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Transformation of Tobacco Cultured Cell by Particle Bombardment : Expression of Phenylalanine Ammonia-lyase Gene in Transgenic Tobacco Cells.
http://hdl.handle.net/10091/4588
http://hdl.handle.net/10091/4588c7e6c230-4dc2-4fa1-a958-c4cdfbe3557f
名前 / ファイル | ライセンス | アクション |
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pb12_165.pdf (885.2 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2010-02-12 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Transformation of Tobacco Cultured Cell by Particle Bombardment : Expression of Phenylalanine Ammonia-lyase Gene in Transgenic Tobacco Cells. | |||||
言語 | ||||||
言語 | eng | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | https://doi.org/10.5511/plantbiotechnology1984.12.165 | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | journal article | |||||
その他(別言語等)のタイトル | ||||||
その他のタイトル | パーティクルガンによるタバコ培養細胞への遺伝子導入 : phenylalanine ammonia-lyase(PAL)cDNAの導入と遺伝子発現 | |||||
著者 |
Nagai, N
× Nagai, N× Yanagisawa, K× Mizuno, K× Imura, H× Taguchi, G× Shimosaka, M× Shibata, D× Okazaki, M |
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信州大学研究者総覧へのリンク | ||||||
氏名 | Taguchi, G | |||||
URL | http://soar-rd.shinshu-u.ac.jp/profile/ja.uCDpjekV.html | |||||
信州大学研究者総覧へのリンク | ||||||
氏名 | Shimosaka, M | |||||
URL | http://soar-rd.shinshu-u.ac.jp/profile/ja.ZekmjekV.html | |||||
出版者 | ||||||
出版者 | 日本植物細胞分子生物学会 | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Plant tissue culture letters. 12(2):165-171 (1995) | |||||
書誌情報 |
Plant tissue culture letters 巻 12, 号 2, p. 165-171, 発行日 1995-08 |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | The plasmid DNA containing a NPTⅡ as a selectable marker gene and a tobacco PAL cDNA combined with the CaMV 35S promoter and NOS terminator was transformed into tobacco cells by a particle bombardment device based on flowing helium. The most effcient transformation was achieved when the amount of DNA-coated tungsten particle was 0.2㎎ per projectile, the amount of plasmid DNA was 4μg/㎎ of tungsten particles, the helium pressure accelerated was 3㎏/c㎡, and the distance between the sample and the projectile was 15cm. Genetic analysis of kanamycin-resistant transformants obtained showed that the PAL cDNA construct integrated into the genome of tobacco cells. PAL activity of the transformant increased almost 4-fold and scopoletin content increased more than 2-fold as compared to nontrasformed cells. | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | ヘリウムガス圧式パーティクルガンにより,タバコ培養細胞のPALcDNAを同細胞へ再導入し,形質転換体を得た.タバコ培養細胞のPALcDNAをカリフラワーモザイクウイルス(CaMV)の35Sプロモーターとノパリン合成酵素(NOS)遺伝子のターミネーターに接続し,ネオマイシンホスホトランスフェラーゼⅡ(NPTⅡ)遺伝子とともにタバコ培養細胞へ導入し,形質転換体をカナマイシン培地で選抜した.また,パーティクルガンによる遺伝子導入の最適条件としては,加速圧力3㎏/c㎡,0.2㎎タングステン粒子/プロジェクタイル,4μg/㎎タングステン粒子,プロジェクタイルからサンプルまでの距離は15㎝であった.そして得られた形質転換体の中には,非形質転換体と比較して,PAL酵素活性で4倍,スコポレチン生成量で2倍以上のものが確認された. | |||||
資源タイプ(コンテンツの種類) | ||||||
内容記述タイプ | Other | |||||
内容記述 | Article | |||||
ISSN | ||||||
収録物識別子タイプ | PISSN | |||||
収録物識別子 | 0289-5773 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AN10050931 | |||||
出版タイプ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 |