Item type |
学術雑誌論文 / Journal Article(1) |
公開日 |
2017-05-23 |
タイトル |
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タイトル |
Purification, molecular cloning and functional characterization of flavonoid C-glucosyltransferases from Fagopyrum esculentum M. (buckwheat) cotyledon |
言語 |
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言語 |
eng |
DOI |
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関連識別子 |
https://doi.org/10.1111/tpj.12645 |
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関連名称 |
10.1111/tpj.12645 |
キーワード |
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主題 |
flavonoid C-glucosyltransferase, buckwheat, Fagopyrum esculentum, cotyledon, 2-hydroxyflavanone, germination, vitexin |
資源タイプ |
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資源 |
http://purl.org/coar/resource_type/c_6501 |
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タイプ |
journal article |
著者 |
Nagatomo, Yoshihisa
Usui, Shiori
Ito, Takamitsu
Kato, Akira
Shimosaka, Makoto
Taguchi, Goro
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信州大学研究者総覧へのリンク |
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氏名 |
Shimosaka, Makoto |
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URL |
http://soar-rd.shinshu-u.ac.jp/profile/ja.ZekmjekV.html |
信州大学研究者総覧へのリンク |
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氏名 |
Taguchi, Goro |
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URL |
http://soar-rd.shinshu-u.ac.jp/profile/ja.uCDpjekV.html |
出版者 |
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出版者 |
WILEY-BLACKWELL |
引用 |
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内容記述 |
PLANT JOURNAL. 80(3):437-448 (2014) |
書誌情報 |
PLANT JOURNAL
巻 80,
号 3,
p. 437-448,
発行日 2014-11
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抄録 |
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内容記述 |
C-Glycosides are characterized by their C-C bonds in which the anomeric carbon of the sugar moieties is directly bound to the carbon atom of aglycon. C-Glycosides are remarkably stable, as their C-C bonds are resistant to glycosidase or acid hydrolysis. A variety of plant species are known to accumulate C-glycosylflavonoids |
抄録 |
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内容記述 |
however, the genes encoding for enzymes that catalyze C-glycosylation of flavonoids have been identified only from Oryza sativa (rice) and Zea mays (maize), and have not been identified from dicot plants. In this study, we identified the C-glucosyltransferase gene from the dicot plant Fagopyrum esculentumM. (buckwheat). We purified two isozymes from buckwheat seedlings that catalyze C-glucosylation of 2-hydroxyflavanones, which are expressed specifically in the cotyledon during seed germination. Following purification we isolated the cDNA corresponding to each isozyme [FeCGTa (UGT708C1) and FeCGTb (UGT708C2)]. When expressed in Escherichia coli, both proteins demonstrated C-glucosylation activity towards 2-hydroxyflavanones, dihydrochalcone, trihydroxyacetophenones and other related compounds with chemical structures similar to 2,4,6-trihydroxyacetophenone. Molecular phylogenetic analysis of plant glycosyltransferases shows that flavonoid C-glycosyltransferases form a different clade with other functionally analyzed plant glycosyltransferases. |
資源タイプ(コンテンツの種類) |
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内容記述 |
Article |
ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
0960-7412 |
書誌レコードID |
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収録物識別子タイプ |
NCID |
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収録物識別子 |
AA10846815 |
PubMed |
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関連識別子 |
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?CMD=search&DB=pubmed&term=25142187 |
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関連名称 |
25142187 |
権利 |
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権利情報 |
This is the peer reviewed version of the following article: Nagatomo, Y., Usui, S., Ito, T., Kato, A., Shimosaka, M. and Taguchi, G. (2014), Purification, molecular cloning and functional characterization of flavonoid C-glucosyltransferases from Fagopyrum esculentum M. (buckwheat) cotyledon. Plant J, 80: 437–448., which has been published in final form at https://doi.org/10.1111/tpj.12645 . This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving. |
出版タイプ |
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出版タイプ |
VoR |
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出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
WoS |
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URL |
http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=ShinshuUniv&SrcApp=ShinshuUniv&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000343877900005 |