{"_buckets": {"deposit": "157bf650-cfa5-4e7f-af53-5b1f08e95c62"}, "_deposit": {"id": "3671", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "3671"}, "status": "published"}, "_oai": {"id": "oai:soar-ir.repo.nii.ac.jp:00003671"}, "item_6_biblio_info_6": {"attribute_name": "\u66f8\u8a8c\u60c5\u5831", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2015", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "3", "bibliographicPageEnd": "614", "bibliographicPageStart": "606", "bibliographicVolumeNumber": "29", "bibliographic_titles": [{"bibliographic_title": "LEUKEMIA"}]}]}, "item_6_description_19": {"attribute_name": "\u5185\u5bb9\u8a18\u8ff0", "attribute_value_mlt": [{"subitem_description": "advance online publication, August 8, 2014 [Epub ahead of print]", "subitem_description_type": "Other"}]}, "item_6_description_30": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7\uff08\u30b3\u30f3\u30c6\u30f3\u30c4\u306e\u7a2e\u985e\uff09", "attribute_value_mlt": [{"subitem_description": "Article", "subitem_description_type": "Other"}]}, "item_6_description_5": {"attribute_name": "\u5f15\u7528", "attribute_value_mlt": [{"subitem_description": "LEUKEMIA. 29(3):606-614 (2015)\n", "subitem_description_type": "Other"}]}, "item_6_link_3": {"attribute_name": "\u4fe1\u5dde\u5927\u5b66\u7814\u7a76\u8005\u7dcf\u89a7\u3078\u306e\u30ea\u30f3\u30af", "attribute_value_mlt": [{"subitem_link_text": "Nakazawa, Yozo", "subitem_link_url": "http://soar-rd.shinshu-u.ac.jp/profile/ja.yCSFuNkh.html"}]}, "item_6_link_67": {"attribute_name": "WoS", "attribute_value_mlt": [{"subitem_link_text": "Web of Science", "subitem_link_url": "http://gateway.isiknowledge.com/gateway/Gateway.cgi?\u0026GWVersion=2\u0026SrcAuth=ShinshuUniv\u0026SrcApp=ShinshuUniv\u0026DestLinkType=FullRecord\u0026DestApp=WOS\u0026KeyUT=000350734300010"}]}, "item_6_publisher_4": {"attribute_name": "\u51fa\u7248\u8005", "attribute_value_mlt": [{"subitem_publisher": "NATURE PUBLISHING GROUP"}]}, "item_6_relation_47": {"attribute_name": "PubMed", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "25102944"}], "subitem_relation_type_id": {"subitem_relation_type_id_text": "http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?CMD=search\u0026DB=pubmed\u0026term=25102944", "subitem_relation_type_select": "PMID"}}]}, "item_6_relation_48": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "10.1038/leu.2014.239"}], "subitem_relation_type_id": {"subitem_relation_type_id_text": "http://dx.doi.org/10.1038/leu.2014.239", "subitem_relation_type_select": "DOI"}}]}, "item_6_select_64": {"attribute_name": "\u8457\u8005\u7248\u30d5\u30e9\u30b0", "attribute_value_mlt": [{"subitem_select_item": "author"}]}, "item_6_source_id_35": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "0887-6924", "subitem_source_identifier_type": "ISSN"}]}, "item_6_source_id_39": {"attribute_name": "NII ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "0887-6924", "subitem_source_identifier_type": "ISSN"}]}, "item_6_text_70": {"attribute_name": "wosonly keywords", "attribute_value_mlt": [{"subitem_text_value": "CHRONIC MYELOGENOUS LEUKEMIA; STEM-CELLS; RAS MUTATIONS; SCID MICE; THROMBOPOIETIN; REPOPULATION; PATHOGENESIS; IMPROVEMENT; MOUSE; MODEL"}]}, "item_6_textarea_68": {"attribute_name": "wosonly abstract", "attribute_value_mlt": [{"subitem_textarea_value": "Using serum-containing culture, we examined whether AGM-S3 stromal cells, alone or in combination with hematopoietic growth factor(s), stimulated the proliferation of CD34(+) cells from patients with juvenile myelomonocytic leukemia (JMML). AGM-S3 cells in concert with stem cell factor plus thrombopoietin increased the numbers of peripheral blood CD34(+) cells to approximately 20-fold of the input value after 2 weeks in nine JMML patients with either PTPN11 mutations or RAS mutations, who received allogeneic hematopoietic transplantation. Granulocyte-macrophage colony-stimulating factor (GM-CSF) also augmented the proliferation of JMML CD34(+) cells on AGM-S3 cells. The expansion potential of CD34(+) cells was markedly low in four patients who achieved spontaneous hematological improvement. A large proportion of day-14-cultured CD34(+) cells were negative for CD38 and cryopreservable. Cultured JMML CD34(+)CD38(-) cells expressed CD117, CD116, c-mpl, CD123, CD90, but not CXCR4, and formed GM and erythroid colonies. Day-7-cultured CD34(+) cells from two of three JMML patients injected intrafemorally into immunodeficient mice stimulated with human GM-CSF after transplantation displayed significant hematopoietic reconstitution. The abilities of OP9 cells and MS-5 cells were one-third and one-tenth, respectively, of the value obtained with AGM-S3 cells. Our culture system may provide a useful tool for elucidating leukemogenesis and for therapeutic approaches in JMML."