WEKO3
アイテム
{"_buckets": {"deposit": "844ae914-d60b-40c1-aff4-82bdc0ef5e9a"}, "_deposit": {"id": "20696", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "20696"}, "status": "published"}, "_oai": {"id": "oai:soar-ir.repo.nii.ac.jp:00020696", "sets": ["1017"]}, "author_link": ["107972", "107973", "107974", "107975", "107976"], "item_1628147817048": {"attribute_name": "出版タイプ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_ab4af688f83e57aa", "subitem_version_type": "AM"}]}, "item_6_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2017-11", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "11", "bibliographicPageEnd": "1885", "bibliographicPageStart": "1880", "bibliographicVolumeNumber": "88", "bibliographic_titles": [{"bibliographic_title": "ANIMAL SCIENCE JOURNAL"}]}]}, "item_6_description_20": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "Cell-cell fusion has been a great technology to generate valuable hybrid cells and organisms such as hybridomas. In this study, skeletal muscle myoblasts were utilized to establish a novel method for autonomous xenogenic cell fusion. Myoblasts are mononuclear myogenic precursor cells and fuse mutually to form multinuclear myotubes. We generated murine myoblasts (mMBs) expressing green fluorescent protein (GFP) termed mMB-GFP, and the chick myoblasts (chMBs) expressing Discosoma red fluorescent protein (DsRed) termed chMB-DsRed. mMB-GFP and chMB-DsRed were cocultured and induced to differentiate. After 24h, the multinuclear myotubes expressing both GFP and DsRed were observed, indicating that mMBs and chMBs interspecifically fuse. These GFP(+)/DsRed(+) hybrid myotubes were able to survive and grew to hyper-multinucleated mature form. We also found that undifferentiated mMB-GFP efficiently fuse to the chMB-DsRed-derived myotubes. This is the first evidence for the autonomous xenogenic fusion of mammalian and avian cells. Myoblast-based fusogenic technique will open up an alternative direction to create novel hybrid products.", "subitem_description_type": "Abstract"}]}, "item_6_description_30": {"attribute_name": "資源タイプ(コンテンツの種類)", "attribute_value_mlt": [{"subitem_description": "Article", "subitem_description_type": "Other"}]}, "item_6_description_5": {"attribute_name": "引用", "attribute_value_mlt": [{"subitem_description": "ANIMAL SCIENCE JOURNAL. 88(11):1880-1885 (2017)", "subitem_description_type": "Other"}]}, "item_6_link_3": {"attribute_name": "信州大学研究者総覧へのリンク", "attribute_value_mlt": [{"subitem_link_text": "Takaya, Tomohide", "subitem_link_url": "http://soar-rd.shinshu-u.ac.jp/profile/ja.yUcePUkh.html"}, {"subitem_link_text": "Ono, Tamao", "subitem_link_url": "http://soar-rd.shinshu-u.ac.jp/profile/ja.WFfCPpkh.html"}, {"subitem_link_text": "Kagami, Hiroshi", "subitem_link_url": "http://soar-rd.shinshu-u.ac.jp/profile/ja.OeyUPUkh.html"}]}, "item_6_link_67": {"attribute_name": "WoS", "attribute_value_mlt": [{"subitem_link_text": "Web of Science", "subitem_link_url": "http://gateway.isiknowledge.com/gateway/Gateway.cgi?\u0026GWVersion=2\u0026SrcAuth=ShinshuUniv\u0026SrcApp=ShinshuUniv\u0026DestLinkType=FullRecord\u0026DestApp=WOS\u0026KeyUT=000414462000028"}]}, "item_6_publisher_4": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "WILEY"}]}, "item_6_relation_47": {"attribute_name": "PubMed", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "28782148"}], "subitem_relation_type_id": {"subitem_relation_type_id_text": "https://pubmed.ncbi.nlm.nih.gov/28782148/", "subitem_relation_type_select": "PMID"}}]}, "item_6_relation_48": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "10.1111/asj.12884"}], "subitem_relation_type_id": {"subitem_relation_type_id_text": "https://doi.org/10.1111/asj.12884", "subitem_relation_type_select": "DOI"}}]}, "item_6_rights_62": {"attribute_name": "権利", "attribute_value_mlt": [{"subitem_rights": "© 2017 Japanese Society of Animal Science, This is the peer reviewed version of the following article: Anim Sci J, 88: 1880-1885, 2017, which has been published in final form at https://doi.org/10.1111/asj.12884 . This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.