{"_buckets": {"deposit": "60ba368a-c6ac-48e0-8c62-4613e93794e0"}, "_deposit": {"id": "2000704", "owners": [1], "pid": {"revision_id": 0, "type": "depid", "value": "2000704"}, "status": "published"}, "_oai": {"id": "oai:soar-ir.repo.nii.ac.jp:02000704", "sets": ["1017"]}, "author_link": [], "control_number": "2000704", "item_1628147817048": {"attribute_name": "出版タイプ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_ab4af688f83e57aa", "subitem_version_type": "AM"}]}, "item_6_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2018-04-30", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "2", "bibliographicPageEnd": "160", "bibliographicPageStart": "151", "bibliographicVolumeNumber": "64", "bibliographic_titles": [{"bibliographic_title": "Journal of Nutritional Science and Vitaminology"}]}]}, "item_6_description_20": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "Abstract\nCacao extract (CE) consumption has beneficial effects on human health, such as lowering the risk of obesity. However, the underlying molecular mechanism for the anti-obesity effect of CE remains incompletely understood. Here, we used a 50% aqueous alcohol extract of cacao mass, which is rich in methylxanthine derivatives (about 11%) and poor in flavan-3-ols (less than 1%), and assessed the suppression effects of this extract on adipocyte differentiation to investigate the anti-obesity mechanism. CE dose-dependently decreased fat accumulation in 3T3-L1 cells without affecting cell viability. CE also dose-dependently decreased the protein and gene expression levels of two adipogenesis-related transcription factors, peroxisome proliferator-activated receptor gamma (PPARγ) and CCAAT/enhancer-binding proteins (C/EBPs). Moreover, CE decreased protein expression levels of sterol regulatory element-binding protein 1 (SREBP1) and its downstream fatty acid synthase (FAS), which was accompanied by the retained localization of SREBP1 in the cytoplasm of 3T3-L1 cells. After ICR mice were fed a diet containing 1% CE for 1 wk, their white adipose tissue weight was lower, whereas their brown adipose tissue weight was higher compared with those of control animals. Additionally, the protein expression levels of PPARγ, C/EBPs, SREBP1, and FAS in the white adipose tissue of these mice were also lower than those in control animals. In contrast, diet supplementation with CE induced higher levels of phosphorylated AMP-activated protein kinase (AMPK) and its downstream acetyl-CoA carboxylase. In conclusion, methylxanthine derivative-rich CE decreases fat accumulation in adipocytes by downregulating the expression of the adipocyte differentiation master regulators through the activation of AMPK.", "subitem_description_type": "Abstract"}]}, "item_6_description_30": {"attribute_name": "資源タイプ（コンテンツの種類）", "attribute_value_mlt": [{"subitem_description": "Article", "subitem_description_type": "Other"}]}, "item_6_description_5": {"attribute_name": "引用", "attribute_value_mlt": [{"subitem_description": "Journal of Nutritional Science and Vitaminology. 64(2): 151-160. (2018)", "subitem_description_type": "Other"}]}, "item_6_link_3": {"attribute_name": "信州大学研究者総覧へのリンク", "attribute_value_mlt": [{"subitem_link_text": "三谷, 塁一", "subitem_link_url": "https://soar-rd.shinshu-u.ac.jp/profile/ja.ypcCPUkh.html"}]}, "item_6_publisher_4": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "一般財団法人 学会誌刊行センター"}]}, "item_6_relation_48": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "10.3177/jnsv.64.151"}], "subitem_relation_type": "isVersionOf", "subitem_relation_type_id": {"subitem_relation_type_id_text": "https://doi.org/10.3177/jnsv.64.151", "subitem_relation_type_select": "DOI"}}]}, "item_6_select_64": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_select_item": "author"}]}, "item_6_source_id_35": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "1881-7742", "subitem_source_identifier_type": "EISSN"}]}, "item_6_source_id_39": {"attribute_name": "NII ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "1881-7742", "subitem_source_identifier_type": "EISSN"}]}, "item_6_source_id_40": {"attribute_name": "書誌レコードID", "attribute_value_mlt": [{"subitem_source_identifier": "AA12108939", "subitem_source_identifier_type": "NCID"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Yamashita, Yoko", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Mitani, Takakazu", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Wang, Liuqing", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Ashida, Hitoshi", "creatorNameLang": "en"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2022-01-27"}], "download_preview_message": "", "file_order": 0, "filename": "JNSV64-2-151.pdf", "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 0, "url": {"label": "JNSV64-2-151.pdf", "url": "https://soar-ir.repo.nii.ac.jp/record/2000704/files/JNSV64-2-151.pdf"}, "version_id": "b4a7052d-b246-4939-b89b-192aa90a3a30"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "cacao", "subitem_subject_scheme": "Other"}, {"subitem_subject": "methylxanthine derivatives", "subitem_subject_scheme": "Other"}, {"subitem_subject": "PPARγ", "subitem_subject_scheme": "Other"}, {"subitem_subject": "C/EBPs", "subitem_subject_scheme": "Other"}, {"subitem_subject": "adipocyte differentiation", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Methylxanthine Derivative-Rich Cacao Extract Suppresses Differentiation of Adipocytes through Downregulation of PPARγ and C/EBPs", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Methylxanthine Derivative-Rich Cacao Extract Suppresses Differentiation of Adipocytes through Downregulation of PPARγ and C/EBPs", "subitem_title_language": "en"}]}, "item_type_id": "6", "owner": "1", "path": ["1017"], "permalink_uri": "http://hdl.handle.net/10091/0002000704", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2022-01-27"}, "publish_date": "2022-01-27", "publish_status": "0", "recid": "2000704", "relation": {}, "relation_version_is_last": true, "title": ["Methylxanthine Derivative-Rich Cacao Extract Suppresses Differentiation of Adipocytes through Downregulation of PPARγ and C/EBPs"], "weko_shared_id": -1}