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Freeze-thawing single human embryonic stem cells induce e-cadherin and actin filament network disruption via g13 signaling
http://hdl.handle.net/10091/16881
http://hdl.handle.net/10091/168814897359e-9695-4cf7-aebc-b5817dc15949
名前 / ファイル | ライセンス | アクション |
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Cryoletters32.6.pp516-524.pdf (689.6 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2013-04-01 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Freeze-thawing single human embryonic stem cells induce e-cadherin and actin filament network disruption via g13 signaling | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題 | Cryopreservation, E-cadherin, actin filaments, G13 signaling pathway, human embryonic stem cells, ROCK inhibitor | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | journal article | |||||
著者 |
Ichikawa, Hinako
× Ichikawa, Hinako× Yoshie, Susumu× Shirasawa, Sakiko× Yokoyama, Tadayuki× Yue, Fengming× Tomotsune, Daihachiro× Sasaki, Katsunori |
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信州大学研究者総覧へのリンク | ||||||
氏名 | Tomotsune, Daihachiro | |||||
URL | http://soar-rd.shinshu-u.ac.jp/profile/ja.OekCuNkh.html | |||||
出版者 | ||||||
出版者 | CRYO LETTERS | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | CRYO LETTERS. 32(6):516-524 (2011) | |||||
書誌情報 |
CRYO LETTERS 巻 32, 号 6, p. 516-524, 発行日 2011 |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Poor adhesion of single human embryonic stem (hES) cells after freeze-thawing causes death. To investigate mechanisms responsible for this, Rho-dependent protein kinase (ROCK) inhibitor Y-27632-treated and untreated single hES cells were analyzed for E-cadherin and F-actin distribution by immunostaining and phalloidin staining respectively and for G13 signaling pathway components by DNA microarray and quantitative polymerase chain reaction (PCR). Y-27632-treated cells clustered rapidly and maintained E-cadherin and F-actin distribution without losing Oct3/4. Immediately after thawing, E-cadherin in untreated hES cells dotted along the membrane and then displayed eccentric cytoplasmic localization. Bleb formation and early Oct3/4 loss occurred after F-actin network condensation in the cytoplasm. Microarray analyses and quantitative PCR indicated upregulation of two actin reorganization-associated components of the G13 signaling pathway, Arhgdib and Cdc42, in untreated cells. Considering these findings and that cell death was partly interrupted by Y-27632, E-cadherin and actin cytoskeleton network disruption through the G13 signaling pathway may cause hES cell death after freeze-thawing. | |||||
資源タイプ(コンテンツの種類) | ||||||
内容記述タイプ | Other | |||||
内容記述 | Article | |||||
ISSN | ||||||
収録物識別子タイプ | PISSN | |||||
収録物識別子 | 0143-2044 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA00145687 | |||||
他の資源との関係:URI | ||||||
識別子タイプ | URI | |||||
関連識別子 | http://www.cryoletters.org/ | |||||
関連名称 | http://www.cryoletters.org/ | |||||
PubMed | ||||||
識別子タイプ | PMID | |||||
関連識別子 | https://pubmed.ncbi.nlm.nih.gov/22227712 | |||||
関連名称 | 22227712 | |||||
権利 | ||||||
権利情報 | Copyright© CryoLetters | |||||
出版タイプ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
WoS | ||||||
表示名 | Web of Science | |||||
URL | http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=ShinshuUniv&SrcApp=ShinshuUniv&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000298622900009 |