\u003cbr/\u003e"}]}, "item_6_select_64": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_select_item": "author"}]}, "item_6_source_id_35": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "1344-3941", "subitem_source_identifier_type": "ISSN"}]}, "item_6_source_id_39": {"attribute_name": "NII ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "1344-3941", "subitem_source_identifier_type": "ISSN"}]}, "item_6_source_id_40": {"attribute_name": "書誌レコードID", "attribute_value_mlt": [{"subitem_source_identifier": "AA11677214", "subitem_source_identifier_type": "NCID"}]}, "item_6_text_69": {"attribute_name": "wosonly authkey", "attribute_value_mlt": [{"subitem_text_value": "MYOMAKER; PROTOPLASTS; MYOCYTES; HYBRIDS; MYOD"}]}, "item_6_text_70": {"attribute_name": "wosonly keywords", "attribute_value_mlt": [{"subitem_text_value": "cell fusion; hybrid cell; myotube formation; skeletal muscle myoblast"}]}, "item_6_textarea_68": {"attribute_name": "wosonly abstract", "attribute_value_mlt": [{"subitem_textarea_value": "Cell-cell fusion has been a great technology to generate valuable hybrid cells and organisms such as hybridomas. In this study, skeletal muscle myoblasts were utilized to establish a novel method for autonomous xenogenic cell fusion. Myoblasts are mononuclear myogenic precursor cells and fuse mutually to form multinuclear myotubes. We generated murine myoblasts (mMBs) expressing green fluorescent protein (GFP) termed mMB-GFP, and the chick myoblasts (chMBs) expressing Discosoma red fluorescent protein (DsRed) termed chMB-DsRed. mMB-GFP and chMB-DsRed were cocultured and induced to differentiate. After 24h, the multinuclear myotubes expressing both GFP and DsRed were observed, indicating that mMBs and chMBs interspecifically fuse. These GFP(+)/DsRed(+) hybrid myotubes were able to survive and grew to hyper-multinucleated mature form. We also found that undifferentiated mMB-GFP efficiently fuse to the chMB-DsRed-derived myotubes. This is the first evidence for the autonomous xenogenic fusion of mammalian and avian cells. Myoblast-based fusogenic technique will open up an alternative direction to create novel hybrid products."}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Takaya, Tomohide"}], "nameIdentifiers": [{"nameIdentifier": "107972", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Nihashi, Yuma"}], "nameIdentifiers": [{"nameIdentifier": "107973", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kojima, Shotaro"}], "nameIdentifiers": [{"nameIdentifier": "107974", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Ono, Tamao"}], "nameIdentifiers": [{"nameIdentifier": "107975", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kagami, Hiroshi"}], "nameIdentifiers": [{"nameIdentifier": "107976", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2019-05-08"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "2017_AnimSciJ_Takaya_SOAR.pdf", "filesize": [{"value": "1.3 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensefree": "© 2017 Japanese Society of Animal Science, This is the peer reviewed version of the following article: Anim Sci J, 88: 1880-1885, 2017, which has been published in final form at https://doi.org/10.1111/asj.12884 . This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.\u003cbr/\u003e", "licensetype": "license_note", "mimetype": "application/pdf", "size": 1300000.0, "url": {"label": "2017_AnimSciJ_Takaya_SOAR.pdf", "url": "https://soar-ir.repo.nii.ac.jp/record/20696/files/2017_AnimSciJ_Takaya_SOAR.pdf"}, "version_id": "548e42fb-cdd4-4dec-995f-d402dee248fd"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "cell fusion", "subitem_subject_scheme": "Other"}, {"subitem_subject": "hybrid cell", "subitem_subject_scheme": "Other"}, {"subitem_subject": "myotube formation", "subitem_subject_scheme": "Other"}, {"subitem_subject": "skeletal muscle myoblast", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Autonomous xenogenic cell fusion of murine and chick skeletal muscle myoblasts", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Autonomous xenogenic cell fusion of murine and chick skeletal muscle myoblasts", "subitem_title_language": "en"}]}, "item_type_id": "6", "owner": "1", "path": ["1017"], "permalink_uri": "http://hdl.handle.net/10091/00021454", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2019-05-08"}, "publish_date": "2019-05-08", "publish_status": "0", "recid": "20696", "relation": {}, "relation_version_is_last": true, "title": ["Autonomous xenogenic cell fusion of murine and chick skeletal muscle myoblasts"], "weko_shared_id": -1}
