{"_buckets": {"deposit": "8b5760b2-c802-422c-b546-8f5fd848406b"}, "_deposit": {"id": "4169", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "4169"}, "status": "published"}, "_oai": {"id": "oai:soar-ir.repo.nii.ac.jp:00004169", "sets": []}, "author_link": ["9460", "9461", "9462", "9463", "9464", "9465", "9466", "9467", "9468", "9469", "9470"], "item_10_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2011-02-10", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "1", "bibliographicPageEnd": "26", "bibliographicPageStart": "13", "bibliographicVolumeNumber": "59", "bibliographic_titles": [{"bibliographic_title": "信州医学雑誌"}]}]}, "item_10_description_20": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "We analyzed Ca-permeating nonselective cation channels (NSCs)mediating thrombin-induced contraction of human umbilical vein endothelial cells (HUVECs). A Ca chelater, BAPTA-AM (10μM), significantly inhibited the thrombin-induced contraction of HUVECs.Thrombin induced inward currents at -60 mV in the presence of intracellular MgATP. Removal of extracellular Caﾌﾞｸﾞsignificantly decreased the currents. A selective phospholipase C inhibitor, U73122 (1μM) but not its inactive analogue, U73343 (1μM) almost completely inhibited the currents. Neither a selective inhibitor of Caﾌﾞｸﾞ-ATPase of endoplasmic reticulum, thapsigargin (1μM)nor a diacylglycerol analogue, 1-oleoyl-2-acetyl-glycerol (30μM)activated the currents. However, a selective protein kinase C inhibitor, bisindolylmaleimide I (500 nM) significantly inhibited the currents.The thrombin-induced currents were significantly inhibited by SKF96365 (50μM)but not by La(1mM), ruthenium red (10μM) or flufenamic acid (100μM). As assessed with RT-PCR, HUVECs expressed transient receptor potential(TRP)M4,7,TRPV1,2,4,TRPC1,4 and 6 subunits of NSCs.These results indicate that thrombin activates Ca-permeating NSCs containing TRPC4 through protein kinase C in HUVECs. Thus,drugs specifically inhibiting TRPC4-containing channels might be effective to control fatal diseases such as sepsis where thrombin mediates the vicious cycle between inflammation and coagulation.", "subitem_description_type": "Abstract"}]}, "item_10_description_30": {"attribute_name": "資源タイプ（コンテンツの種類）", "attribute_value_mlt": [{"subitem_description": "Article", "subitem_description_type": "Other"}]}, "item_10_description_31": {"attribute_name": "フォーマット：mimeタイプ", "attribute_value_mlt": [{"subitem_description": "application/pdf", "subitem_description_type": "Other"}]}, "item_10_description_5": {"attribute_name": "引用", "attribute_value_mlt": [{"subitem_description": "信州医学雑誌 59(1): 13-26(2011)", "subitem_description_type": "Other"}]}, "item_10_link_3": {"attribute_name": "信州大学研究者総覧へのリンク", "attribute_value_mlt": [{"subitem_link_text": "OHHASHI, Toshio", "subitem_link_url": "http://soar-rd.shinshu-u.ac.jp/profile/ja.HFApOFkh.html"}, {"subitem_link_text": "YAMADA, Mitsuhiko", "subitem_link_url": "http://soar-rd.shinshu-u.ac.jp/profile/ja.jhSCupca.html"}]}, "item_10_publisher_4": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "信州医学会"}]}, "item_10_relation_48": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "10.11441/shinshumedj.59.13"}], "subitem_relation_type": "isIdenticalTo", "subitem_relation_type_id": {"subitem_relation_type_id_text": "https://doi.org/10.11441/shinshumedj.59.13", "subitem_relation_type_select": "DOI"}}]}, "item_10_select_64": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_select_item": "publisher"}]}, "item_10_source_id_35": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "0037-3826", "subitem_source_identifier_type": "PISSN"}]}, "item_10_source_id_39": {"attribute_name": "NII ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "0037-3826", "subitem_source_identifier_type": "PISSN"}]}, 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[{"nameIdentifier": "9462", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "KOBAYASHI, Masaaki", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "9463", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "KAWAI, Yoshiko", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "9464", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "YANO, Shiharu", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "9465", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "ATSUNAGA, Tamihide", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "9466", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "HIROSE, Masamichi", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "9467", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "OHMORI, Shigeru", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "9468", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "OHHASHI,  Toshio", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "9469", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "YAMADA,  Mitsuhiko", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "9470", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2015-09-24"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "Shinshu_med59-1-02.pdf", "filesize": [{"value": "991.5 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_note", "mimetype": "application/pdf", "size": 991500.0, "url": {"label": "Shinshu_med59-1-02.pdf", "url": "https://soar-ir.repo.nii.ac.jp/record/4169/files/Shinshu_med59-1-02.pdf"}, "version_id": "59265691-bb54-408c-a8c9-5c29af41f41c"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "human umbilical vein endothelial cells", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "thrombin", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "nonselective cation channels", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "transient receptor potential C4", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "protein kinase C", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Thrombin Activates Ca2+-permeating Nonselective Cation Channels through Protein Kinase C in Human Umbilical Vein Endothelial Cells", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Thrombin Activates Ca2+-permeating Nonselective Cation Channels through Protein Kinase C in Human Umbilical Vein Endothelial Cells", "subitem_title_language": "en"}]}, "item_type_id": "10", "owner": "1", "path": ["492"], "permalink_uri": "http://hdl.handle.net/10091/11716", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2011-02-15"}, "publish_date": "2011-02-15", "publish_status": "0", "recid": "4169", "relation": {}, "relation_version_is_last": true, "title": ["Thrombin Activates Ca2+-permeating Nonselective Cation Channels through Protein Kinase C in Human Umbilical Vein Endothelial Cells"], "weko_shared_id": -1}