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  1. 050 医学部, 大学院医学系研究科
  2. 0501 学術論文

A New High-Speed Droplet-Real-Time Polymerase Chain Reaction Method Can Detect Bovine Respiratory Syncytial Virus in Less than 10 Min

http://hdl.handle.net/10091/17830
03c5a7e0-ab0d-4087-b62c-d946968ef3d0
名前 / ファイル ライセンス アクション
New_High-Speed_Droplet-Real-Time_Polymerase_Chain.pdf New_High-Speed_Droplet-Real-Time_Polymerase_Chain.pdf (1.5 MB)
Item type 学術雑誌論文 / Journal Article(1)
公開日 2014-09-05
タイトル
言語 en
タイトル A New High-Speed Droplet-Real-Time Polymerase Chain Reaction Method Can Detect Bovine Respiratory Syncytial Virus in Less than 10 Min
言語
言語 eng
キーワード
主題Scheme Other
主題 bovine respiratory syncytial virus
キーワード
主題Scheme Other
主題 domestic animal
キーワード
主題Scheme Other
主題 droplet-real-time PCR
キーワード
主題Scheme Other
主題 infectious disease
資源タイプ
資源 http://purl.org/coar/resource_type/c_6501
タイプ journal article
著者 Uehara, Masayuki

× Uehara, Masayuki

WEKO 6270

en Uehara, Masayuki

Search repository
Matsuda, Kazuyuki

× Matsuda, Kazuyuki

WEKO 6271

en Matsuda, Kazuyuki

Search repository
Sugano, Mitsutoshi

× Sugano, Mitsutoshi

WEKO 6272

en Sugano, Mitsutoshi

Search repository
Honda, Takayuki

× Honda, Takayuki

WEKO 6273

en Honda, Takayuki

Search repository
信州大学研究者総覧へのリンク
氏名 Honda, Takayuki
URL http://soar-rd.shinshu-u.ac.jp/profile/ja.WeDUHFkh.html
出版者
出版者 JAPAN SOC VET SCI
引用
内容記述タイプ Other
内容記述 JOURNAL OF VETERINARY MEDICAL SCIENCE. 76(3):477-480 (2014)
書誌情報 JOURNAL OF VETERINARY MEDICAL SCIENCE

巻 76, 号 3, p. 477-480, 発行日 2014-03
抄録
内容記述タイプ Abstract
内容記述 The polymerase chain reaction (PCR) has been widely used for diagnosis of infectious diseases of domestic animals. Rapid detection of respiratory pathogens of cattle is useful for making therapeutic decisions. Therefore, we developed a new genetic-based method called droplet-real-time PCR, which can detect bovine respiratory syncytial virus (BRSV) within 10 min. Our droplet-real-time PCR markedly reduced the reaction time of reverse transcription-PCR while maintaining the same sensitivity as conventional real-time PCR, and it can be used as a rapid assay for detection of BRSV. Furthermore, our method is potentially applicable for rapid diagnosis of almost all infectious diseases, including highly pathogenic avian influenza virus.
資源タイプ(コンテンツの種類)
内容記述タイプ Other
内容記述 Article
ISSN
収録物識別子タイプ PISSN
収録物識別子 0916-7250
書誌レコードID
収録物識別子タイプ NCID
収録物識別子 AA10796138
PubMed
関連識別子
識別子タイプ PMID
関連識別子 https://pubmed.ncbi.nlm.nih.gov/24285011
関連名称
関連名称 24285011
DOI
関連識別子
識別子タイプ DOI
関連識別子 https://doi.org/10.1292/jvms.13-0357
関連名称
関連名称 10.1292/jvms.13-0357
権利
権利情報 Copyright© 2014 The Japanese Society of Veterinary Science
出版タイプ
出版タイプ VoR
出版タイプResource http://purl.org/coar/version/c_970fb48d4fbd8a85
WoS
表示名 Web of Science
URL http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=ShinshuUniv&SrcApp=ShinshuUniv&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000333777400025
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