Item type |
学術雑誌論文 / Journal Article(1) |
公開日 |
2022-11-14 |
タイトル |
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タイトル |
Genomic characterisation and epidemiology of nosocomial Serratia marcescens isolates resistant to ceftazidime and their plasmids mediating rare bla TEM-61 |
言語 |
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言語 |
eng |
DOI |
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関連タイプ |
isIdenticalTo |
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関連識別子 |
https://doi.org/10.1016/j.jgar.2021.03.010 |
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関連名称 |
10.1016/j.jgar.2021.03.010 |
キーワード |
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主題 |
Serratia marcescens, Nosocomial spread, Tn1, Ceftazidime resistance, bla(TEM-61) |
資源タイプ |
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資源 |
http://purl.org/coar/resource_type/c_6501 |
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タイプ |
journal article |
著者 |
Hayashi, Wataru
Yoshida, Satoshi
Izumi, Katsutoshi
Koide, Shota
Soga, Eiji
Takizawa, Shino
Arakawa, Yoshichika
Nagano, Yukiko
Nagano, Noriyuki
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信州大学研究者総覧へのリンク |
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氏名 |
長野, 由紀子 |
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URL |
https://soar-rd.shinshu-u.ac.jp/profile/ja.OUAeumyC.html |
信州大学研究者総覧へのリンク |
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氏名 |
長野, 則之 |
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URL |
https://soar-rd.shinshu-u.ac.jp/profile/ja.HULFumkh.html |
出版者 |
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出版者 |
Elsevier |
引用 |
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内容記述 |
Journal of Global Antimicrobial Resistance 25 : 124-131(2021) |
書誌情報 |
Journal of Global Antimicrobial Resistance
巻 25,
p. 124-131,
発行日 2021-06
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抄録 |
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内容記述 |
Objectives: We determined the whole DNA sequences of plasmids carrying a rare extended-spectrum beta-lactamase gene (bla(TEM-61)) to precisely understand the spread of resistance among nosocomial Serratia marcescens populations. <br>Methods: Twenty non-duplicate ceftazidime-resistant S. marcescens nosocomial isolates (ceftazidime MICs, 32 to >128 mg/L) collected over 1 year were pulsotyped and nucleotide sequences of the bla(TEM-61) gene and its promoter region were determined. Twelve representative isolates were analysed by whole-genome sequencing. <br>Results: The 20 isolates comprised two distinct pulsotypes: I (14 isolates) and II (6 isolates). They all contained the bla(TEM-61) gene. A polymorphism in the repeat number of a 15-nucleotide sequence (5'-ATGTCATGATAATAA-3') was found in the promoter region of bla(TEM-61); two, three and four repeat units were found in 6,12 and 2 isolates, respectively. Single nucleotide polymorphism (SNP)-based phylogenetic analysis of 12 isolates revealed that 7 isolates of pulsotype I (12-44 SNP differences) and 5 isolates of pulsotype II (15-55 SNP differences) formed two distinct clusters of genotypes 1 and 2, respectively. All 12 isolates harboured a plasmid carrying the Tn1-bla(TEM-61) element, although they were slightly different in size (78 883 bp, 78 898 bp and 78 913 bp) owing to differences in the number of 15-bp repetitive sequences. A 42 542-bp broad-host-range plasmid carrying the Tn1-bla(TEM-61) element was also found in one of the isolates. Conclusions: We characterised a plasmid-encoded novel Tn1-bla(TEM-61) element and transposon-dependent mechanisms underlying the propagation of antibiotic resistance, together with repeated new polymorphic 15-bp units in the promoter of bla(TEM-61). (C) 2021 The Author(s). Published by Elsevier Ltd on behalf of International Society for Antimicrobial Chemotherapy. |
資源タイプ(コンテンツの種類) |
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内容記述 |
Article |
ISSN |
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収録物識別子タイプ |
EISSN |
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収録物識別子 |
2213-7173 |
PubMed |
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関連識別子 |
https://pubmed.ncbi.nlm.nih.gov/33762209/ |
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関連名称 |
33762209 |
権利 |
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権利情報 |
© 2021 The Author(s). Published by Elsevier Ltd on behalf of International Society for Antimicrobial Chemotherapy. This is an open access article under the CC BY-NC-ND license ( http://creativecommons.org/licenses/by-nc-nd/4.0/ ) |
出版タイプ |
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出版タイプ |
VoR |
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出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |