{"_buckets": {"deposit": "5828bbd1-31cd-4d2d-9b84-0e33ed7d9f8a"}, "_deposit": {"id": "3913", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "3913"}, "status": "published"}, "_oai": {"id": "oai:soar-ir.repo.nii.ac.jp:00003913", "sets": ["462"]}, "author_link": ["7950", "7951", "7952", "7953", "7954", "7955", "7956", "7957", "7958"], "item_1628147817048": {"attribute_name": "出版タイプ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_ab4af688f83e57aa", "subitem_version_type": "AM"}]}, "item_6_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2009-10", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "10", "bibliographicPageEnd": "1529", "bibliographicPageStart": "1517", "bibliographicVolumeNumber": "1794", "bibliographic_titles": [{"bibliographic_title": "BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS"}]}]}, "item_6_description_20": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "In mice, amyloidogenic type C apolipoprotein A-II (apoA-II) forms amyloid fibrils in age-associated amyloidosis. To understand the mechanism of amyloid fibril formation by apoA-II, we examined the polymerization of synthetic partial peptides of apoA-II in vitro. None of the partial apoA-II peptides polymerized into amyloid fibrils when tested as a single species mixture. We found a unique mechanism in which N- and C-terminal peptides associated into amyloid fibrils in a 1:1 ratio at pH 2.5. The 11-residue amino acid sequence (6-16), which is a common sequence of type B apoA-II and type C apoA-II proteins in amyloidosis-resistant mice and amyloidosis-susceptible mice, respectively, was critical for polymerization into amyloid fibrils. The 18-residue-long amino acid sequence (48-65) is also necessary for nucleation, but not for the extension phase. These findings suggest that there may be different mechanisms underlying the nucleation and extension phases of apoA-II amyloid fibril formation. We also found that amino acid substitutions between type B apoA-II (Pro5, Val38) and type C apoA-II (Gln5, Ala38) did not affect either phase. The strategy of using synthetic partial peptides of amyloidogenic proteins in vitro is a useful system for understanding amyloid fibril formation and for the development of novel therapies.", "subitem_description_type": "Abstract"}]}, "item_6_description_30": {"attribute_name": "資源タイプ（コンテンツの種類）", "attribute_value_mlt": [{"subitem_description": "Article", "subitem_description_type": "Other"}]}, "item_6_description_31": {"attribute_name": "フォーマット：mimeタイプ", "attribute_value_mlt": [{"subitem_description": "application/pdf", "subitem_description_type": "Other"}]}, "item_6_description_5": {"attribute_name": "引用", "attribute_value_mlt": [{"subitem_description": "BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS. 1794(10):1517-1529 (2009)", "subitem_description_type": "Other"}]}, "item_6_link_3": {"attribute_name": "信州大学研究者総覧へのリンク", "attribute_value_mlt": [{"subitem_link_text": "Mori, Masayuki", "subitem_link_url": "http://soar-rd.shinshu-u.ac.jp/profile/ja.OpSCZafV.html"}, {"subitem_link_text": "Higuchi, Keiichi", "subitem_link_url": "http://soar-rd.shinshu-u.ac.jp/profile/ja.gCfUPmAh.html"}]}, "item_6_link_67": {"attribute_name": "WoS", "attribute_value_mlt": [{"subitem_link_text": "Web of Science", "subitem_link_url": "http://gateway.isiknowledge.com/gateway/Gateway.cgi?\u0026GWVersion=2\u0026SrcAuth=ShinshuUniv\u0026SrcApp=ShinshuUniv\u0026DestLinkType=FullRecord\u0026DestApp=WOS\u0026KeyUT=000270259000017"}]}, "item_6_publisher_4": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "ELSEVIER SCIENCE BV"}]}, "item_6_relation_47": {"attribute_name": "PubMed", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "19596087"}], "subitem_relation_type_id": {"subitem_relation_type_id_text": "https://pubmed.ncbi.nlm.nih.gov/19596087", "subitem_relation_type_select": "PMID"}}]}, "item_6_relation_48": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "10.1016/j.bbapap.2009.06.028"}], "subitem_relation_type_id": {"subitem_relation_type_id_text": "https://doi.org/10.1016/j.bbapap.2009.06.028", "subitem_relation_type_select": "DOI"}}]}, "item_6_rights_62": {"attribute_name": "権利", "attribute_value_mlt": [{"subitem_rights": "Copyright (c) 2009 Elsevier B.V."}]}, "item_6_select_64": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_select_item": "author"}]}, "item_6_source_id_35": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "1570-9639", "subitem_source_identifier_type": "PISSN"}]}, "item_6_source_id_39": {"attribute_name": "NII ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "1570-9639", "subitem_source_identifier_type": "PISSN"}]}, "item_6_source_id_40": {"attribute_name": "書誌レコードID", "attribute_value_mlt": [{"subitem_source_identifier": "AA11685085", "subitem_source_identifier_type": "NCID"}]}, "item_6_text_69": {"attribute_name": "wosonly authkey", "attribute_value_mlt": [{"subitem_text_value": "Apolipoprotein A-II; Mouse senile amyloidosis; Fibril conformation"}]}, "item_6_text_70": {"attribute_name": "wosonly keywords", "attribute_value_mlt": [{"subitem_text_value": "HIGH-DENSITY-LIPOPROTEINS; SENILE AMYLOIDOSIS; APOA-II; BETA-PEPTIDE; NEUTRAL PH; IN-VITRO; ALZHEIMERS-DISEASE; TRANSGENIC MICE; INBRED STRAINS; AGGREGATION"}]}, "item_6_textarea_68": {"attribute_name": "wosonly abstract", "attribute_value_mlt": [{"subitem_textarea_value": "In mice, amyloidogenic type C apolipoprotein A-II (apoA-II) forms amyloid fibrils in age-associated amyloidosis. To understand the mechanism of amyloid fibril formation by apoA-II, we examined the polymerization of synthetic partial peptides of apoA-II in vitro. None of the partial apoA-II peptides polymerized into amyloid fibrils when tested as a single species mixture. We found a unique mechanism in which N- and C-terminal peptides associated into amyloid fibrils in a 1:1 ratio at pH 2.5. The 11-residue amino acid sequence (6-16), which is a common sequence of type B apoA-II and type C apoA-II proteins in amyloidosis-resistant mice and amyloidosis-susceptible mice, respectively, was critical for polymerization into amyloid fibrils. The 18-residue-long amino acid sequence (48-65) is also necessary for nucleation, but not for the extension phase. These findings suggest that there may be different mechanisms underlying the nucleation and extension phases of apoA-II amyloid fibril formation. We also found that amino acid substitutions between type B apoA-II (Pro5, Val38) and type C apoA-II (Gln5, Ala38) did not affect either phase. The strategy of using synthetic partial peptides of amyloidogenic proteins in vitro is a useful system for understanding amyloid fibril formation and for the development of novel therapies. (C) 2009 Elsevier B.V. All rights reserved."}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Sawashita,  Jinko", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "7950", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kametani,  Fuyuki", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "7951", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Hasegawa,  Kazuhiro", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "7952", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Tsutsumi-Yasuhara,  Shinobu", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "7953", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Zhang,  Beiru", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "7954", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Yan,  Jingmin", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "7955", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Mori,  Masayuki", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "7956", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Naiki,  Hironobu", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "7957", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Higuchi,  Keiichi", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "7958", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2015-09-24"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "Amyloid_fibrils_formed_selective_N-C-terminal.pdf", "filesize": [{"value": "901.5 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_note", "mimetype": "application/pdf", "size": 901500.0, "url": {"label": "Amyloid_fibrils_formed_selective_N-C-terminal.pdf", "url": "https://soar-ir.repo.nii.ac.jp/record/3913/files/Amyloid_fibrils_formed_selective_N-C-terminal.pdf"}, "version_id": "55c2c40a-9225-4513-bf4d-8c6c619d1fb5"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "Apolipoprotein A-II", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Mouse senile amyloidosis", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Fibril conformation", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Amyloid fibrils formed by selective N-, C-terminal sequences of mouse apolipoprotein A-II", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Amyloid fibrils formed by selective N-, C-terminal sequences of mouse apolipoprotein A-II", "subitem_title_language": "en"}]}, "item_type_id": "6", "owner": "1", "path": ["462"], "permalink_uri": "http://hdl.handle.net/10091/10791", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2010-11-18"}, "publish_date": "2010-11-18", "publish_status": "0", "recid": "3913", "relation": {}, "relation_version_is_last": true, "title": ["Amyloid fibrils formed by selective N-, C-terminal sequences of mouse apolipoprotein A-II"], "weko_shared_id": -1}