| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2023-02-07 |
| タイトル |
|
|
タイトル |
The accD3 gene for mycolic acid biosynthesis as a target for improving fatty acid production by fatty acid-producing Corynebacterium glutamicum strains |
| 言語 |
|
|
言語 |
eng |
| DOI |
|
|
関連タイプ |
isIdenticalTo |
|
|
関連識別子 |
https://doi.org/10.1007/s00253-018-9395-5 |
|
|
関連名称 |
10.1007/s00253-018-9395-5 |
| キーワード |
|
|
主題 |
Corynebacterium glutamicum, fatty acid production, accD3, mycolic acid, cell envelope permeability barrier |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 著者 |
Takeno, Seiki
Murata, Noriko
Kura, Moe
Takasaki, Manami
Hayashi, Mikiro
Ikeda, Masato
|
| 信州大学研究者総覧へのリンク |
|
|
表示名 |
竹野, 誠記 |
|
URL |
https://soar-rd.shinshu-u.ac.jp/profile/ja.HULePUkh.html |
| 信州大学研究者総覧へのリンク |
|
|
表示名 |
池田, 正人 |
|
URL |
https://soar-rd.shinshu-u.ac.jp/profile/ja.uhLNPUkh.html |
| 出版者 |
|
|
出版者 |
Springer International |
| 引用 |
|
|
内容記述タイプ |
Other |
|
内容記述 |
Applied microbiology and biotechnology. 102(24) : 10603-10612(2018) |
| 書誌情報 |
Applied microbiology and biotechnology.
巻 102,
号 24,
p. 10603-10612,
発行日 2018-10-01
|
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
We have recently developed Corynebacterium glutamicum strains that produce free fatty acids in culture supernatant due to enhanced fatty acid biosynthesis. Of these producing strains, the basic producer PAS-15 has a defect in the gene for a fatty acid biosynthesis repressor protein, and the advanced producer PCC-6 has two additional mutations to augment the production by strain PAS-15. The aim of the present study was to obtain novel genetic traits for improving fatty acid production by these producers. A new mutant with increased production derived from strain PAS-15 had a missense mutation in the accD3 gene (mutation accD3A433T), which is involved in the biosynthesis of mycolic acids that are cell envelope lipids of C. glutamicum, as the causal mutation. Mutation accD3A433T was verified to reduce the AccD3 enzymatic activity and increase fatty acid production in strain PAS-15 by 1.8-fold. Deletion of the accD3 gene in strain PAS-15, which was motivated by the characteristic of mutation accD3A433T, increased fatty acid production by 3.2-fold. Susceptibility of strain PAS-15 to vancomycin was significantly increased by accD3 gene deletion and by mutation accD3A433T to the intermediate level, suggesting that the cell envelope permeability barrier by mycolic acids is weakened by this engineering. Furthermore, mutation accD3A433T also increased fatty acid production in strain PCC-6 by 1.3-fold. These increased production levels were suggested to be involved not only in the redirection of carbon flux from mycolic acid biosynthesis to fatty acid production but also in the permeability of the cell envelope. |
| 資源タイプ（コンテンツの種類） |
|
|
内容記述タイプ |
Other |
|
内容記述 |
Article |
| ISSN |
|
|
収録物識別子タイプ |
EISSN |
|
収録物識別子 |
1432-0614 |
| PubMed |
|
|
|
識別子タイプ |
PMID |
|
|
関連識別子 |
https://pubmed.ncbi.nlm.nih.gov/30276713/ |
|
|
関連名称 |
30276713 |
| 権利 |
|
|
権利情報 |
This version of the article has been accepted for publication, after peer review (when applicable) and is subject to Springer Nature’s AM terms of use, but is not the Version of Record and does not reflect post-acceptance improvements, or any corrections. The Version of Record is available online at: https://doi.org/10.1007/s00253-018-9395-5 |
| 出版タイプ |
|
|
出版タイプ |
AM |
|
出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
ApplMicrobioBiotech102_10603.pdf
