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Identification and characterization of a rhamnosyltransferase involved in rutin biosynthesis in Fagopyrum esculentum (common buckwheat)
http://hdl.handle.net/10091/0002000388
http://hdl.handle.net/10091/00020003883a5f0142-f804-4344-ac1b-482492d36fc3
| 名前 / ファイル | ライセンス | アクション |
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1491286.pdf (2.9 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||||||||||||||
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| 公開日 | 2021-09-29 | |||||||||||||||||
| タイトル | ||||||||||||||||||
| タイトル | Identification and characterization of a rhamnosyltransferase involved in rutin biosynthesis in Fagopyrum esculentum (common buckwheat) | |||||||||||||||||
| 言語 | ||||||||||||||||||
| 言語 | eng | |||||||||||||||||
| DOI | ||||||||||||||||||
| 関連タイプ | isVersionOf | |||||||||||||||||
| 関連識別子 | https://doi.org/10.1080/09168451.2018.1491286 | |||||||||||||||||
| キーワード | ||||||||||||||||||
| 主題 | common buckwheat, flavonoid biosynthesis, rhamnosyltransferase, rutin | |||||||||||||||||
| 資源タイプ | ||||||||||||||||||
| 資源 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||||||
| タイプ | journal article | |||||||||||||||||
| 著者 |
Koja, Eiki
× Koja, Eiki
× Ohata, Soichiro
× Maruyama, Yoshinori
× Suzuki, Hideyuki
× Shimosaka, Makoto
× Taguchi, Goro
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| 信州大学研究者総覧へのリンク | ||||||||||||||||||
| 氏名 | 下坂, 誠 | |||||||||||||||||
| URL | https://soar-rd.shinshu-u.ac.jp/profile/ja.ZekmjekV.html | |||||||||||||||||
| 信州大学研究者総覧へのリンク | ||||||||||||||||||
| 氏名 | 田口, 悟朗 | |||||||||||||||||
| URL | https://soar-rd.shinshu-u.ac.jp/profile/ja.uCDpjekV.html | |||||||||||||||||
| 出版者 | ||||||||||||||||||
| 出版者 | Japan Society for Bioscience, Biotechnology, and Agrochemistry | |||||||||||||||||
| 引用 | ||||||||||||||||||
| 内容記述 | Bioscience, Biotechnology, and Biochemistry. 82(10):1790–1802(2018) | |||||||||||||||||
| 書誌情報 |
Bioscience, Biotechnology, and Biochemistry 巻 82, 号 10, p. 1790-1802, 発行日 2018-10-03 |
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| 抄録 | ||||||||||||||||||
| 内容記述 | Rutin, a 3-rutinosyl quercetin, is a representative flavonoid distributed in many plant species, and is highlighted for its therapeutic potential. In this study, we purified uridine diphosphate-rhamnose: quercetin 3-O-glucoside 6″-O-rhamnosyltransferase and isolated the corresponding cDNA (FeF3G6″RhaT) from seedlings of common buckwheat (Fagopyrum esculentum). The recombinant FeF3G6″RhaT enzyme expressed in Escherichia coli exhibited 6″-O-rhamnosylation activity against flavonol 3-O-glucoside and flavonol 3-O-galactoside as substrates, but showed only faint activity against flavonoid 7-O-glucosides. Tobacco cells expressing FeF3G6″RhaT converted the administered quercetin into rutin, suggesting that FeF3G6″RhaT can function as a rhamnosyltransferase in planta. Quantitative PCR analysis on several organs of common buckwheat revealed that accumulation of FeF3G6″RhaT began during the early developmental stages of rutin-accumulating organs, such as flowers, leaves, and cotyledons. These results suggest that FeF3G6″RhaT is involved in rutin biosynthesis in common buckwheat. | |||||||||||||||||
| ISSN | ||||||||||||||||||
| 収録物識別子タイプ | EISSN | |||||||||||||||||
| 収録物識別子 | 1347-6947 | |||||||||||||||||
| 書誌レコードID | ||||||||||||||||||
| 収録物識別子タイプ | NCID | |||||||||||||||||
| 収録物識別子 | AA12018610 | |||||||||||||||||
| PubMed | ||||||||||||||||||
| 識別子タイプ | PMID | |||||||||||||||||
| 関連識別子 | https://pubmed.ncbi.nlm.nih.gov/29972345 | |||||||||||||||||
| 出版タイプ | ||||||||||||||||||
| 出版タイプ | AM | |||||||||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||||||||||||||
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Cite as
Koja, Eiki, Ohata, Soichiro, Maruyama, Yoshinori, Suzuki, Hideyuki, Shimosaka, Makoto, Taguchi, Goro, 2018, Identification and characterization of a rhamnosyltransferase involved in rutin biosynthesis in Fagopyrum esculentum (common buckwheat): Japan Society for Bioscience, Biotechnology, and Agrochemistry, 1790–1802 p.
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