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Thrombin Activates Ca2+-permeating Nonselective Cation Channels through Protein Kinase C in Human Umbilical Vein Endothelial Cells
http://hdl.handle.net/10091/11716
http://hdl.handle.net/10091/11716135a3868-e6ec-4907-97ec-0acea61f8160
| 名前 / ファイル | ライセンス | アクション |
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Shinshu_med59-1-02.pdf (991.5 kB)
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| Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||||||||||||||||||||||||
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| 公開日 | 2011-02-15 | |||||||||||||||||||||||||||
| タイトル | ||||||||||||||||||||||||||||
| タイトル | Thrombin Activates Ca2+-permeating Nonselective Cation Channels through Protein Kinase C in Human Umbilical Vein Endothelial Cells | |||||||||||||||||||||||||||
| 言語 | ||||||||||||||||||||||||||||
| 言語 | eng | |||||||||||||||||||||||||||
| DOI | ||||||||||||||||||||||||||||
| 関連タイプ | isIdenticalTo | |||||||||||||||||||||||||||
| 関連識別子 | https://doi.org/10.11441/shinshumedj.59.13 | |||||||||||||||||||||||||||
| 関連名称 | 10.11441/shinshumedj.59.13 | |||||||||||||||||||||||||||
| キーワード | ||||||||||||||||||||||||||||
| 主題 | human umbilical vein endothelial cells, thrombin, nonselective cation channels, transient receptor potential C4, protein kinase C | |||||||||||||||||||||||||||
| 資源タイプ | ||||||||||||||||||||||||||||
| 資源 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||||||||||||||||
| タイプ | departmental bulletin paper | |||||||||||||||||||||||||||
| 著者 |
TSUJINO, Natsuko
× TSUJINO, Natsuko
× NAKADA, Tsutomu
× TSUBOUCHI, Kiyotaka
× KOBAYASHI, Masaaki
× KAWAI, Yoshiko
× YANO, Shiharu
× ATSUNAGA, Tamihide
× HIROSE, Masamichi
× OHMORI, Shigeru
× OHHASHI, Toshio
× YAMADA, Mitsuhiko
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| 信州大学研究者総覧へのリンク | ||||||||||||||||||||||||||||
| 氏名 | OHHASHI, Toshio | |||||||||||||||||||||||||||
| URL | http://soar-rd.shinshu-u.ac.jp/profile/ja.HFApOFkh.html | |||||||||||||||||||||||||||
| 信州大学研究者総覧へのリンク | ||||||||||||||||||||||||||||
| 氏名 | YAMADA, Mitsuhiko | |||||||||||||||||||||||||||
| URL | http://soar-rd.shinshu-u.ac.jp/profile/ja.jhSCupca.html | |||||||||||||||||||||||||||
| 出版者 | ||||||||||||||||||||||||||||
| 出版者 | 信州医学会 | |||||||||||||||||||||||||||
| 引用 | ||||||||||||||||||||||||||||
| 内容記述 | 信州医学雑誌 59(1): 13-26(2011) | |||||||||||||||||||||||||||
| 書誌情報 |
信州医学雑誌 巻 59, 号 1, p. 13-26, 発行日 2011-02-10 |
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| 抄録 | ||||||||||||||||||||||||||||
| 内容記述 | We analyzed Ca-permeating nonselective cation channels (NSCs)mediating thrombin-induced contraction of human umbilical vein endothelial cells (HUVECs). A Ca chelater, BAPTA-AM (10μM), significantly inhibited the thrombin-induced contraction of HUVECs.Thrombin induced inward currents at -60 mV in the presence of intracellular MgATP. Removal of extracellular Caﾌﾞｸﾞsignificantly decreased the currents. A selective phospholipase C inhibitor, U73122 (1μM) but not its inactive analogue, U73343 (1μM) almost completely inhibited the currents. Neither a selective inhibitor of Caﾌﾞｸﾞ-ATPase of endoplasmic reticulum, thapsigargin (1μM)nor a diacylglycerol analogue, 1-oleoyl-2-acetyl-glycerol (30μM)activated the currents. However, a selective protein kinase C inhibitor, bisindolylmaleimide I (500 nM) significantly inhibited the currents.The thrombin-induced currents were significantly inhibited by SKF96365 (50μM)but not by La(1mM), ruthenium red (10μM) or flufenamic acid (100μM). As assessed with RT-PCR, HUVECs expressed transient receptor potential(TRP)M4,7,TRPV1,2,4,TRPC1,4 and 6 subunits of NSCs.These results indicate that thrombin activates Ca-permeating NSCs containing TRPC4 through protein kinase C in HUVECs. Thus,drugs specifically inhibiting TRPC4-containing channels might be effective to control fatal diseases such as sepsis where thrombin mediates the vicious cycle between inflammation and coagulation. | |||||||||||||||||||||||||||
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| 収録物識別子タイプ | PISSN | |||||||||||||||||||||||||||
| 収録物識別子 | 0037-3826 | |||||||||||||||||||||||||||
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| 収録物識別子タイプ | NCID | |||||||||||||||||||||||||||
| 収録物識別子 | AN00120815 | |||||||||||||||||||||||||||
| 出版タイプ | ||||||||||||||||||||||||||||
| 出版タイプ | VoR | |||||||||||||||||||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||||||||||||||||||
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TSUJINO, Natsuko, NAKADA, Tsutomu, TSUBOUCHI, Kiyotaka, KOBAYASHI, Masaaki, KAWAI, Yoshiko, YANO, Shiharu, ATSUNAGA, Tamihide, HIROSE, Masamichi, OHMORI, Shigeru, OHHASHI, Toshio, YAMADA, Mitsuhiko, 2011, Thrombin Activates Ca2+-permeating Nonselective Cation Channels through Protein Kinase C in Human Umbilical Vein Endothelial Cells: 信州医学会, 13–26 p.
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