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The purified peptide was supposed to be located at or near the glutamine-rich region 202 to 222 of the glycinin G4 subunit. The peptide significantly increased the number of CD8(+), CD11b(+), and CD49b(+) cells in C3H/HeN mouse spleen cell cultures, while 2 chemically synthesized glutamine-rich peptides corresponding to residues 202 to 213 (QQQQQQKSHGGR) and residues 214 to 225 (KQGQHQQEEEEE) of the glycinin G4 subunit increased the number of interleukin (IL)-12(+)CD11b(+) cells. The peptide 202-213 also significantly increased the number of CD49b(+), IL-2(+)CD4(+), and interferon-gamma(+)CD4(+) cells and stimulated the cytotoxic activity of spleen cells toward the human erythroleukemia cell line K562. These results indicate that the glutamine-rich region of the soybean glycinin G4 subunit stimulates the cellular immune system in mouse spleen cell cultures."}]}, "item_creator": {"attribute_name": "\u8457\u8005", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Egusa,  Shintaro"}], "nameIdentifiers": [{"nameIdentifier": "32753", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Otani,  Hajime"}], "nameIdentifiers": [{"nameIdentifier": "32754", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "\u30d5\u30a1\u30a4\u30eb\u60c5\u5831", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2015-09-25"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "characterization_cellular_immunostimulating_Peptide.pdf", "filesize": [{"value": "623.0 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 623000.0, "url": {"label": "characterization_cellular_immunostimulating_Peptide.pdf", "url": "https://soar-ir.repo.nii.ac.jp/record/10351/files/characterization_cellular_immunostimulating_Peptide.pdf"}, "version_id": "78079a6e-a199-4216-a3b8-d4b191edf7ba"}]}, "item_keyword": {"attribute_name": "\u30ad\u30fc\u30ef\u30fc\u30c9", "attribute_value_mlt": [{"subitem_subject": "glycinin G4", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Peptidase R", "subitem_subject_scheme": "Other"}, {"subitem_subject": "cellular immunostimulation", "subitem_subject_scheme": "Other"}, {"subitem_subject": "glutamine-rich peptide", "subitem_subject_scheme": "Other"}, {"subitem_subject": "cytotoxic activity", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "\u8a00\u8a9e", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Characterization of a Cellular Immunostimulating Peptide from a Soybean Protein Fraction Digested with Peptidase R", "item_titles": {"attribute_name": "\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_title": "Characterization of a Cellular Immunostimulating Peptide from a Soybean Protein Fraction Digested with Peptidase R"}]}, "item_type_id": "6", "owner": "1", "path": ["1016/1017"], "permalink_uri": "http://hdl.handle.net/10091/10765", "pubdate": {"attribute_name": "\u516c\u958b\u65e5", "attribute_name_i18n": "\u516c\u958b\u65e5", "attribute_value": "2010-11-15"}, "publish_date": "2010-11-15", "publish_status": "0", "recid": "10351", "relation": {}, "relation_version_is_last": true, "title": ["Characterization of a Cellular Immunostimulating Peptide from a Soybean Protein Fraction Digested with Peptidase R"], "weko_shared_id": null}