molecular cloning and functional characterization of flavonoid C-glucosyltransferases from Fagopyrum esculentum M. (buckwheat) cotyledon", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Purification, molecular cloning and functional characterization of flavonoid C-glucosyltransferases from Fagopyrum esculentum M. (buckwheat) cotyledon", "subitem_title_language": "en"}]}, "item_type_id": "6", "owner": "1", "path": ["1310"], "permalink_uri": "http://hdl.handle.net/10091/00019714", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2017-05-23"}, "publish_date": "2017-05-23", "publish_status": "0", "recid": "18952", "relation": {}, "relation_version_is_last": true, "title": ["Purification, molecular cloning and functional characterization of flavonoid C-glucosyltransferases from Fagopyrum esculentum M. (buckwheat) cotyledon"], "weko_shared_id": -1}