{"_buckets": {"deposit": "45877ea5-de2a-4389-8fa6-1254c142523e"}, "_deposit": {"id": "2001447", "owners": [1], "pid": {"revision_id": 0, "type": "depid", "value": "2001447"}, "status": "published"}, "_oai": {"id": "oai:soar-ir.repo.nii.ac.jp:02001447", "sets": []}, "author_link": [], "control_number": "2001447", "item_1628147817048": {"attribute_name": "出版タイプ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_ab4af688f83e57aa", "subitem_version_type": "AM"}]}, "item_6_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2023-04-26", "bibliographicIssueDateType": "Issued"}, "bibliographicPageEnd": "8", "bibliographicPageStart": "1", "bibliographicVolumeNumber": "664", "bibliographic_titles": [{"bibliographic_title": "Biochemical and biophysical research communications", "bibliographic_titleLang": "en"}]}]}, "item_6_description_20": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "A myogenetic oligodeoxynucleotide, iSN04, is the 18-base single-stranded DNA that acts as an anti-nucleolin aptamer. iSN04 has been reported to restore myogenic differentiation by suppressing inflammatory responses in myoblasts isolated from patients with diabetes or healthy myoblasts exposed to cancer-releasing factors. Thus, iSN04 is expected to be a nucleic acid drug for the muscle wasting associated with chronic diseases. The present study investigated the anti-inflammatory mechanism of iSN04 in the murine myoblast cell line C2C12. Tumor necrosis factor-α (TNF-α) or Toll-like receptor (TLR) ligands (Pam3CSK4 and FSL-1) induced nuclear translocation and transcriptional activity of nuclear factor-κB (NF-κB), resulting in upregulated expression of TNF-α and interleukin-6. Pre-treatment with iSN04 significantly suppressed these inflammatory responses by inhibiting the nuclear accumulation of β-catenin induced by TNF-α or TLR ligands. These results demonstrate that antagonizing nucleolin with iSN04 downregulates the inflammatory effect mediated by the β-catenin/NF-κB signaling pathway in C2C12 cells. In addition, the anti-inflammatory effects of iSN04 were also observed in the rat smooth muscle cell line A10 and the murine adipocyte-like fibroblast cell line 3T3-L1, suggesting that iSN04 may be useful in preventing inflammation induced by metabolic disorders.", "subitem_description_type": "Abstract"}]}, "item_6_description_30": {"attribute_name": "資源タイプ（コンテンツの種類）", "attribute_value_mlt": [{"subitem_description": "Article", "subitem_description_type": "Other"}]}, "item_6_description_5": {"attribute_name": "引用", "attribute_value_mlt": [{"subitem_description": "Biochemical and biophysical research communications 664 : 1-8, (2023)", "subitem_description_type": "Other"}]}, "item_6_link_3": {"attribute_name": "信州大学研究者総覧へのリンク", "attribute_value_mlt": [{"subitem_link_text": "三谷, 塁一", "subitem_link_url": "https://soar-rd.shinshu-u.ac.jp/profile/ja.ypcCPUkh.html"}, {"subitem_link_text": "高谷, 智英", "subitem_link_url": "https://soar-rd.shinshu-u.ac.jp/profile/ja.yUcePUkh.html"}]}, "item_6_publisher_4": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "Elsevier"}]}, "item_6_relation_47": {"attribute_name": "PubMed", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "37127012"}], "subitem_relation_type_id": {"subitem_relation_type_id_text": "https://pubmed.ncbi.nlm.nih.gov/37127012/", "subitem_relation_type_select": "PMID"}}]}, "item_6_relation_48": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "10.1016/j.bbrc.2023.04.098"}], "subitem_relation_type": "isVersionOf", "subitem_relation_type_id": {"subitem_relation_type_id_text": "https://doi.org/10.1016/j.bbrc.2023.04.098", "subitem_relation_type_select": "DOI"}}]}, "item_6_source_id_35": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "1090-2104", "subitem_source_identifier_type": "EISSN"}]}, "item_6_source_id_39": {"attribute_name": "NII ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "1090-2104", "subitem_source_identifier_type": "EISSN"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Yamamoto, Machi", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Miyoshi, Mana", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Morioka, Kamino", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Mitani, Takakazu", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Takaya, Tomohide", "creatorNameLang": "en"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2024-04-26"}], "download_preview_message": "Download / Preview is available from 2024/4/25.", "file_order": 0, "filename": "BBRC664_1.pdf", "format": "application/pdf", "future_date_message": "Download is available from 2024/4/25.", "is_thumbnail": false, "licensetype": "license_5", "mimetype": "application/pdf", "size": 0, "url": {"url": "https://soar-ir.repo.nii.ac.jp/record/2001447/files/BBRC664_1.pdf"}, "version_id": "b1aee7fb-356b-490c-9604-decc9e2ee6a6"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "β-catenin", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Inflammatory response", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Myoblast", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Myogenetic oligodeoxynucleotide (myoDN)", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Nuclear factor-kB (NF-kB)", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Nucleolin", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Anti-nucleolin aptamer, iSN04, inhibits the inflammatory responses in C2C12 myoblasts by modulating the β-catenin/NF-κB signaling pathway", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Anti-nucleolin aptamer, iSN04, inhibits the inflammatory responses in C2C12 myoblasts by modulating the β-catenin/NF-κB signaling pathway", "subitem_title_language": "en"}]}, "item_type_id": "6", "owner": "1", "path": ["1017"], "permalink_uri": "http://hdl.handle.net/10091/0002001447", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2023-05-08"}, "publish_date": "2023-05-08", "publish_status": "0", "recid": "2001447", "relation": {}, "relation_version_is_last": true, "title": ["Anti-nucleolin aptamer, iSN04, inhibits the inflammatory responses in C2C12 myoblasts by modulating the β-catenin/NF-κB signaling pathway"], "weko_shared_id": -1}