| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2022-11-14 |
| タイトル |
|
|
タイトル |
Acquisition of mcr-1 and Cocarriage of Virulence Genes in Avian Pathogenic Escherichia coli Isolates from Municipal Wastewater Influents in Japan |
| 言語 |
|
|
言語 |
eng |
| DOI |
|
|
関連タイプ |
isIdenticalTo |
|
|
関連識別子 |
https://doi.org/10.1128/AEM.01661-19 |
|
|
関連名称 |
10.1128/AEM.01661-19 |
| キーワード |
|
|
主題 |
WWTPs, mcr-1, APEC, ColV |
| 資源タイプ |
|
|
資源 |
http://purl.org/coar/resource_type/c_6501 |
|
タイプ |
journal article |
| 著者 |
Hayashi, Wataru
Tanaka, Hayato
Taniguchi, Yui
Iimura, Masaki
Soga, Eiji
Kubo, Ryoichi
Matsuo, Nao
Kawamura, Kumiko
Arakawa, Yoshichika
Nagano, Yukiko
Nagano, Noriyuki
|
| 信州大学研究者総覧へのリンク |
|
|
氏名 |
長野, 由紀子 |
|
URL |
https://soar-rd.shinshu-u.ac.jp/profile/ja.OUAeumyC.html |
| 信州大学研究者総覧へのリンク |
|
|
氏名 |
長野, 則之 |
|
URL |
https://soar-rd.shinshu-u.ac.jp/profile/ja.HULFumkh.html |
| 出版者 |
|
|
出版者 |
American Society for Microbiology |
| 引用 |
|
|
内容記述 |
Applied and Environmental Microbiology 85(22) : e01661-19-(2019) |
| 書誌情報 |
Applied and Environmental Microbiology
巻 85,
号 22,
p. e01661-19,
発行日 2019-10-30
|
| 抄録 |
|
|
内容記述 |
This study focused on the detection of the plasmid-mediated mcr colistin resistance gene in Escherichia coli isolates from wastewater treatment plants (WWTPs). Seven influent samples were collected from three WWTPs in Nagano Prefecture, Japan, during August and December 2018. Colistin-resistant E. coli isolates were selected on colistin-supplemented CHROMagar ECC plates. mcr-1-positive isolates were subjected to whole-genome sequencing (WGS) analysis. From six influent samples, seven mcr-1-positive but extended-spectrum beta-lactamase (ESBL)-negative isolates belonging to different genetic lineages, namely, B2-O25:H4-ST131-fimH22, B2-O2:H1-ST135-fimH2, B1-O8:H9-ST764-fimH32, B1-O23:H16-5T453-fimH31, A-O81: H27-ST10-fimH54, A-O16:H5-ST871-fimH25, and F-O11:H6-ST457-fimH145, were detected. The MICs of colistin for these isolates ranged from 4 to 16 mg/liter. The mcr-1 genes were located on plasmids belonging to IncX4 and Incl2 in five and two isolates, respectively. Four IncX4 plasmids with the same size (33,309 bp) showed high sequence similarity (4 single-nucleotide variations). The remaining one IncX4 plasmid, with a size of 33,858 bp, carried the mcr-1 gene with the single synonymous nucleic substitution T27C. Two Incl2 plasmids with sizes of 60,710 bp and 60,733 bp had high sequence similarity (99.9% identity; 100% query coverage). Two of five isolates carrying IncX4 plasmids and both of the isolates carrying Incl2 plasmids harbored CoIV plasmids carrying virulence-associated genes of avian pathogenic E. coli (APEC). In addition, another isolate of the B2-O25:H4-ST131-fimH22 lineage had those APEC-associated virulence genes on its chromosome. In conclusion, mcr-1-positive E. coli environmental isolates were mostly characterized as positive for APEC-associated virulence genes. The copresence of those genes may suggest the existence of a common source in animals and/or their associated environments. |
| 資源タイプ（コンテンツの種類） |
|
| ISSN |
|
|
収録物識別子タイプ |
EISSN |
|
収録物識別子 |
1098-5336 |
| 書誌レコードID |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA00543249 |
| PubMed |
|
|
|
識別子タイプ |
PMID |
|
|
関連識別子 |
https://pubmed.ncbi.nlm.nih.gov/31492666/ |
|
|
関連名称 |
31492666 |
| 権利 |
|
|
権利情報 |
© 2019 American Society for Microbiology. All Rights Reserved. |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
18K08428_2.pdf
