{"_buckets": {"deposit": "c428f87a-feec-486e-9017-942124757774"}, "_deposit": {"id": "7189", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "7189"}, "status": "published"}, "_oai": {"id": "oai:soar-ir.repo.nii.ac.jp:00007189", "sets": ["849"]}, "author_link": ["17409", "17410", "17411", "17412", "17413", "17414", "17415", "17416"], "item_1628147817048": {"attribute_name": "出版タイプ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_970fb48d4fbd8a85", "subitem_version_type": "VoR"}]}, "item_6_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2013-05", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "5", "bibliographicPageEnd": "607", "bibliographicPageStart": "599", "bibliographicVolumeNumber": "60", "bibliographic_titles": [{"bibliographic_title": "ENDOCRINE JOURNAL"}]}]}, "item_6_description_19": {"attribute_name": "内容記述", "attribute_value_mlt": [{"subitem_description": "信州大学博士（医学）・学位論文・平成24年3月31日授与（甲第936号）・武井 真大", "subitem_description_type": "Other"}]}, "item_6_description_20": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "In pancreatic beta-cells, glucose metabolism leads to closure of ATP sensitive K+ channels (K-ATP channel) and Ca2+ influx, which is regarded as a required step for triggering of insulin release. Here, we demonstrate that glucose triggers rapid insulin release independent from its action on K-ATP channels given the cellular cAMP is elevated. We measured insulin release from rat pancreatic islets by static and perifusion experiments. Changes in cytosolic free Ca2+ concentration ([Ca2+](i)) were monitored using fura-2 loaded rat pancreatic beta-cells. Glucose-induced insulin release was abolished when Ca2+ influx was inhibited by a combination of 250 mu M diazoxide, an opener of K-ATP channel, and 10 mu M nifedipine, a blocker of L-type voltage-dependent Ca2+ channels. However, with both nifedipine and diazoxide, glucose induced a 5-fold increase in insulin release in the presence of 10 mu M forskolin, an activator of adenylyl cyclase. In the presence of diazoxide, nifedipine, and forskolin, 22 mM glucose sharply increased the rate of insulin release within 2 min which peaked at 6 min: this was followed by a further gradual increase in insulin release. In contrast, it lowered [Ca2+](i) with a nadir at 2-3 min followed by a gradual increase in [Ca2+](i). The glucose effect was mimicked by 20 mM alpha-ketoisocaproic acid, a mitochondrial fuel, and it was nullified by 2 mM sodium azide, an inhibitor of mitochondrial electron transport. Cerulenin, an inhibitor of protein acylation, decreased the glucose effect. In conclusion, a rise in [Ca2+](i) through voltage-dependent Ca2+ channels is not mandatory for glucose-induced triggering of insulin release.", "subitem_description_type": "Abstract"}]}, "item_6_description_30": {"attribute_name": "資源タイプ（コンテンツの種類）", "attribute_value_mlt": [{"subitem_description": "Article", "subitem_description_type": "Other"}]}, "item_6_description_5": {"attribute_name": "引用", "attribute_value_mlt": [{"subitem_description": "ENDOCRINE JOURNAL. 60(5):599-607 (2013)", "subitem_description_type": "Other"}]}, "item_6_publisher_4": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "JAPAN ENDOCRINE SOC"}]}, "item_6_relation_48": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "10.1507/endocrj.EJ12-0388"}], "subitem_relation_type_id": {"subitem_relation_type_id_text": "https://doi.org/10.1507/endocrj.EJ12-0388", "subitem_relation_type_select": "DOI"}}]}, "item_6_select_64": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_select_item": "publisher"}]}, "item_6_source_id_39": {"attribute_name": "NII ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "0918-8959", "subitem_source_identifier_type": "ISSN"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Takei,  Masahiro"}], "nameIdentifiers": [{"nameIdentifier": "17409", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Dezaki,  Katsuya"}], "nameIdentifiers": [{"nameIdentifier": "17410", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Ishii,  Hiroaki"}], "nameIdentifiers": [{"nameIdentifier": "17411", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Nishio,  Shin-ichi"}], "nameIdentifiers": [{"nameIdentifier": "17412", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Sato,  Yoshihiko"}], "nameIdentifiers": [{"nameIdentifier": "17413", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Suzuki,  Satoru"}], "nameIdentifiers": [{"nameIdentifier": "17414", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Yada,  Toshihiko"}], "nameIdentifiers": [{"nameIdentifier": "17415", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Komatsu,  Mitsuhisa"}], "nameIdentifiers": [{"nameIdentifier": "17416", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2015-09-25"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "H24Kou936_Takei.pdf", "filesize": [{"value": "2.1 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_note", "mimetype": "application/pdf", "size": 2100000.0, "url": {"label": "H24Kou936_Takei.pdf", "url": "https://soar-ir.repo.nii.ac.jp/record/7189/files/H24Kou936_Takei.pdf"}, "version_id": "628a0e0c-76b1-4424-8fa6-f92ba5049c3b"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "A new experimental model of ATP-sensitive K+ channel-independent insulinotropic action of glucose: a permissive role of cAMP for triggering of insulin release from rat pancreatic beta-cells", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "A new experimental model of ATP-sensitive K+ channel-independent insulinotropic action of glucose: a permissive role of cAMP for triggering of insulin release from rat pancreatic beta-cells", "subitem_title_language": "en"}]}, "item_type_id": "6", "owner": "1", "path": ["849"], "permalink_uri": "http://hdl.handle.net/10091/17659", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2014-06-27"}, "publish_date": "2014-06-27", "publish_status": "0", "recid": "7189", "relation": {}, "relation_version_is_last": true, "title": ["A new experimental model of ATP-sensitive K+ channel-independent insulinotropic action of glucose: a permissive role of cAMP for triggering of insulin release from rat pancreatic beta-cells"], "weko_shared_id": -1}