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  1. 050 医学部, 大学院医学系研究科
  2. 0501 学術論文

Evaluation of MC3T3-E1 Cell Osteogenesis in Different Cell Culture Media

http://hdl.handle.net/10091/0002000345
fcfd6e45-f9c1-4b3c-a2f7-9ac128389a7c
名前 / ファイル ライセンス アクション
ijms-22-07752-v3.pdf ijms-22-07752-v3.pdf (4.7 MB)
license.icon
Item type 学術雑誌論文 / Journal Article(1)
公開日 2021-07-28
タイトル
言語 en
タイトル Evaluation of MC3T3-E1 Cell Osteogenesis in Different Cell Culture Media
言語
言語 eng
キーワード
言語 en
主題Scheme Other
主題 biomaterials
キーワード
言語 en
主題Scheme Other
主題 vitamin C
キーワード
言語 en
主題Scheme Other
主題 osteoblast
キーワード
言語 en
主題Scheme Other
主題 calcification
キーワード
言語 en
主題Scheme Other
主題 cell culture
キーワード
言語 en
主題Scheme Other
主題 collagen
キーワード
言語 en
主題Scheme Other
主題 alkaline phosphatase
資源タイプ
資源 http://purl.org/coar/resource_type/c_6501
タイプ journal article
著者 Izumiya, Makoto

× Izumiya, Makoto

en Izumiya, Makoto

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Haniu, Miyu

× Haniu, Miyu

en Haniu, Miyu

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Ueda, Katsuya

× Ueda, Katsuya

en Ueda, Katsuya

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Ishida, Haruka

× Ishida, Haruka

en Ishida, Haruka

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Ma, Chuang

× Ma, Chuang

en Ma, Chuang

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Ideta, Hirokazu

× Ideta, Hirokazu

en Ideta, Hirokazu

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Sobajima, Atsushi

× Sobajima, Atsushi

en Sobajima, Atsushi

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Ueshiba, Koki

× Ueshiba, Koki

en Ueshiba, Koki

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Uemura, Takeshi

× Uemura, Takeshi

en Uemura, Takeshi

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Saito, Naoto

× Saito, Naoto

en Saito, Naoto

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Haniu, Hisao

× Haniu, Hisao

en Haniu, Hisao

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信州大学研究者総覧へのリンク
氏名 Sobajima Atsushi
URL https://soar-rd.shinshu-u.ac.jp/profile/ja.uULePpym.html
信州大学研究者総覧へのリンク
氏名 Uemura Takeshi
URL https://soar-rd.shinshu-u.ac.jp/profile/ja.HmANWayV.html
信州大学研究者総覧へのリンク
氏名 Saito Naoto
URL https://soar-rd.shinshu-u.ac.jp/profile/ja.WpkhuUkh.html
信州大学研究者総覧へのリンク
氏名 Haniu, Hisao
URL https://soar-rd.shinshu-u.ac.jp/profile/ja.jakpPmSa.html
出版者
出版者 MDPI
引用
内容記述タイプ Other
内容記述 International Journal of Molecular Sciences. 22(14):7752 (2021)
書誌情報 International Journal of Molecular Sciences

巻 22, 号 14, p. 7752, 発行日 2021-07-20
抄録
内容記述タイプ Abstract
内容記述 Many biomaterials have been evaluated using cultured cells. In particular, osteoblast-like cells are often used to evaluate the osteocompatibility, hard-tissue-regeneration, osteoconductive, and osteoinductive characteristics of biomaterials. However, the evaluation of biomaterial osteogenesis-inducing capacity using osteoblast-like cells is not standardized; instead, it is performed under laboratory-specific culture conditions with different culture media. However, the effect of different media conditions on bone formation has not been investigated. Here, we aimed to evaluate the osteogenesis of MC3T3-E1 cells, one of the most commonly used osteoblast-like cell lines for osteogenesis evaluation, and assayed cell proliferation, alkaline phosphatase activity, expression of osteoblast markers, and calcification under varying culture media conditions. Furthermore, the various media conditions were tested in uncoated plates and plates coated with collagen type I and poly-L-lysine, highly biocompatible molecules commonly used as pseudobiomaterials. We found that the type of base medium, the presence or absence of vitamin C, and the freshness of the medium may affect biomaterial regeneration. We posit that an in vitro model that recapitulates in vivo bone formation should be established before evaluating biomaterials.
言語 en
資源タイプ(コンテンツの種類)
内容記述タイプ Other
内容記述 Article
ISSN
収録物識別子タイプ EISSN
収録物識別子 1422-0067
PubMed
関連識別子
識別子タイプ PMID
関連識別子 https://pubmed.ncbi.nlm.nih.gov/34299372/
関連名称
関連名称 34299372
DOI
関連識別子
識別子タイプ DOI
関連識別子 https://doi.org/10.3390/ijms22147752
関連名称
関連名称 10.3390/ijms22147752
権利
権利情報 © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
出版タイプ
出版タイプ VoR
出版タイプResource http://purl.org/coar/version/c_970fb48d4fbd8a85
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