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  1. 070 農学部, 大学院農学研究科
  2. 0701 学術論文

Targeted mutagenesis in chicken using CRISPR/Cas9 system

http://hdl.handle.net/10091/00021880
http://hdl.handle.net/10091/00021880
fc376fdc-d48c-4f2d-95f1-1bb8a8d9aefa
名前 / ファイル ライセンス アクション
26450376_03.pdf 26450376_03.pdf (6.8 MB)
Item type 学術雑誌論文 / Journal Article(1)
公開日 2020-02-28
タイトル
タイトル Targeted mutagenesis in chicken using CRISPR/Cas9 system
言語
言語 eng
DOI
関連識別子 https://doi.org/10.1038/srep23980
関連名称 10.1038/srep23980
キーワード
主題 Agricultural genetics, Genetic engineering
資源タイプ
資源 http://purl.org/coar/resource_type/c_6501
タイプ journal article
著者 Oishi, Isao

× Oishi, Isao

Oishi, Isao

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Yoshii, Kyoko

× Yoshii, Kyoko

Yoshii, Kyoko

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Miyahara, Daichi

× Miyahara, Daichi

Miyahara, Daichi

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Kagami, Hiroshi

× Kagami, Hiroshi

Kagami, Hiroshi

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Tagami, Takahiro

× Tagami, Takahiro

Tagami, Takahiro

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信州大学研究者総覧へのリンク
氏名 Miyahara, Daichi
URL http://soar-rd.shinshu-u.ac.jp/profile/ja.uhfmbCkF.html
信州大学研究者総覧へのリンク
氏名 Kagami, Hiroshi
URL http://soar-rd.shinshu-u.ac.jp/profile/ja.OeyUPUkh.html
出版者
出版者 NATURE PUBLISHING GROUP
引用
内容記述 SCIENTIFIC REPORTS.6:23980(2016)
書誌情報 SCIENTIFIC REPORTS

巻 6, p. 23980, 発行日 2016-04-06
抄録
内容記述 The CRISPR/Cas9 system is a simple and powerful tool for genome editing in various organisms including livestock animals. However, the system has not been applied to poultry because of the difficulty in accessing their zygotes. Here we report the implementation of CRISPR/Cas9-mediated gene targeting in chickens. Two egg white genes, ovalbumin and ovomucoid, were efficiently (> 90%) mutagenized in cultured chicken primordial germ cells (PGCs) by transfection of circular plasmids encoding Cas9, a single guide RNA, and a gene encoding drug resistance, followed by transient antibiotic selection. We transplanted CRISPR-induced mutant-ovomucoid PGCs into recipient chicken embryos and established three germline chimeric roosters (G0). All of the roosters had donor-derived mutant-ovomucoid spermatozoa, and the two with a high transmission rate of donor-derived gametes produced heterozygous mutant ovomucoid chickens as about half of their donor-derived offspring in the next generation (G1). Furthermore, we generated ovomucoid homozygous mutant offspring (G2) by crossing the G1 mutant chickens. Taken together, these results demonstrate that the CRISPR/Cas9 system is a simple and effective gene-targeting method in chickens.
資源タイプ(コンテンツの種類)
ISSN
収録物識別子タイプ ISSN
収録物識別子 2045-2322
PubMed
識別子タイプ PMID
関連識別子 https://pubmed.ncbi.nlm.nih.gov/27050479/
関連名称 27050479
権利
権利情報 This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
出版タイプ
出版タイプ VoR
出版タイプResource http://purl.org/coar/version/c_970fb48d4fbd8a85
WoS
URL http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=ShinshuUniv&SrcApp=ShinshuUniv&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000373432200001
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