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  1. 080 繊維学部
  2. 0801 学術論文

Identification and characterization of a rhamnosyltransferase involved in rutin biosynthesis in Fagopyrum esculentum (common buckwheat)

http://hdl.handle.net/10091/0002000388
http://hdl.handle.net/10091/0002000388
3a5f0142-f804-4344-ac1b-482492d36fc3
名前 / ファイル ライセンス アクション
1491286.pdf 1491286.pdf (2.9 MB)
license.icon
Item type 学術雑誌論文 / Journal Article(1)
公開日 2021-09-29
タイトル
言語 en
タイトル Identification and characterization of a rhamnosyltransferase involved in rutin biosynthesis in Fagopyrum esculentum (common buckwheat)
言語
言語 eng
キーワード
主題Scheme Other
主題 common buckwheat
キーワード
主題Scheme Other
主題 flavonoid biosynthesis
キーワード
主題Scheme Other
主題 rhamnosyltransferase
キーワード
主題Scheme Other
主題 rutin
資源タイプ
資源 http://purl.org/coar/resource_type/c_6501
タイプ journal article
著者 Koja, Eiki

× Koja, Eiki

en Koja, Eiki

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Ohata, Soichiro

× Ohata, Soichiro

en Ohata, Soichiro

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Maruyama, Yoshinori

× Maruyama, Yoshinori

en Maruyama, Yoshinori

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Suzuki, Hideyuki

× Suzuki, Hideyuki

en Suzuki, Hideyuki

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Shimosaka, Makoto

× Shimosaka, Makoto

en Shimosaka, Makoto

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Taguchi, Goro

× Taguchi, Goro

en Taguchi, Goro

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信州大学研究者総覧へのリンク
氏名 下坂, 誠
URL https://soar-rd.shinshu-u.ac.jp/profile/ja.ZekmjekV.html
信州大学研究者総覧へのリンク
氏名 田口, 悟朗
URL https://soar-rd.shinshu-u.ac.jp/profile/ja.uCDpjekV.html
出版者
出版者 Japan Society for Bioscience, Biotechnology, and Agrochemistry
引用
内容記述タイプ Other
内容記述 Bioscience, Biotechnology, and Biochemistry. 82(10):1790–1802(2018)
書誌情報 Bioscience, Biotechnology, and Biochemistry

巻 82, 号 10, p. 1790-1802, 発行日 2018-10-03
抄録
内容記述タイプ Abstract
内容記述 Rutin, a 3-rutinosyl quercetin, is a representative flavonoid distributed in many plant species, and is highlighted for its therapeutic potential. In this study, we purified uridine diphosphate-rhamnose: quercetin 3-O-glucoside 6″-O-rhamnosyltransferase and isolated the corresponding cDNA (FeF3G6″RhaT) from seedlings of common buckwheat (Fagopyrum esculentum). The recombinant FeF3G6″RhaT enzyme expressed in Escherichia coli exhibited 6″-O-rhamnosylation activity against flavonol 3-O-glucoside and flavonol 3-O-galactoside as substrates, but showed only faint activity against flavonoid 7-O-glucosides. Tobacco cells expressing FeF3G6″RhaT converted the administered quercetin into rutin, suggesting that FeF3G6″RhaT can function as a rhamnosyltransferase in planta. Quantitative PCR analysis on several organs of common buckwheat revealed that accumulation of FeF3G6″RhaT began during the early developmental stages of rutin-accumulating organs, such as flowers, leaves, and cotyledons. These results suggest that FeF3G6″RhaT is involved in rutin biosynthesis in common buckwheat.
ISSN
収録物識別子タイプ EISSN
収録物識別子 1347-6947
書誌レコードID
収録物識別子タイプ NCID
収録物識別子 AA12018610
PubMed
識別子タイプ PMID
関連識別子 https://pubmed.ncbi.nlm.nih.gov/29972345
DOI
関連タイプ isVersionOf
識別子タイプ DOI
関連識別子 https://doi.org/10.1080/09168451.2018.1491286
出版タイプ
出版タイプ AM
出版タイプResource http://purl.org/coar/version/c_ab4af688f83e57aa
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