| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2020-02-28 |
| タイトル |
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|
タイトル |
Targeted mutagenesis in chicken using CRISPR/Cas9 system |
| 言語 |
|
|
言語 |
eng |
| DOI |
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関連識別子 |
https://doi.org/10.1038/srep23980 |
|
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関連名称 |
10.1038/srep23980 |
| キーワード |
|
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主題 |
Agricultural genetics, Genetic engineering |
| 資源タイプ |
|
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資源 |
http://purl.org/coar/resource_type/c_6501 |
|
タイプ |
journal article |
| 著者 |
Oishi, Isao
Yoshii, Kyoko
Miyahara, Daichi
Kagami, Hiroshi
Tagami, Takahiro
|
| 信州大学研究者総覧へのリンク |
|
|
氏名 |
Miyahara, Daichi |
|
URL |
http://soar-rd.shinshu-u.ac.jp/profile/ja.uhfmbCkF.html |
| 信州大学研究者総覧へのリンク |
|
|
氏名 |
Kagami, Hiroshi |
|
URL |
http://soar-rd.shinshu-u.ac.jp/profile/ja.OeyUPUkh.html |
| 出版者 |
|
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出版者 |
NATURE PUBLISHING GROUP |
| 引用 |
|
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内容記述 |
SCIENTIFIC REPORTS.6:23980(2016) |
| 書誌情報 |
SCIENTIFIC REPORTS
巻 6,
p. 23980,
発行日 2016-04-06
|
| 抄録 |
|
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内容記述 |
The CRISPR/Cas9 system is a simple and powerful tool for genome editing in various organisms including livestock animals. However, the system has not been applied to poultry because of the difficulty in accessing their zygotes. Here we report the implementation of CRISPR/Cas9-mediated gene targeting in chickens. Two egg white genes, ovalbumin and ovomucoid, were efficiently (> 90%) mutagenized in cultured chicken primordial germ cells (PGCs) by transfection of circular plasmids encoding Cas9, a single guide RNA, and a gene encoding drug resistance, followed by transient antibiotic selection. We transplanted CRISPR-induced mutant-ovomucoid PGCs into recipient chicken embryos and established three germline chimeric roosters (G0). All of the roosters had donor-derived mutant-ovomucoid spermatozoa, and the two with a high transmission rate of donor-derived gametes produced heterozygous mutant ovomucoid chickens as about half of their donor-derived offspring in the next generation (G1). Furthermore, we generated ovomucoid homozygous mutant offspring (G2) by crossing the G1 mutant chickens. Taken together, these results demonstrate that the CRISPR/Cas9 system is a simple and effective gene-targeting method in chickens. |
| 資源タイプ（コンテンツの種類） |
|
| ISSN |
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収録物識別子タイプ |
ISSN |
|
収録物識別子 |
2045-2322 |
| PubMed |
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識別子タイプ |
PMID |
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関連識別子 |
https://pubmed.ncbi.nlm.nih.gov/27050479/ |
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関連名称 |
27050479 |
| 権利 |
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権利情報 |
This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
| 出版タイプ |
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|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| WoS |
|
|
URL |
http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=ShinshuUniv&SrcApp=ShinshuUniv&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000373432200001 |
26450376_03.pdf (6.8 MB)
