{"_buckets": {"deposit": "3fc280ed-04b6-4859-a9d6-ab84bdb0f7ee"}, "_deposit": {"id": "3828", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "3828"}, "status": "published"}, "_oai": {"id": "oai:soar-ir.repo.nii.ac.jp:00003828"}, "item_6_biblio_info_6": {"attribute_name": "\u66f8\u8a8c\u60c5\u5831", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2011-06", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "6", "bibliographicPageEnd": "583", "bibliographicPageStart": "572", "bibliographicVolumeNumber": "59", "bibliographic_titles": [{"bibliographic_title": "JOURNAL OF HISTOCHEMISTRY \u0026 CYTOCHEMISTRY"}]}]}, "item_6_description_20": {"attribute_name": "\u6284\u9332", "attribute_value_mlt": [{"subitem_description": "Lymphocyte homing is regulated by a multistep process mediated by sequential adhesive interactions between circulating lymphocytes and high endothelial venules (HEVs). In gut-associated lymphoid tissue (GALT), the initial interactive step, \"tethering and rolling,\" is partly mediated by integrin alpha 4 beta 7 expressed on GALT-homing lymphocytes and its ligand MAdCAM-1, which is exclusively expressed on HEVs in GALT. To probe functional MAdCAM-1 in tissue sections, we developed a soluble integrin alpha 4 beta 7 heterodimeric IgG chimera by joining the extracellular region of mouse integrin alpha 4 and beta 7 subunits to a human IgG Fc domain. Western blot analysis revealed that co-transfection of HEK 293T cells with expression vectors encoding integrin alpha 4.IgG and beta 7.IgG results in the formation of alpha 4 beta 7.IgG heterodimeric chimeras. This complex preferentially binds to CHO cells expressing MAdCAM-1 and, to a lesser extent, to cells expressing VCAM-1, but not to cells expressing ICAM-1. Moreover, alpha 4 beta 7.IgG specifically binds to HEVs in GALT in situ in a divalent cation-dependent fashion and inhibits lymphocyte binding to HEVs in GALT. These findings indicate that alpha 4 beta 7.IgG can be used as a probe for functional MAdCAM-1 expressed on HEVs in GALT and could potentially serve as an anti-inflammatory drug inhibiting GALT-specific lymphocyte migration. 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In gut-associated lymphoid tissue (GALT), the initial interactive step, \"tethering and rolling,\" is partly mediated by integrin alpha 4 beta 7 expressed on GALT-homing lymphocytes and its ligand MAdCAM-1, which is exclusively expressed on HEVs in GALT. To probe functional MAdCAM-1 in tissue sections, we developed a soluble integrin alpha 4 beta 7 heterodimeric IgG chimera by joining the extracellular region of mouse integrin alpha 4 and beta 7 subunits to a human IgG Fc domain. Western blot analysis revealed that co-transfection of HEK 293T cells with expression vectors encoding integrin alpha 4.IgG and beta 7.IgG results in the formation of alpha 4 beta 7.IgG heterodimeric chimeras. This complex preferentially binds to CHO cells expressing MAdCAM-1 and, to a lesser extent, to cells expressing VCAM-1, but not to cells expressing ICAM-1. Moreover, alpha 4 beta 7.IgG specifically binds to HEVs in GALT in situ in a divalent cation-dependent fashion and inhibits lymphocyte binding to HEVs in GALT. These findings indicate that alpha 4 beta 7.IgG can be used as a probe for functional MAdCAM-1 expressed on HEVs in GALT and could potentially serve as an anti-inflammatory drug inhibiting GALT-specific lymphocyte migration. (J Histochem Cytochem 59:572-583, 2011)"}]}, "item_creator": {"attribute_name": "\u8457\u8005", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Hoshino,  Hitomi"}], "nameIdentifiers": [{"nameIdentifier": "7334", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kobayashi,  Motohiro"}], "nameIdentifiers": [{"nameIdentifier": "7335", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Mitoma,  Junya"}], "nameIdentifiers": [{"nameIdentifier": "7336", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Sato,  Yoshiko"}], "nameIdentifiers": [{"nameIdentifier": "7337", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Fukuda,  Minoru"}], "nameIdentifiers": [{"nameIdentifier": "7338", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Nakayama,  Jun"}], "nameIdentifiers": [{"nameIdentifier": "7339", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "\u30d5\u30a1\u30a4\u30eb\u60c5\u5831", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2015-09-24"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "An_Integrin_alpha_4_beta_7_IgG_Heterodimeric_Chimera.pdf", "filesize": [{"value": "932.5 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 932500.0, "url": {"label": "An_Integrin_alpha_4_beta_7_IgG_Heterodimeric_Chimera.pdf", "url": "https://soar-ir.repo.nii.ac.jp/record/3828/files/An_Integrin_alpha_4_beta_7_IgG_Heterodimeric_Chimera.pdf"}, "version_id": "af94da54-3100-4753-9eb5-36e4a7309a9e"}]}, "item_keyword": {"attribute_name": "\u30ad\u30fc\u30ef\u30fc\u30c9", "attribute_value_mlt": [{"subitem_subject": "alpha 4 beta 7 integrin", "subitem_subject_scheme": "Other"}, {"subitem_subject": "functional probe", "subitem_subject_scheme": "Other"}, {"subitem_subject": "gut-associated lymphoid tissue (GALT)", "subitem_subject_scheme": "Other"}, {"subitem_subject": "high endothelial venule (HEV)", "subitem_subject_scheme": "Other"}, {"subitem_subject": "lymphocyte homing", "subitem_subject_scheme": "Other"}, {"subitem_subject": "mucosal addressin cell adhesion molecule 1 (MAdCAM-1)", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "\u8a00\u8a9e", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "An Integrin alpha 4 beta 7.IgG Heterodimeric Chimera Binds to MAdCAM-1 on High Endothelial Venules in Gut-Associated Lymphoid Tissue", "item_titles": {"attribute_name": "\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_title": "An Integrin alpha 4 beta 7.IgG Heterodimeric Chimera Binds to MAdCAM-1 on High Endothelial Venules in Gut-Associated Lymphoid Tissue"}]}, "item_type_id": "6", "owner": "1", "path": ["461/462"], "permalink_uri": "http://hdl.handle.net/10091/16235", "pubdate": {"attribute_name": "\u516c\u958b\u65e5", "attribute_name_i18n": "\u516c\u958b\u65e5", "attribute_value": "2013-02-04"}, "publish_date": "2013-02-04", "publish_status": "0", "recid": "3828", "relation": {}, "relation_version_is_last": true, "title": ["An Integrin alpha 4 beta 7.IgG Heterodimeric Chimera Binds to MAdCAM-1 on High Endothelial Venules in Gut-Associated Lymphoid Tissue"], "weko_shared_id": null}