}]}, "item_creator": {"attribute_name": "\u8457\u8005", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Sakashita,  Kazuo"}], "nameIdentifiers": [{"nameIdentifier": "6195", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kato,  Itaru"}], "nameIdentifiers": [{"nameIdentifier": "6196", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Daifu,  Tomoo"}], "nameIdentifiers": [{"nameIdentifier": "6197", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Saida,  Satoshi"}], "nameIdentifiers": [{"nameIdentifier": "6198", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Hiramatsu,  Hidefumi"}], "nameIdentifiers": [{"nameIdentifier": "6199", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Nishinaka,  Yoko"}], "nameIdentifiers": [{"nameIdentifier": "6200", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Ebihara,  Yasuhiro"}], "nameIdentifiers": [{"nameIdentifier": "6201", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Ma,  Feng"}], "nameIdentifiers": [{"nameIdentifier": "6202", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Matsuda,  Kazuyuki"}], "nameIdentifiers": [{"nameIdentifier": "6203", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Saito,  Shoji"}], "nameIdentifiers": [{"nameIdentifier": "6204", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Hirabayashi,  Koichi"}], "nameIdentifiers": [{"nameIdentifier": "6205", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kurata,  Takashi"}], "nameIdentifiers": [{"nameIdentifier": "6206", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Uyen,  Le Thanh Nha"}], "nameIdentifiers": [{"nameIdentifier": "6207", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Nakazawa,  Yozo"}], "nameIdentifiers": [{"nameIdentifier": "6208", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Tsuji,  Kohichiro"}], "nameIdentifiers": [{"nameIdentifier": "6209", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Heike,  Toshio"}], "nameIdentifiers": [{"nameIdentifier": "6210", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Nakahata,  Tatsutoshi"}], "nameIdentifiers": [{"nameIdentifier": "6211", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Koike,  Kenichi"}], "nameIdentifiers": [{"nameIdentifier": "6212", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "\u30d5\u30a1\u30a4\u30eb\u60c5\u5831", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2015-09-24"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "In_vitro_expansion_of_CD34+CD38-_cells.pdf", "filesize": [{"value": "1.2 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 1200000.0, "url": {"label": "In_vitro_expansion_of_CD34+CD38-_cells.pdf", "url": "https://soar-ir.repo.nii.ac.jp/record/3671/files/In_vitro_expansion_of_CD34+CD38-_cells.pdf"}, "version_id": "6d208ec8-6a3e-448e-82fb-21240c7b7389"}]}, "item_language": {"attribute_name": "\u8a00\u8a9e", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "In vitro expansion of CD34+CD38- cells under stimulation with hematopoietic growth factors on AGM-S3 cells in juvenile myelomonocytic leukemia", "item_titles": {"attribute_name": "\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_title": "In vitro expansion of CD34+CD38- cells under stimulation with hematopoietic growth factors on AGM-S3 cells in juvenile myelomonocytic leukemia"}]}, "item_type_id": "6", "owner": "1", "path": ["461/462"], "permalink_uri": "http://hdl.handle.net/10091/18010", "pubdate": {"attribute_name": "\u516c\u958b\u65e5", "attribute_name_i18n": "\u516c\u958b\u65e5", "attribute_value": "2015-01-06"}, "publish_date": "2015-01-06", "publish_status": "0", "recid": "3671", "relation": {}, "relation_version_is_last": true, "title": ["In vitro expansion of CD34+CD38- cells under stimulation with hematopoietic growth factors on AGM-S3 cells in juvenile myelomonocytic leukemia"], "weko_shared_id": 3}