Item type |
学術雑誌論文 / Journal Article(1) |
公開日 |
2022-01-27 |
タイトル |
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タイトル |
Methylxanthine Derivative-Rich Cacao Extract Suppresses Differentiation of Adipocytes through Downregulation of PPARγ and C/EBPs |
言語 |
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言語 |
eng |
DOI |
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関連タイプ |
isVersionOf |
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関連識別子 |
https://doi.org/10.3177/jnsv.64.151 |
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関連名称 |
10.3177/jnsv.64.151 |
キーワード |
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主題 |
cacao, methylxanthine derivatives, PPARγ, C/EBPs, adipocyte differentiation |
資源タイプ |
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資源 |
http://purl.org/coar/resource_type/c_6501 |
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タイプ |
journal article |
著者 |
Yamashita, Yoko
Mitani, Takakazu
Wang, Liuqing
Ashida, Hitoshi
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信州大学研究者総覧へのリンク |
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氏名 |
三谷, 塁一 |
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URL |
https://soar-rd.shinshu-u.ac.jp/profile/ja.ypcCPUkh.html |
出版者 |
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出版者 |
一般財団法人 学会誌刊行センター |
引用 |
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内容記述 |
Journal of Nutritional Science and Vitaminology. 64(2): 151-160. (2018) |
書誌情報 |
Journal of Nutritional Science and Vitaminology
巻 64,
号 2,
p. 151-160,
発行日 2018-04-30
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抄録 |
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内容記述 |
Abstract Cacao extract (CE) consumption has beneficial effects on human health, such as lowering the risk of obesity. However, the underlying molecular mechanism for the anti-obesity effect of CE remains incompletely understood. Here, we used a 50% aqueous alcohol extract of cacao mass, which is rich in methylxanthine derivatives (about 11%) and poor in flavan-3-ols (less than 1%), and assessed the suppression effects of this extract on adipocyte differentiation to investigate the anti-obesity mechanism. CE dose-dependently decreased fat accumulation in 3T3-L1 cells without affecting cell viability. CE also dose-dependently decreased the protein and gene expression levels of two adipogenesis-related transcription factors, peroxisome proliferator-activated receptor gamma (PPARγ) and CCAAT/enhancer-binding proteins (C/EBPs). Moreover, CE decreased protein expression levels of sterol regulatory element-binding protein 1 (SREBP1) and its downstream fatty acid synthase (FAS), which was accompanied by the retained localization of SREBP1 in the cytoplasm of 3T3-L1 cells. After ICR mice were fed a diet containing 1% CE for 1 wk, their white adipose tissue weight was lower, whereas their brown adipose tissue weight was higher compared with those of control animals. Additionally, the protein expression levels of PPARγ, C/EBPs, SREBP1, and FAS in the white adipose tissue of these mice were also lower than those in control animals. In contrast, diet supplementation with CE induced higher levels of phosphorylated AMP-activated protein kinase (AMPK) and its downstream acetyl-CoA carboxylase. In conclusion, methylxanthine derivative-rich CE decreases fat accumulation in adipocytes by downregulating the expression of the adipocyte differentiation master regulators through the activation of AMPK. |
資源タイプ(コンテンツの種類) |
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内容記述 |
Article |
ISSN |
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収録物識別子タイプ |
EISSN |
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収録物識別子 |
1881-7742 |
書誌レコードID |
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収録物識別子タイプ |
NCID |
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収録物識別子 |
AA12108939 |
出版タイプ |
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出版タイプ |
AM |
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出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |