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  1. 070 農学部, 大学院農学研究科
  2. 0701 学術論文

Autonomous xenogenic cell fusion of murine and chick skeletal muscle myoblasts

http://hdl.handle.net/10091/00021454
http://hdl.handle.net/10091/00021454
16c92e75-a9bd-47c8-bcc3-89f5d2f3f393
名前 / ファイル ライセンス アクション
2017_AnimSciJ_Takaya_SOAR.pdf 2017_AnimSciJ_Takaya_SOAR.pdf (1.3 MB)
Item type 学術雑誌論文 / Journal Article(1)
公開日 2019-05-08
タイトル
言語 en
タイトル Autonomous xenogenic cell fusion of murine and chick skeletal muscle myoblasts
言語
言語 eng
キーワード
主題Scheme Other
主題 cell fusion
キーワード
主題Scheme Other
主題 hybrid cell
キーワード
主題Scheme Other
主題 myotube formation
キーワード
主題Scheme Other
主題 skeletal muscle myoblast
資源タイプ
資源 http://purl.org/coar/resource_type/c_6501
タイプ journal article
著者 Takaya, Tomohide

× Takaya, Tomohide

WEKO 107972

Takaya, Tomohide

Search repository
Nihashi, Yuma

× Nihashi, Yuma

WEKO 107973

Nihashi, Yuma

Search repository
Kojima, Shotaro

× Kojima, Shotaro

WEKO 107974

Kojima, Shotaro

Search repository
Ono, Tamao

× Ono, Tamao

WEKO 107975

Ono, Tamao

Search repository
Kagami, Hiroshi

× Kagami, Hiroshi

WEKO 107976

Kagami, Hiroshi

Search repository
信州大学研究者総覧へのリンク
氏名 Takaya, Tomohide
URL http://soar-rd.shinshu-u.ac.jp/profile/ja.yUcePUkh.html
信州大学研究者総覧へのリンク
氏名 Ono, Tamao
URL http://soar-rd.shinshu-u.ac.jp/profile/ja.WFfCPpkh.html
信州大学研究者総覧へのリンク
氏名 Kagami, Hiroshi
URL http://soar-rd.shinshu-u.ac.jp/profile/ja.OeyUPUkh.html
出版者
出版者 WILEY
引用
内容記述タイプ Other
内容記述 ANIMAL SCIENCE JOURNAL. 88(11):1880-1885 (2017)
書誌情報 ANIMAL SCIENCE JOURNAL

巻 88, 号 11, p. 1880-1885, 発行日 2017-11
抄録
内容記述タイプ Abstract
内容記述 Cell-cell fusion has been a great technology to generate valuable hybrid cells and organisms such as hybridomas. In this study, skeletal muscle myoblasts were utilized to establish a novel method for autonomous xenogenic cell fusion. Myoblasts are mononuclear myogenic precursor cells and fuse mutually to form multinuclear myotubes. We generated murine myoblasts (mMBs) expressing green fluorescent protein (GFP) termed mMB-GFP, and the chick myoblasts (chMBs) expressing Discosoma red fluorescent protein (DsRed) termed chMB-DsRed. mMB-GFP and chMB-DsRed were cocultured and induced to differentiate. After 24h, the multinuclear myotubes expressing both GFP and DsRed were observed, indicating that mMBs and chMBs interspecifically fuse. These GFP(+)/DsRed(+) hybrid myotubes were able to survive and grew to hyper-multinucleated mature form. We also found that undifferentiated mMB-GFP efficiently fuse to the chMB-DsRed-derived myotubes. This is the first evidence for the autonomous xenogenic fusion of mammalian and avian cells. Myoblast-based fusogenic technique will open up an alternative direction to create novel hybrid products.
資源タイプ(コンテンツの種類)
内容記述タイプ Other
内容記述 Article
ISSN
収録物識別子タイプ ISSN
収録物識別子 1344-3941
書誌レコードID
収録物識別子タイプ NCID
収録物識別子 AA11677214
PubMed
識別子タイプ PMID
関連識別子 https://pubmed.ncbi.nlm.nih.gov/28782148/
関連名称 28782148
DOI
識別子タイプ DOI
関連識別子 https://doi.org/10.1111/asj.12884
関連名称 10.1111/asj.12884
権利
権利情報 © 2017 Japanese Society of Animal Science, This is the peer reviewed version of the following article: Anim Sci J, 88: 1880-1885, 2017, which has been published in final form at https://doi.org/10.1111/asj.12884 . This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.<br/>
出版タイプ
出版タイプ AM
出版タイプResource http://purl.org/coar/version/c_ab4af688f83e57aa
WoS
表示名 Web of Science
URL http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=ShinshuUniv&SrcApp=ShinshuUniv&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000414462000028
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