Autonomous xenogenic cell fusion of murine and chick skeletal muscle myoblasts
http://hdl.handle.net/10091/00021454
http://hdl.handle.net/10091/0002145416c92e75-a9bd-47c8-bcc3-89f5d2f3f393
名前 / ファイル | ライセンス | アクション |
---|---|---|
![]() |
|
Item type | 学術雑誌論文 / Journal Article(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2019-05-08 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Autonomous xenogenic cell fusion of murine and chick skeletal muscle myoblasts | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | cell fusion | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | hybrid cell | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | myotube formation | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | skeletal muscle myoblast | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | journal article | |||||
著者 |
Takaya, Tomohide
× Takaya, Tomohide× Nihashi, Yuma× Kojima, Shotaro× Ono, Tamao× Kagami, Hiroshi |
|||||
信州大学研究者総覧へのリンク | ||||||
氏名 | Takaya, Tomohide | |||||
URL | http://soar-rd.shinshu-u.ac.jp/profile/ja.yUcePUkh.html | |||||
信州大学研究者総覧へのリンク | ||||||
氏名 | Ono, Tamao | |||||
URL | http://soar-rd.shinshu-u.ac.jp/profile/ja.WFfCPpkh.html | |||||
信州大学研究者総覧へのリンク | ||||||
氏名 | Kagami, Hiroshi | |||||
URL | http://soar-rd.shinshu-u.ac.jp/profile/ja.OeyUPUkh.html | |||||
出版者 | ||||||
出版者 | WILEY | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | ANIMAL SCIENCE JOURNAL. 88(11):1880-1885 (2017) | |||||
書誌情報 |
ANIMAL SCIENCE JOURNAL 巻 88, 号 11, p. 1880-1885, 発行日 2017-11 |
|||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Cell-cell fusion has been a great technology to generate valuable hybrid cells and organisms such as hybridomas. In this study, skeletal muscle myoblasts were utilized to establish a novel method for autonomous xenogenic cell fusion. Myoblasts are mononuclear myogenic precursor cells and fuse mutually to form multinuclear myotubes. We generated murine myoblasts (mMBs) expressing green fluorescent protein (GFP) termed mMB-GFP, and the chick myoblasts (chMBs) expressing Discosoma red fluorescent protein (DsRed) termed chMB-DsRed. mMB-GFP and chMB-DsRed were cocultured and induced to differentiate. After 24h, the multinuclear myotubes expressing both GFP and DsRed were observed, indicating that mMBs and chMBs interspecifically fuse. These GFP(+)/DsRed(+) hybrid myotubes were able to survive and grew to hyper-multinucleated mature form. We also found that undifferentiated mMB-GFP efficiently fuse to the chMB-DsRed-derived myotubes. This is the first evidence for the autonomous xenogenic fusion of mammalian and avian cells. Myoblast-based fusogenic technique will open up an alternative direction to create novel hybrid products. | |||||
資源タイプ(コンテンツの種類) | ||||||
内容記述タイプ | Other | |||||
内容記述 | Article | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1344-3941 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA11677214 | |||||
PubMed | ||||||
識別子タイプ | PMID | |||||
関連識別子 | https://pubmed.ncbi.nlm.nih.gov/28782148/ | |||||
関連名称 | 28782148 | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | https://doi.org/10.1111/asj.12884 | |||||
関連名称 | 10.1111/asj.12884 | |||||
権利 | ||||||
権利情報 | © 2017 Japanese Society of Animal Science, This is the peer reviewed version of the following article: Anim Sci J, 88: 1880-1885, 2017, which has been published in final form at https://doi.org/10.1111/asj.12884 . This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.<br/> | |||||
出版タイプ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
WoS | ||||||
表示名 | Web of Science | |||||
URL | http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=ShinshuUniv&SrcApp=ShinshuUniv&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000414462000028 |