Item type |
学術雑誌論文 / Journal Article(1) |
公開日 |
2023-01-19 |
タイトル |
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タイトル |
Development of a mouse iron overload-induced liver injury model and evaluation of the beneficial effects of placenta extract on iron metabolism |
言語 |
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言語 |
eng |
DOI |
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関連タイプ |
isIdenticalTo |
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関連識別子 |
https://doi.org/10.1016/j.heliyon.2019.e01637 |
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関連名称 |
10.1016/j.heliyon.2019.e01637 |
キーワード |
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主題 |
Molecular biology |
資源タイプ |
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資源 |
http://purl.org/coar/resource_type/c_6501 |
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タイプ |
journal article |
著者 |
Yamauchi, Akihiro
Kamiyoshi, Akiko
Sakurai, Takayuki
Miyazaki, Hiroyuki
Hirano, Eiichi
Lim, Hong Seok
Kaku, Taiichi
Shindo, Takayuki
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信州大学研究者総覧へのリンク |
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氏名 |
神吉, 昭子 |
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URL |
https://soar-rd.shinshu-u.ac.jp/profile/ja.OpLVuNkh.html |
信州大学研究者総覧へのリンク |
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氏名 |
桜井, 敬之 |
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URL |
https://soar-rd.shinshu-u.ac.jp/profile/ja.jaLUuNkh.html |
信州大学研究者総覧へのリンク |
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氏名 |
新藤, 隆行 |
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URL |
https://soar-rd.shinshu-u.ac.jp/profile/ja.OCLpOayV.html |
出版者 |
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出版者 |
Elsevier |
引用 |
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内容記述 |
Heliyon 5(5) : e01637-(2019) |
書誌情報 |
Heliyon
巻 5,
号 5,
p. e01637,
発行日 2019-05-10
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抄録 |
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内容記述 |
Hepatic iron deposition is seen in cases of chronic hepatitis and cirrhosis, and is a hallmark of a poorer prognosis. Iron deposition is also found in non-alcoholic steatohepatitis (NASH) patients. We have now developed a mouse model of NASH with hepatic iron deposition by combining a methione- and choline-deficient (MCD) diet with an iron-overload diet. Using this model, we evaluated the effects of human placenta extract (HPE), which has been shown to ameliorate the pathology of NASH. Four-week-old male C57BL/6 mice were fed the MCD diet with 2% iron for 12 weeks. In liver sections, iron deposition was first detected around the portal vein after 1 week. From there it spread throughout the parenchyma. Biliary iron concentrations were continuously elevated throughout the entire 12-week diet. As a compensatory response, the diet caused elevation of serum hepcidin, which accelerates excretion of iron from the body. Accumulation of F4/80-positive macrophages was detected within the sinusoids from the first week onward, and real-time PCR analysis revealed elevated hepatic expression of genes related inflammation and oxidative stress. In the model mice, HPE treatment led to a marked reduction of hepatic iron deposition with a corresponding increase in biliary iron excretion. Macrophage accumulation was much reduced by HPE treatment, as was the serum oxidation-reduction potential, an index of oxidative stress. These data indicate that by suppressing inflammation, oxidative stress and iron deposition, and enhancing iron excretion, HPE effectively ameliorates iron overload-induced liver injury. HPE administration may thus be an effective strategy for treating NASH. |
資源タイプ(コンテンツの種類) |
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内容記述 |
Article |
ISSN |
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収録物識別子タイプ |
EISSN |
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収録物識別子 |
2405-8440 |
PubMed |
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関連識別子 |
https://pubmed.ncbi.nlm.nih.gov/31193082/ |
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関連名称 |
31193082 |
権利 |
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権利情報 |
© 2019 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
出版タイプ |
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出版タイプ |
VoR |
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出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |