Item type |
学術雑誌論文 / Journal Article(1) |
公開日 |
2014-09-05 |
タイトル |
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タイトル |
A New High-Speed Droplet-Real-Time Polymerase Chain Reaction Method Can Detect Bovine Respiratory Syncytial Virus in Less than 10 Min |
言語 |
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言語 |
eng |
DOI |
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関連識別子 |
https://doi.org/10.1292/jvms.13-0357 |
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関連名称 |
10.1292/jvms.13-0357 |
キーワード |
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主題 |
bovine respiratory syncytial virus, domestic animal, droplet-real-time PCR, infectious disease |
資源タイプ |
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資源 |
http://purl.org/coar/resource_type/c_6501 |
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タイプ |
journal article |
著者 |
Uehara, Masayuki
Matsuda, Kazuyuki
Sugano, Mitsutoshi
Honda, Takayuki
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信州大学研究者総覧へのリンク |
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氏名 |
Honda, Takayuki |
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URL |
http://soar-rd.shinshu-u.ac.jp/profile/ja.WeDUHFkh.html |
出版者 |
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出版者 |
JAPAN SOC VET SCI |
引用 |
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内容記述 |
JOURNAL OF VETERINARY MEDICAL SCIENCE. 76(3):477-480 (2014) |
書誌情報 |
JOURNAL OF VETERINARY MEDICAL SCIENCE
巻 76,
号 3,
p. 477-480,
発行日 2014-03
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抄録 |
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内容記述 |
The polymerase chain reaction (PCR) has been widely used for diagnosis of infectious diseases of domestic animals. Rapid detection of respiratory pathogens of cattle is useful for making therapeutic decisions. Therefore, we developed a new genetic-based method called droplet-real-time PCR, which can detect bovine respiratory syncytial virus (BRSV) within 10 min. Our droplet-real-time PCR markedly reduced the reaction time of reverse transcription-PCR while maintaining the same sensitivity as conventional real-time PCR, and it can be used as a rapid assay for detection of BRSV. Furthermore, our method is potentially applicable for rapid diagnosis of almost all infectious diseases, including highly pathogenic avian influenza virus. |
資源タイプ(コンテンツの種類) |
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内容記述 |
Article |
ISSN |
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収録物識別子タイプ |
PISSN |
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収録物識別子 |
0916-7250 |
書誌レコードID |
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収録物識別子タイプ |
NCID |
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収録物識別子 |
AA10796138 |
PubMed |
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関連識別子 |
https://pubmed.ncbi.nlm.nih.gov/24285011 |
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関連名称 |
24285011 |
権利 |
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権利情報 |
Copyright© 2014 The Japanese Society of Veterinary Science |
出版タイプ |
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出版タイプ |
VoR |
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出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
WoS |
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URL |
http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=ShinshuUniv&SrcApp=ShinshuUniv&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000333777400025 |