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An Integrin alpha 4 beta 7.IgG Heterodimeric Chimera Binds to MAdCAM-1 on High Endothelial Venules in Gut-Associated Lymphoid Tissue
http://hdl.handle.net/10091/16235
http://hdl.handle.net/10091/162353441b4da-0840-4187-a75f-aec58480af78
名前 / ファイル | ライセンス | アクション |
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Item type | 学術雑誌論文 / Journal Article(1) | |||||||||||||||||
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公開日 | 2013-02-04 | |||||||||||||||||
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タイトル | An Integrin alpha 4 beta 7.IgG Heterodimeric Chimera Binds to MAdCAM-1 on High Endothelial Venules in Gut-Associated Lymphoid Tissue | |||||||||||||||||
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言語 | eng | |||||||||||||||||
DOI | ||||||||||||||||||
関連識別子 | https://doi.org/10.1369/0022155411404416 | |||||||||||||||||
関連名称 | 10.1369/0022155411404416 | |||||||||||||||||
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主題 | alpha 4 beta 7 integrin, functional probe, gut-associated lymphoid tissue (GALT), high endothelial venule (HEV), lymphocyte homing, mucosal addressin cell adhesion molecule 1 (MAdCAM-1) | |||||||||||||||||
資源タイプ | ||||||||||||||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||||||
タイプ | journal article | |||||||||||||||||
著者 |
Hoshino, Hitomi
× Hoshino, Hitomi
× Kobayashi, Motohiro
× Mitoma, Junya
× Sato, Yoshiko
× Fukuda, Minoru
× Nakayama, Jun
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信州大学研究者総覧へのリンク | ||||||||||||||||||
氏名 | Nakayama, Jun | |||||||||||||||||
URL | http://soar-rd.shinshu-u.ac.jp/profile/ja.HCApHFkh.html | |||||||||||||||||
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出版者 | SAGE PUBLICATIONS LTD | |||||||||||||||||
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内容記述 | JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY. 59(6):572-583 (2011) | |||||||||||||||||
書誌情報 |
JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY 巻 59, 号 6, p. 572-583, 発行日 2011-06 |
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内容記述 | Lymphocyte homing is regulated by a multistep process mediated by sequential adhesive interactions between circulating lymphocytes and high endothelial venules (HEVs). In gut-associated lymphoid tissue (GALT), the initial interactive step, "tethering and rolling," is partly mediated by integrin alpha 4 beta 7 expressed on GALT-homing lymphocytes and its ligand MAdCAM-1, which is exclusively expressed on HEVs in GALT. To probe functional MAdCAM-1 in tissue sections, we developed a soluble integrin alpha 4 beta 7 heterodimeric IgG chimera by joining the extracellular region of mouse integrin alpha 4 and beta 7 subunits to a human IgG Fc domain. Western blot analysis revealed that co-transfection of HEK 293T cells with expression vectors encoding integrin alpha 4.IgG and beta 7.IgG results in the formation of alpha 4 beta 7.IgG heterodimeric chimeras. This complex preferentially binds to CHO cells expressing MAdCAM-1 and, to a lesser extent, to cells expressing VCAM-1, but not to cells expressing ICAM-1. Moreover, alpha 4 beta 7.IgG specifically binds to HEVs in GALT in situ in a divalent cation-dependent fashion and inhibits lymphocyte binding to HEVs in GALT. These findings indicate that alpha 4 beta 7.IgG can be used as a probe for functional MAdCAM-1 expressed on HEVs in GALT and could potentially serve as an anti-inflammatory drug inhibiting GALT-specific lymphocyte migration. (J Histochem Cytochem 59:572-583, 2011) | |||||||||||||||||
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収録物識別子タイプ | PISSN | |||||||||||||||||
収録物識別子 | 0022-1554 | |||||||||||||||||
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収録物識別子タイプ | NCID | |||||||||||||||||
収録物識別子 | AA0069926X | |||||||||||||||||
PubMed | ||||||||||||||||||
識別子タイプ | PMID | |||||||||||||||||
関連識別子 | https://pubmed.ncbi.nlm.nih.gov/21430257 | |||||||||||||||||
関連名称 | 21430257 | |||||||||||||||||
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出版タイプ | AM | |||||||||||||||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||||||||||||||
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URL | http://gateway.isiknowledge.com/gateway/Gateway.cgi?&GWVersion=2&SrcAuth=ShinshuUniv&SrcApp=ShinshuUniv&DestLinkType=FullRecord&DestApp=WOS&KeyUT=000293703900001 |
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Hoshino, Hitomi, Kobayashi, Motohiro, Mitoma, Junya, Sato, Yoshiko, Fukuda, Minoru, Nakayama, Jun, 2011, An Integrin alpha 4 beta 7.IgG Heterodimeric Chimera Binds to MAdCAM-1 on High Endothelial Venules in Gut-Associated Lymphoid Tissue: SAGE PUBLICATIONS LTD, 572–583 p.
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