{"_buckets": {"deposit": "b3d704f8-93d0-436b-8268-d2d23956ecdd"}, "_deposit": {"created_by": 16, "id": "2000388", "owner": "16", "owners": [16], "owners_ext": {"displayname": "shinshu_univ", "username": "shinshu_univ"}, "pid": {"revision_id": 0, "type": "depid", "value": "2000388"}, "status": "published"}, "_oai": {"id": "oai:soar-ir.repo.nii.ac.jp:02000388", "sets": ["1309:1310"]}, "author_link": [], "item_1628147817048": {"attribute_name": "\u51fa\u7248\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_ab4af688f83e57aa", "subitem_version_type": "AM"}]}, "item_6_biblio_info_6": {"attribute_name": "\u66f8\u8a8c\u60c5\u5831", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2018-10-03", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "10", "bibliographicPageEnd": "1802", "bibliographicPageStart": "1790", "bibliographicVolumeNumber": "82", "bibliographic_titles": [{"bibliographic_title": "Bioscience, Biotechnology, and Biochemistry"}]}]}, "item_6_description_20": {"attribute_name": "\u6284\u9332", "attribute_value_mlt": [{"subitem_description": "Rutin, a 3-rutinosyl quercetin, is a representative flavonoid distributed in many plant species, and is highlighted for its therapeutic potential. In this study, we purified uridine diphosphate-rhamnose: quercetin 3-O-glucoside 6\u2033-O-rhamnosyltransferase and isolated the corresponding cDNA (FeF3G6\u2033RhaT) from seedlings of common buckwheat (Fagopyrum esculentum). The recombinant FeF3G6\u2033RhaT enzyme expressed in Escherichia coli exhibited 6\u2033-O-rhamnosylation activity against flavonol 3-O-glucoside and flavonol 3-O-galactoside as substrates, but showed only faint activity against flavonoid 7-O-glucosides. Tobacco cells expressing FeF3G6\u2033RhaT converted the administered quercetin into rutin, suggesting that FeF3G6\u2033RhaT can function as a rhamnosyltransferase in planta. Quantitative PCR analysis on several organs of common buckwheat revealed that accumulation of FeF3G6\u2033RhaT began during the early developmental stages of rutin-accumulating organs, such as flowers, leaves, and cotyledons. These results suggest that FeF3G6\u2033RhaT is involved in rutin biosynthesis in common buckwheat.", "subitem_description_type": "Abstract"}]}, "item_6_description_5": {"attribute_name": "\u5f15\u7528", "attribute_value_mlt": [{"subitem_description": "Bioscience, Biotechnology, and Biochemistry. 82(10):1790\u20131802(2018)", "subitem_description_type": "Other"}]}, "item_6_link_3": {"attribute_name": "\u4fe1\u5dde\u5927\u5b66\u7814\u7a76\u8005\u7dcf\u89a7\u3078\u306e\u30ea\u30f3\u30af", "attribute_value_mlt": [{"subitem_link_text": "\u4e0b\u5742, \u8aa0", "subitem_link_url": "https://soar-rd.shinshu-u.ac.jp/profile/ja.ZekmjekV.html"}, {"subitem_link_text": "\u7530\u53e3, \u609f\u6717", "subitem_link_url": "https://soar-rd.shinshu-u.ac.jp/profile/ja.uCDpjekV.html"}]}, "item_6_publisher_4": {"attribute_name": "\u51fa\u7248\u8005", "attribute_value_mlt": [{"subitem_publisher": "Japan Society for Bioscience, Biotechnology, and Agrochemistry"}]}, "item_6_relation_47": {"attribute_name": "PubMed", "attribute_value_mlt": [{"subitem_relation_type_id": {"subitem_relation_type_id_text": "https://pubmed.ncbi.nlm.nih.gov/29972345", "subitem_relation_type_select": "PMID"}}]}, "item_6_relation_48": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type": "isVersionOf", "subitem_relation_type_id": {"subitem_relation_type_id_text": "https://doi.org/10.1080/09168451.2018.1491286", "subitem_relation_type_select": "DOI"}}]}, "item_6_select_64": {"attribute_name": "\u8457\u8005\u7248\u30d5\u30e9\u30b0", "attribute_value_mlt": [{"subitem_select_item": "author"}]}, "item_6_source_id_35": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "1347-6947", "subitem_source_identifier_type": "EISSN"}]}, "item_6_source_id_39": {"attribute_name": "NII ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "1347-6947", "subitem_source_identifier_type": "EISSN"}]}, "item_6_source_id_40": {"attribute_name": "\u66f8\u8a8c\u30ec\u30b3\u30fc\u30c9ID", "attribute_value_mlt": [{"subitem_source_identifier": "AA12018610", "subitem_source_identifier_type": "NCID"}]}, "item_creator": {"attribute_name": "\u8457\u8005", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Koja, Eiki", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Ohata, Soichiro", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Maruyama, Yoshinori", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Suzuki, Hideyuki", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Shimosaka, Makoto", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Taguchi, Goro", "creatorNameLang": "en"}]}]}, "item_files": {"attribute_name": "\u30d5\u30a1\u30a4\u30eb\u60c5\u5831", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_access", "date": [{"dateType": "Available", "dateValue": "2021-09-28"}], "download_preview_message": "", "file_order": 0, "filename": "1491286.pdf", "filesize": [{"value": "2.9 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_5", "mimetype": "application/pdf", "size": 2900000.0, "url": {"url": "https://soar-ir.repo.nii.ac.jp/record/2000388/files/1491286.pdf"}, "version_id": "eb7a775f-cc2e-46d9-a7fa-cc63cb726c3f"}]}, "item_keyword": {"attribute_name": "\u30ad\u30fc\u30ef\u30fc\u30c9", "attribute_value_mlt": [{"subitem_subject": "common buckwheat", "subitem_subject_scheme": "Other"}, {"subitem_subject": "flavonoid biosynthesis", "subitem_subject_scheme": "Other"}, {"subitem_subject": "rhamnosyltransferase", "subitem_subject_scheme": "Other"}, {"subitem_subject": "rutin", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "\u8a00\u8a9e", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Identification and characterization of a rhamnosyltransferase involved in rutin biosynthesis in Fagopyrum esculentum (common buckwheat)", "item_titles": {"attribute_name": "\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_title": "Identification and characterization of a rhamnosyltransferase involved in rutin biosynthesis in Fagopyrum esculentum (common buckwheat)", "subitem_title_language": "en"}]}, "item_type_id": "6", "owner": "16", "path": ["1309/1310"], "permalink_uri": "http://hdl.handle.net/10091/0002000388", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2021-09-29"}, "publish_date": "2021-09-29", "publish_status": "0", "recid": "2000388", "relation": {}, "relation_version_is_last": true, "title": ["Identification and characterization of a rhamnosyltransferase involved in rutin biosynthesis in Fagopyrum esculentum (common buckwheat)"], "weko_shared_id": -1}