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In contrast to AA amyloidosis-susceptible mouse strains that concomitantly express serum amyloid A precursor protein (SAA) types 1 and 2 isoforms encoded by the Saa1 and Saa2 genes, respectively, in response to inflammatory stimulation from the liver, CE/J mice express only a single SAA isoform named SAA2.2. In addition, CE/J mice uniquely possess a Q30L amino acid substitution in SAA2.2 that inhibits amyloidogenesis. To elucidate the genetic basis underlying the expression of only a single SAA isoform in this strain, we conducted PCR cloning and nucleotide sequencing of the Saa1 and Saa2 genes from the CE/J genome. We revealed that CE/J mice possess functional Saa1 and Saa2 genes. Intriguingly, the two genes were identical with respect to amino acid sequence, each encoding the SAA2.2 isoform. RT-PCR analysis of inflamed liver tissue from CE/J mice demonstrated that both genes are expressed at equivalent levels. 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In contrast to AA amyloidosis-susceptible mouse strains that concomitantly express serum amyloid A precursor protein (SAA) types 1 and 2 isoforms encoded by the Saa1 and Saa2 genes, respectively, in response to inflammatory stimulation from the liver, CE/J mice express only a single SAA isoform named SAA2.2. In addition, CE/J mice uniquely possess a Q30L amino acid substitution in SAA2.2 that inhibits amyloidogenesis. To elucidate the genetic basis underlying the expression of only a single SAA isoform in this strain, we conducted PCR cloning and nucleotide sequencing of the Saa1 and Saa2 genes from the CE/J genome. We revealed that CE/J mice possess functional Saa1 and Saa2 genes. Intriguingly, the two genes were identical with respect to amino acid sequence, each encoding the SAA2.2 isoform. RT-PCR analysis of inflamed liver tissue from CE/J mice demonstrated that both genes are expressed at equivalent levels. Reporter assays revealed that promoter/enhancer sequences of Saa1 and Saa2 genes in CE/J are also functional. These results indicate that the SAA2.2 isoform in CE/J is a mixture of Saa1 and Saa2 gene products."}]}, "item_creator": {"attribute_name": "\u8457\u8005", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Mori,  Masayuki", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "6248", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Tiang,  Geng", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "6249", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Higuchi,  Keiichi", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "6250", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "\u30d5\u30a1\u30a4\u30eb\u60c5\u5831", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2015-09-24"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "AA_amyloidosis-resistant_CE_J_mice.pdf", "filesize": [{"value": "696.2 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_note", "mimetype": "application/pdf", "size": 696200.0, "url": {"label": "AA_amyloidosis-resistant_CE_J_mice.pdf", "url": "https://soar-ir.repo.nii.ac.jp/record/3676/files/AA_amyloidosis-resistant_CE_J_mice.pdf"}, "version_id": "b3efec06-1ae6-436e-a3fe-de6a606c462e"}]}, "item_keyword": {"attribute_name": "\u30ad\u30fc\u30ef\u30fc\u30c9", "attribute_value_mlt": [{"subitem_subject": "Amino acid substitution", "subitem_subject_scheme": "Other"}, {"subitem_subject": "gene conversion", "subitem_subject_scheme": "Other"}, {"subitem_subject": "gene family", "subitem_subject_scheme": "Other"}, {"subitem_subject": "mouse strain", "subitem_subject_scheme": "Other"}, {"subitem_subject": "nucleotide substitution", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "\u8a00\u8a9e", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "AA amyloidosis-resistant CE/J mice have Saa1 and Saa2 genes that encode an identical SAA isoform", "item_titles": {"attribute_name": "\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_title": "AA amyloidosis-resistant CE/J mice have Saa1 and Saa2 genes that encode an identical SAA isoform", "subitem_title_language": "en"}]}, "item_type_id": "6", "owner": "1", "path": ["461/462"], "permalink_uri": "http://hdl.handle.net/10091/17837", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2014-09-05"}, "publish_date": "2014-09-05", "publish_status": "0", "recid": "3676", "relation": {}, "relation_version_is_last": true, "title": ["AA amyloidosis-resistant CE/J mice have Saa1 and Saa2 genes that encode an identical SAA isoform"], "weko_shared_id": -1}