Item type |
学術雑誌論文 / Journal Article(1) |
公開日 |
2013-04-01 |
タイトル |
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タイトル |
Freeze-thawing single human embryonic stem cells induce e-cadherin and actin filament network disruption via g13 signaling |
言語 |
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言語 |
eng |
キーワード |
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主題 |
Cryopreservation, E-cadherin, actin filaments, G13 signaling pathway, human embryonic stem cells, ROCK inhibitor |
資源タイプ |
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資源 |
http://purl.org/coar/resource_type/c_6501 |
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タイプ |
journal article |
著者 |
Ichikawa, Hinako
Yoshie, Susumu
Shirasawa, Sakiko
Yokoyama, Tadayuki
Yue, Fengming
Tomotsune, Daihachiro
Sasaki, Katsunori
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信州大学研究者総覧へのリンク |
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氏名 |
Tomotsune, Daihachiro |
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URL |
http://soar-rd.shinshu-u.ac.jp/profile/ja.OekCuNkh.html |
出版者 |
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出版者 |
CRYO LETTERS |
引用 |
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内容記述 |
CRYO LETTERS. 32(6):516-524 (2011) |
書誌情報 |
CRYO LETTERS
巻 32,
号 6,
p. 516-524,
発行日 2011
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抄録 |
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内容記述 |
Poor adhesion of single human embryonic stem (hES) cells after freeze-thawing causes death. To investigate mechanisms responsible for this, Rho-dependent protein kinase (ROCK) inhibitor Y-27632-treated and untreated single hES cells were analyzed for E-cadherin and F-actin distribution by immunostaining and phalloidin staining respectively and for G13 signaling pathway components by DNA microarray and quantitative polymerase chain reaction (PCR). Y-27632-treated cells clustered rapidly and maintained E-cadherin and F-actin distribution without losing Oct3/4. Immediately after thawing, E-cadherin in untreated hES cells dotted along the membrane and then displayed eccentric cytoplasmic localization. Bleb formation and early Oct3/4 loss occurred after F-actin network condensation in the cytoplasm. Microarray analyses and quantitative PCR indicated upregulation of two actin reorganization-associated components of the G13 signaling pathway, Arhgdib and Cdc42, in untreated cells. Considering these findings and that cell death was partly interrupted by Y-27632, E-cadherin and actin cytoskeleton network disruption through the G13 signaling pathway may cause hES cell death after freeze-thawing. |
資源タイプ(コンテンツの種類) |
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内容記述 |
Article |
ISSN |
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収録物識別子タイプ |
PISSN |
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収録物識別子 |
0143-2044 |
書誌レコードID |
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収録物識別子タイプ |
NCID |
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収録物識別子 |
AA00145687 |
他の資源との関係:URI |
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関連識別子 |
http://www.cryoletters.org/ |
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関連名称 |
http://www.cryoletters.org/ |
PubMed |
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関連識別子 |
https://pubmed.ncbi.nlm.nih.gov/22227712 |
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関連名称 |
22227712 |
権利 |
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権利情報 |
Copyright© CryoLetters |
出版タイプ |
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出版タイプ |
VoR |
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出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
WoS |
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URL |
http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=ShinshuUniv&SrcApp=ShinshuUniv&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000298622900009 |