Item type |
学術雑誌論文 / Journal Article(1) |
公開日 |
2022-11-14 |
タイトル |
|
|
タイトル |
Wastewater as a Probable Environmental Reservoir of Extended-Spectrum-β-Lactamase Genes: Detection of Chimeric β-Lactamases CTX-M-64 and CTX-M-123 |
言語 |
|
|
言語 |
eng |
DOI |
|
|
関連タイプ |
isIdenticalTo |
|
|
関連識別子 |
https://doi.org/10.1128/AEM.01740-19 |
|
|
関連名称 |
10.1128/AEM.01740-19 |
キーワード |
|
|
主題 |
wastewater, ESBL, CTX-M-64, CTX-M-123, WWTPs |
資源タイプ |
|
|
資源 |
http://purl.org/coar/resource_type/c_6501 |
|
タイプ |
journal article |
著者 |
Tanaka, Hayato
Hayashi, Wataru
Iimura, Masaki
Taniguchi, Yui
Soga, Eiji
Matsuo, Nao
Kawamura, Kumiko
Arakawa, Yoshichika
Nagano, Yukiko
Nagano, Noriyuki
|
信州大学研究者総覧へのリンク |
|
|
氏名 |
長野, 由紀子 |
|
URL |
https://soar-rd.shinshu-u.ac.jp/profile/ja.OUAeumyC.html |
信州大学研究者総覧へのリンク |
|
|
氏名 |
長野, 則之 |
|
URL |
https://soar-rd.shinshu-u.ac.jp/profile/ja.HULFumkh.html |
出版者 |
|
|
出版者 |
American Society for Microbiology |
引用 |
|
|
内容記述 |
Applied and Environmental Microbiology 85(22) : e01740-19-(2019) |
書誌情報 |
Applied and Environmental Microbiology
巻 85,
号 22,
p. e01740-19,
発行日 2019-10-30
|
抄録 |
|
|
内容記述 |
The presence of antimicrobial-resistant bacteria and resistance genes in aquatic environments is a serious public health concern. This study focused on Escherichia coli possessing bla(CTX-M) genes in wastewater inflows. Twelve crude inflow water samples from wastewater treatment plant (WWTP) A and two samples each from three other WWTPs were collected in 2017 and 2018. A total of 73 E. coli isolates with 31 different sequence types (STs) harboring distinctive bla(CTX-M) gene repertoires were detected. In WWTP A influents, bla(CTX-M-14) (14 isolates) was dominant, followed by bla(CTX-M-15 )(12 isolates) and bla(CTX-M-27) (10 isolates). The chimeric bla(CTX-M-)(64) and bla(CTX-M-123) genes were each identified in one of the E. coli isolates from the same WWTP A inflow port. The bla(CTX-M-27) gene was associated with five of seven B2-ST131 isolates, including three isolates of the B2-O25b-ST131-H30R/non-Rx lineage. One of the remaining two isolates belonged to the B2-O25b-ST131-H30R/Rx lineage harboring the bla(CTX-M-15) gene. As for the B2-025b-ST131-H30R/non-Rx lineage, two isolates with bla(CTX-M-27) were recovered from each of the WWTP B and D influents, and one isolate with bla(CTX-M-174) was also recovered from WWTP B influent. Whole-genome sequencing of chimeric bla(CTX-M) -harboring E. coli isolates revealed that the bla(CTX-M-64) gene was integrated into the chromosome of ST10 E. coli B22 via ISEcp1-mediated transposition of a 9,467-bp sequence. The bla(CTX-M-123)-carrying Incl1 plasmid pB64 was 109,169 bp in length with pST108. The overall findings suggest that wastewater may act as a probable reservoir of clinically significant clonal lineages mediating antimicrobial resistance genes and chimeric genes that have not yet been identified from human isolates of domestic origin in Japan. |
資源タイプ(コンテンツの種類) |
|
|
内容記述 |
Article |
ISSN |
|
|
収録物識別子タイプ |
EISSN |
|
収録物識別子 |
1098-5336 |
書誌レコードID |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA00543249 |
PubMed |
|
|
|
関連識別子 |
https://pubmed.ncbi.nlm.nih.gov/31519655/ |
|
|
関連名称 |
31519655 |
権利 |
|
|
権利情報 |
© 2019 American Society for Microbiology. All Rights Reserved. |
出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |